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The objective of the study was to determine if there were differences in Cr, Mn, Cu, Cd, Pb and Ni levels in coat hair and hoof horn between Polish Konik horses maintained in stabled and reserve systems in Roztocze National Park in Poland. Summer and winter feeding seasons (seasons of the year in case of hooves) and age of horses were taken into account. 107 samples of hair and hooves, as well as all kinds of feed and sources of water available for the horses were examined. The results of the study indicate that the concentration of the elements in the coat hair, hoof horn, feeds and water is usually too low to show possible influence of the system of maintenance in feeding seasons and age effect. The reserve horses are not more exposed to heavy metal contamination than the stabled horses. In the hair, solely Mn level is affected by the systems of maintenance in summer and winter feeding seasons: during winter it is higher, particularly in the stabled system, than during summer. In hooves, Cr concentration is higher in stabled horses than in reserve horses and conversely, Cu content is higher in the reserve horses. Mn content is age-affected both in hair and hooves, likewise is Cu content in the hooves. The results indicate a necessity of Cu compound supplementation in feeding the horses in both systems of maintenance.
The aim of the research was to develop a method for determining the species affinity of animal hair by determining its melting temperature. The research material was selected from hair samples of livestock and wild animals. We investigated 170 hairs of 16 animal species and humans. The hair melting point was determined by an automated system for melting temperature determination Opti Melt (MPA100). In the present study, we suggest that species affiliation can be effectively determined by examining physical properties of hair, in particular by determining its melting temperature. The hair melting temperatures for different animal species are in different ranges, but the initial melting points for hair of certain animal species are similar. For example, the initial melting point for cat, sheep, hare, rabbit, and rat hair ranged from 100 to 111°C, and the hair of the wolf, bear, and fox began to melt at 139.2 to 141.2°C. The results of this research make it possible to determine the species affinity of animals according to their hair melting temperature, which had not been done before. The proposed method yields rapid results and can be used as an auxiliary method in ambiguous cases in which data obtained by microscopic examination are insufficient. It will greatly facilitate the work of forensic specialists, enriching the arsenal of available methods
Human or animal hair is considered to be a good indicator of the content of bioelements or toxic elements in human and animal organism. Its analysis is a good alternative for the arduous obtaining blood or biopsy samples. The aim of the performed research was the assessment of some chosen bioelements in the organism of European bison on the basis of their analysis in hair. The investigation material comprised hair samples obtained from 22 animals. All animals were divided into groups according to gender (males, females) and age (calves up to one year of age and animals older than 2 years). Samples were mineralized in nitric acid, under pressure in the microwave apparatus. The content of phosphorus, sulphur, magnesium, iron, titanium and vanadium were determined in hair samples. The content of these elements was determined using the ICP-AES method in accredited laboratory. The accuracy of determinations was tested using the standard reference material. The mean content of phosphorus in hair amounted to 245.14 mg · kg–1, SD 65.00, magnesium 97.32 mg · kg–1, SD 33.16, iron 119.48 mg · kg–1, SD 83.31 and titanium 2.368 mg · kg–1, SD 2.097. In case of these elements, differences depending on gender and age were statistically insignificant. Mean content of sulphur in the European bison hair amounted to 3.41% with equalized content in the herd (SD 0.22%). Here also statistically insignificant differences depending on gender were observed. However, a statistically significant difference (p ≤ 0,05) was demonstrated which depended on animal age. Mature European bisons had more sulphur in hair as compared to calves. The concentration of vanadium (0.362 mg · kg–1, SD 0.396 on the average) significantly differed in hair depending on the European bison age with much higher values in adult animals (calves 0.260 mg · kg–1, animals older than 2 years 0.686 mg · kg–1). Similar data were obtained while investigating hair of sea mammals.
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