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1

Antagonistic bacteria in relation to Pseudomonas syringae pv. syringae occurring in necroses and cankers of sour cherry trees

100%
Sobiczewski P.
Fruit Science Reports
|
1987
|
tom 14
|
nr 4
2

The effect of conservation tillage on microorganism communities in the soil under onion cultivation

86%
Pieta D., Kesik T.
Electronic Journal of Polish Agricultural Universities. Series: Horticulture
|
2007
|
tom 10
|
nr 1
3

Changes in population of selected bacteria in the rhizosphere of potato under different farming systems

86%
Breza-Boruta B., Paluszak Z.
Electronic Journal of Polish Agricultural Universities. Series Agronomy
|
2003
|
tom 06
|
nr 2
The research was carried out on farms of the Bory Tucholskie Landscape Park near Tuchola. The aim of the research was to define the quantitative content of bacteria of Pseudomonas (the fluorescent subgroup), Arthrobacter, Azotobacter and the coryneform group in the rhizosphere of potato in different stages of its development. The “Aster” potato was grown in two farming systems: ecological and conventional. Microbiological analysis indicated that the bacteria of the coryneform group were the most numerous, and the next was Arthrobacter spp. More of investigated bacteria were isolated from the rhizosphere of potatoes grown in ecological farming system than in the conventional one. From the results obtained during three year’s study it is evident that the number of potentially antagonistic bacteria increased with the development of vegetation and was the highest in root zone area of the plants in harvest-mature tubers.
4
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Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

72%
Arzanlou M., Mousavi S., Bakhshi M., Khakvar R., Bandehagh A.
Journal of Plant Protection Research
|
2016
|
tom 56
|
nr 1
In the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.
5
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Antagonistic bacteria and their post-culture liquids in the protection of pea (Pisum sativum L.) from diseases

72%
Pieta D., Pastucha A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2008
|
tom 07
|
nr 4
The purpose of the paper was to establish the protective effect of the microbiological material prepared from the cells of Bacillus sp. Bk 30 and Pseudomonas sp. Pk 28 and their post-culture liquids against soil-borne pathogenic fungi. The use of biological control improved the emergences, healthiness of pea plants and their yielding. Despite using the microbiological material for seed dressing, pea plants were infected by Altenaria alternata, Ascochyta pisi, Botrytis cinerea, Fusarium culmorum, Fusarium oxysporum f. sp. pisi, Fusarium solani, Phoma exigua, Pythium irregulare, Rhizoctonia solani and Sclerotinia sclerotiorum. Among the applied microbiological material, the best protective effect against plant pathogens was observed for bacterium Bacillus sp. Bk 30 and its post-culture liquid.
6
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The effect of chitosan on the formation of microorganism communities in the rhizosphere soil of soybean

72%
Pastucha A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2005
|
tom 04
|
nr 2
The experiment was conducted in the years 1999–2001. The object of the studies was the rhizosphere soil of soybean ‘Polan’ cv., and 0.1% chitosan. This compound was used once (seed dressing), twice (seed dressing and seedling spraying), three times (seed dressing, seedling spraying and plant spraying at anthesis). The experiment also considered a control combination, i.e. without chitosan. The purpose of the studies was to determine the effect of chitosan on the qualitative and quantitative composition of microorganisms in rhizosphere soil with antagonists distinguished. The microbiological analysis of samples of soybean rhizosphere soil taken from particular experimental combinations pointed to different numbers of bacteria and fungi. The greatest total number of bacteria colonies and the smallest number of fungi colonies were characteristic of the rhizosphere soil of soybean after using chitosan three times. This sample of soil contained the greatest number of bacteria and fungi with antagonistic effect towards plant pathogens.
7
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Mozliwosc ograniczania wzrostu grzybow patogenicznych dla leszczyny przez bakterie antagonistyczne

