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The study aimed at assessing the sodium and potassium content in chosen tissues and organs of free-ranging European bisons in Białowieża Primeval Forest depending on the gender and age of animals. In order to determine the content of elements in parenchymal tissues, ribs and hair, the ICP-OES method was used. In the hooves, sodium and potassium were determined with the help of ICP-MS. The sodium content in organs and skin appendages varied from 0.30 in hair to 4.77 mg g-1 in ribs. In the analysis of the age effect, some significant differences were observed between the investigated groups in the sodium content of the hoof wall, namely, a higher mean value was noted in young individuals. The potassium content in the examined samples was within the range of 0.96 in the hoof wall to 3.63 mg g-1 in kidneys. Significant age dependent differences were noted only in the ribs. Sodium and potassium concentrations in the hoof wall were correlated in a highly significant way. Similar dependences also appeared between the content of sodium and potassium in kidneys and liver and kidneys and muscles. On the basis of the results it can be concluded that the status of sodium and potassium supply in the European bison from Białowieża Forest is adequate.
Thallium is a metal with a high rate of accumulation in the environment and at the same time there is a very large dispersion of it. Heavy metal studies and its bioaccumulation and concentration in organisms and health implications are still not well known. The aim of this study was to demonstrate the importance of this element as an intoxicant of living organisms, especially on laying hens. Four groups of ISA Brown hens were used in this study. Experimental groups received feed containing thallium as thallium sulfate (Tl₂SO₄). Concentrations and metal accumulation in the animals were determined periodically, analyzing the eggs and animal blood. After the experiment, concentrations of thallium in the tissues was determined post-mortem. The studies showed a proportional accumulation of thallium in the body of the animals in relation to exposure time, and doses with the highest concentrations in the bones, kidneys, muscles, liver, and blood.
Histological studies of the brain, lungs, liver, kidneys, heart, and the spleen were carried out in mice previously infected with 6 pathogenic strains of free-living amoebae of the genus Acanthamoeba. The potential virulence of the strains studied was determined on the basis of re-isolation of the amoebae from the organs of the inoculated animals and by the extent of the histopathological changes inflicted. The most virulent was strain AD16, affecting all organs of the inoculated mice, while the least virulent was strain AD148 re-isolated from the brain of a single mouse. The extent of the changes in the brain depended upon the amoebae strain, while in the remaining organs it also depended upon the duration of the infection.
The aim of the present study was to evaluate the ability of Ornithobacterium rhinotracheale (ORT) to colonize chosen organs of chicks infected intratracheally (group A1), or intravenously (group A2), with the use of bacteriological methods and PCR. The bacteriological methods enabled to reisolate ORT bacteria from trachea and lungs of the birds from group A1 only on day 3 and 6 after infection. The PCR technique additionally detected the bacterial genetic material in these organs on the 9th day after infection, and gave positive results in the samples from air sacs until the 6th day of the experiment. In birds infected intravenously (A2) ORT was reisolated from liver on day 3 and from spleen on day 3 and 6 after infection, whereas the reisolation from the tibiotarsal joint occurred during the entire experimental period. PCR enabled to detect the bacterial DNA in the liver, spleen and lungs of chickens until the 9th day after infection and in case of tibiotarsal joint during the whole time of the study.
Deoxynivalenol is one of mycotoxins that are most frequently determined in animal feed manufactured in Poland. The examination of histopathological lesions concomitant with deoxynivalenol intoxication is difficult because of the common, often synergistic, reaction of this mycotoxin with other toxins, such as zearalenone or ochratoxin A, which has a strong nephrotoxic activity. The possibility of estimating histopathological lesions in the course of intoxication with pure toxin at various doses is therefore of interest. Dosages used in this experiment relate to clinical cases observed in feeding the animals with whole ration feed obtained by processing feedingstuffs contaminated with Fusarium moulds. However, concerning the fact of one-shot administration of clinically pure toxin, the main question was if it was a sufficient dose to cause changes in the histopathological picture of gastrointestinal tract organs. The experiment was carried out on 12 nursery pigs of mixed breed (Polish White Large x Polish White Ear-pendent) with an average body weigh of 35 kg. The experimental nursery pigs were divided into 3 groups: group I (n=4) – control; group II (n=4) – DON administered at a dose of 0.2 mg/kg b.w.; group III (n=4) – DON administered at a dose of 0.4 mg/kg b.w. After slaughter of the animals, macroscopic examination was performed and segments of duodenum, jejunum, ileum, liver and mesenteric lymph nodes were sampled and assigned for histopathological examination. The results obtained equate to the clinically observed signs in swine production involving some nutrient metabolism disturbances in the gastrointestinal tract in the course of deoxynivalenol mycotoxicosis. Histopathological examination of segments of the duodenum, the jejunum, the ileum, the liver and the lymph nodes indicate that the regressive lesions are more expressed in the experimental group treated with the highest concentration of deoxynivalenol.
The presented investigations aimed at determining manganese status in the European bison free ranging in Białowieża primeval forest on the basis of the analysis of some chosen tissues and organs, considering the sex and age of the animals. The manganese status in the liver, kidneys, gastrocnemius muscle, hair, and hoof was determined. The material for analyses was obtained in the winter 2002 from animals eliminated within annual selection. The material originated from 20 European bisons aged from 5 months to 5 years. Animals were grouped according to sex and age (calves up to one year of age, animals aged over two years). The manganese content (except hoof) was determined using the ICP- OES method in the accredited laboratory. In the hoof samples the content of manganese was determined using the method ICP - MS in the same laboratory. The obtained results were analysed statistically with the Statistica programme. No statistically significant differences in the manganese content were observed depending on the sex and age of animals. The mean manganese content in the tissues and organs (n=20; mg·kg⁻¹ of fresh tissue) was as follows: liver - 10 ±1.2; kidneys - 4.5 ±1.2; ribs - below the sensitivity of the apparatus (<1 ppm of Mn in the solution); muscle - 2.3 ±0.4; hair - 15.2 ±4.3; hoof-3.0 ±2.2 (in dry matter).
Bioaccumulation of nickel and the effect of nickel on the iron level in the tissues and organs of wels catfish, Silurus glanis L. were studied in the course of the present work. A number of blood parameters were determined e.g. erythrocyte count (RBC), leukocyte count (WBC), haemoglobin concentration (Hb), haematocrit value (Ht), and erythrocyte indices (MCH, MCV, MCHC) were calculated. On stained blood smears erythrocytes and leukocytes were differentiated. Effect of nickel on the activity of alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the blood serum was studied.
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