58%
Krol Z., Machowicz-Stefaniak Z., Zalewska E.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2003
|
tom 02
|
nr 2
51-57
Amangst 138 epiphytic bacterial isolates originating from hazel leaves 12 isolates limited the growth of B. cinerea, 33 limited the growth of C. corylicola, 31 inhibited the growth of G. coryli and 36 isolates showed antagonistic activity against Phomopsis sp. in-vitro. It was found that 9 isolates limited the growth of all the pathogenes tested and 7 isolates inhibited the growth of at least 3 out of them. The abilities of bacterial isolates tested to limit of the pathgenes growth were higher after 4 days of the biotic influence than after 8 days. Isolates of bacteria with the highest inhibitory activity against the above – mentioned pathogenes were identified as Pseudomonas fluorescens, Pseudomonas sp., Bacillus sp. and Enterobacter, Citobacter, Klebsiella or Erwinia cypripedii.
8

Bakterie antagonistyczne i ekstrakty roślinne w ograniczaniu parcha jabłoni (Venturia inaequalis)

58%
Masny S., Mikicinski A., Sobiczewski P.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 1
Antagonistic activity of 63 bacterial isolates, originating from soil and apple leaves, against Venturia inaequalis was studied in dual culture on PDA medium. Based on measurement of inhibition zones, 16 isolates appeared to be most active. Three of them /57M, 59M (Pseudomonas spp.) and 103M (Collimonas sp.), significantly protected apple trees against apple scab on Gala trees in the net-glasshouse. Of 6 ethanol plant extracts (sage leaf, rhizomes of Potentilla, oak and willow bark and the aboveground parts of St. John's-wort and tetterwort), the highest activity in inhibition of V. inaequalis conidia germination, even after 10- and 20-fold dilution in water, showed extract from rhizomes of Potentilla. Applied at two concentrations (5 and 10%) on Gala apple trees it totally protected leaves against apple scab.
9

Preparation of new biofungicides using antagonistic bacteria and mineral compounds for controlling cotton seedling damping-off disease

58%
Ardakani S. S., Heydari A., Khorasani N. A., Arjmandi R., Ehteshami M.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 1
49-55
The overuse of chemical pesticides in agriculture has caused serious environmental problems and thus the demand for safer pesticides is increasing. One alternative is microbial pesticides that suppress plant pathogens via their microbial activities. As microbial pesticides are eco-friendly products, in this study we prepared four biological fungicides using two isolates of Pseudomonas fluorescens that included a talc-based powder and bentonite-based powder as mineral carriers. Then we evaluated the efficacy of these products in controlling cotton seedlings, damping-off, a fungal disease caused by Rhizoctonia solani at four intervals of 15, 30, 45 and 60 days after sowing the cotton seeds under greenhouse conditions. The results of greenhouse experiment on application of biofungicides showed that the efficacy of Bentonite-B1 treatment to control R. solani was promising as it increased the number of healthy seedlings 3.42 to 3.57 - fold and was much more effective than the carboxin/thiram fungicide in all stages.
10
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Wplyw mikroorganizmow antagonistycznych na ograniczanie porazenia soi przez grzyby chorpbotworcze przezywajace w glebie

58%
Pieta D., Patkowska E., Pastucha A., Belkot M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2002
|
tom 01
|
nr 1
23-30
The efficiency of protective activities of bacteria and fungi as Glycine max seeds dressing against the soilborne pathogenic fungi was evaluated in presented studies. The microbiological material was prepared from the antagonistic microorganisms strains of Bacillus spp., Pseudomonas spp., Gliocladium spp. and Trichoderma spp. The experiment was established in the field with soybean monoculture with infectious material of fungi naturally accumulated in the soil. Protective activities of applied microorganisms were determined on the ground of the number of grown plants, their healthiness and seeds crop quantity and quality. Obtained results showed that Trichoderma viride 254, T. harzianum 220 and Bacillus sp. 131 were the most effective for soybean protection. Gliocladium roseum 246, Gliocladium catenulatum 49 and Oxafun T Dressing turned out the least effective in their protective activities.
11
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The use of Biosept 33 SL, Biochikol 020 PC and Polyversum to control soybean [Glycine max [L.] Merrill] diseases against pathogens. Part II. Microorganism communities in the rhizosphere soil of soybean

58%
Pieta D.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2006
|
tom 05
|
nr 2
The purpose of the studies was to determine the effect of such preparations as Biochikol 020 PC, Polyversum and Biosept 33 SL on the formation of microorganisms communities in the rhizosphere soil of soybean. Results of the studies pointed to a positive effect of biopreparations on the increase number of cfu total bacteria, Bacillus spp. and Pseudomonas spp. After the application of biopreparations balance between pathogens and saprobionts was maintained in the rhizosphere soil of soybean. Such relation did not exist after using chemical preparations. Biopreparations increased the number of antagonistic bacteria – Bacillus spp., Pseudomonas spp. and fungi – Gliocladium spp., Trichoderma spp.
12
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The occurrence of antagonistic microorganisms in the soil after the cultivation of spring rye and common vetch

58%
Pieta D., Kesik T.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2005
|
tom 04
|
nr 1
The object of the studies were saprophytic bacteria and fungi isolated from the soil after the cultivation of spring rye and common vetch. The studies were conducted in laboratory conditions. The antagonistic effect of the isolates of Bacillus spp., Pseudomonas spp., Trichoderma hamatum and Trichoderma koningii was determined towards such pathogenic fungi as Alternaria alternata., Botrytis cinerea, Fusarium culmorum, Fusarium oxysporum, Fusarium solani, Phoma exigua var exigua, Pythium irregulare, Rhizoctonia solani and Sclerotinia sclerotiorum. It turned out on the basis of the obtained results that the highest antagonistic effect was characteristic of the isolates of Trichoderma koningii, while the smallest of Trichoderma hamatum. Among the examined bacteria isolates Pseudomonas spp. appeared to be a poorer antagonist than Bacillus spp.
13

Antagonistic properties of bacteria found in sugar beet leaf silage against pathogens naturally occurring in the mouth of cattle

51%
Galbas M., Dullin P., Selwet M.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 02
The aim of the study was to present antibacterial properties of bacteria found in sugar beet silage against Shigella. The experiment involved bullocks, from which the pathogenic bacteria were isolated, and microorganisms obtained from silage (without additives). It was found that pathogenic bacteria are inhibited by bacteria present in the silage. Experimental subjects included 10 bullocks (crosses of Limousine with Black and White Lowland (BWL) of 700 kg mean body weight. Silage was prepared from sugar beet leaves contaminated with soil. Plant material was ensiled in 6 PCV containers (barrels) of 200 dm3 in volume closed with a cover allowing the release of gaseous products. The ensiling process lasted 120 days. Samples for chemical and microbiological analyses were collected from three barrel depths (15, 30 and 45 cm) and were subsequently pooled to make a representative sample of 0.9 kg weight. The basic composition of the silage was determined in accordance with AOAC. The strain antagonistic to Shigella was identified by the molecular method: after isolating bacterial DNA, a PCR reaction was performed. The PCR analysis and the DNA sequence analysis showed that the organism which naturally occurs in sugar beet leaf silage and exhibits antagonistic properties to Shigella bacteria was Bacillus subtilis. Shigella spp., a pathogenic microorganism that is of particular concern to humans, was found in the mouth of cattle.
14
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Skutecznosc plynow pohodowlanych bakterii antagonistycznych w ochronie soi przed grzybami odglebowymi

51%
Patkowska E., Pastucha A.
Acta Agrobotanica
|
2005
|
tom 58
|
nr 2
125-136
The objective of the paper was to determine the effectiveness of post-culture liquids of Bacillus sp. Bsch 19 and Pseudomonas sp. Psch 16 in the protection of soybean from soil-borne fungi. The use of post-culture liquids of those bacteria in seed dressing positively affected the number, healthiness and yielding of soybean plants. The plants were mainly infected by Fusarium spp., Phoma exigua var. exigita, Rhizoctonia solani and Sclerotinia sclerotiorum. Those fungi were isolated much more rarely from the plants in combinations with the use of post-culture liquids of antagonistic bacteria as compared with the plants from the control combination, i.e. without seed dressing. A reverse relation was found for the occurrence of saprophytic fungi from the genera of Gliocladium, Penicillium and Trichoderma.
15

Antagonistyczne dzialanie bakterii zawartych w preparatach probiotycznych w odniesieniu do S. enteritidis i enteropatogennych szczepow E. coli

51%
Bednarski M., Kuczkowski M.
Acta Scientiarum Polonorum. Medicina Veterinaria
|
2006
|
tom 05
|
nr 2
83-90
16

Występowanie potencjalnie antagonistycznych bakterii w stosunku do patogenicznych grzybów na powierzchni korzeni ziemniaka (Solanum tuberosum)

44%
Breza-Boruta B., Paluszak Z.
Acta Agrobotanica
|
2003
|
tom 56
|
nr 1-2
The research was carried out on farms in Kiełpin in Bory Tucholskie Landscape Park in vicinity of Tuchola over 1997-1999. The purpose was to determine of quantitative composition of Pseudomonas and Arthrobacter genera, coryneform group and total count of bacteria on roots of potato. Aster cultivar of potato was cultivated in two systems: conventional and ecological. The microbiological analysis showed that the coryneform group dominated over other investigated groups of bacteria. The greatest number of bacteria was isolated from rhizop1ane of potatoes cultivated in ecological system. The results obtained during three years of the experiment show that the number of potentially antagonistic bacteria increased along with plant vegetation. The maximum number of these bacteria inhabited roots of older plants.
17

Biologiczna ochrona jabloni i grusz przed zaraza ogniowa [Erwinia amylovora] - szanse i ograniczenia

44%
Sobiczewski P., Mikicinski A., Berczynski S.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 2
330-337
18

Nowe możliwości biologicznej i chemicznej ochrony roślin przed zarazą ogniową (Erwinia amylovora)

44%
Mikicinski A., Sobiczewski P., Berczynski S.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 3
The efficacy against fire blight of 3 antagonistic bacterial isolates (48M, 59M, 141M), strain C9-1 ̶ the active component of BlightBan (USA), the yeast based preparation Blossom Protect and copper preparations (Flow Brix 380 SC, Cuproflow 375 SC, Miedzian 50 WP) was evaluated on apple blossoms on Idared trees and Jonagold cut off branches. The highest efficacy was demonstrated after protective use of all bacterial isolates (82.7–92.0%) and Blossom Protect (83.5%). Miedzian 50 WP at the dose of 1.5 kg/ha appeared to be low effective. Two treatments with Blossom Protect applied during the blooming of apple trees cultivars Golden Delicious and Jonagold did not cause phytotoxicity on apples assessed after harvest. Because most fungicides used for protection of apple against fungal diseases are toxic to yeast in Blossom Protect, it seems that this preparation could be applicable against fire blight only in organic growing of apple.
19
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The influence of after-crop plant mulch and onion cultivation on microorganism composition in soil

44%
Pieta D., Kesik T.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2008
|
tom 07
|
nr 3
The aim of the research was to determine the quantitative and qualitative composition of the communities of fungi and bacteria formed in the soil under the influence of onion cultivation, with consideration to various options of conserving cultivation, using spring rye and common vetch as after-crop cover plants. Moreover, in the laboratory tests, the occurrence of microorganisms characterizing by an antagonistic influence on pathogenic fungi with a facultative parasiting was established. As a result of the laboratory microbiological analysis it was found out that particular soil samples taken from under the cultivation of onion differed with the qualitative and quantitative composition of microorganisms. Spring rye stimulated the growth and development of microorganisms, especially antagonistic ones (Bacillus spp., Pseudomonas spp., Gliocladium spp., Trichoderma spp.), and common vetch caused an increase of the number of cfu of pathogens (Alternaria alternata, Fusarium culmorum, F. oxysporum, F. solani, Penicillium spp., Pythium irregulare).
20

Skuteczność mieszaniny bakterii Bacillus i Pseudomonas spp. w ochronie cyprysika Lawsona przed Phytophthora cinnamomi

37%
Ksiezniak A., Wroblewska B.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 529
Przeprowadzono badania nad możliwością ograniczania fytoftorozy cyprysika Lawsona przy zastosowaniu bakteryjnych izolatów uzyskanych z ryzosfery i wykazujących w testach laboratoryjnych antagonizm wobec Phytophthora cinnamomi. Wykazano, że mieszanina drobnoustrojów stosowana przed sadzeniem cyprysika Lawsona do inokulowanego podłoża, istotnie ograniczała rozwój fytoftorozy w ciągu 10-tygodniowej uprawy. Zastosowanie bakterii do opryskania roślin spowodowało słabsze ograniczenie rozwoju fytoftorozy po 10 tygodniach uprawy.
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