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Polychlorinated biphenyls (PCBs) due to their lipophilic properties can be easily accumulated in animal and human body and elicit diverse effects causing impairment of reproductive processes. Since these compounds were not be able to affect directly the luteal steroidogenesis, the aim of the present study was to verify hypothesis that PCBs can impair the effect of LH on the secretory function of luteal cells. Bovine luteal cells from different stages of the oestrous cycle (days 1-5, 6-10, 11-15 and 16-18) were exposed for 72h to various congeners of PCBs (PCB 126, PCB 77 and PCB 153) at the doses of 1, 10 or 100 ng/ml, in the presence or absence of LH (100 ng/ml), to determine the possible effect of these compounds on progesterone (P4) and ovarian oxytocin (OT) secretion. Only PCB 77 on days 1-5 and 16-18 increased P4 secretion. All PCBs decreased LH-simulated secretion of P4 from luteal cells obtained from all days of luteal phase. Dioxin-like congener (PCB 126) inhibited (P<0.05) the most evidently LH effect on P4 secretion. All congeners, except the lower doses of PCB 126, increased (P<0.05) OT secretion. They can also increase LH-stimulated secretion of OT, but the effect was dependent on the congener used and on the phase of oestrous cycle. On days 1-5 and 10-15, PCB 126 diminished LH-stimulated effect on OT secretion from luteal cells. PCB 77 (mimickig both dioxin and estradiol effect) in the higher doses, amplified effect of LH-stimulated OT secretion, while on all other days it diminished LH influence. PCB 153, which has estrogen-like properties, amplified LH effect on OT secretion during all studied days of the cycle. We conclude that PCBs (supposedly via estrogen and arylhydrocarbon - AhR receptor) may directly affect LH-stimulated function of CL. This does not appear to be a direct adverse effect on luteal steroidogenesis, but rather indirect on OT secretion from or within CL.
The aim of the study was to investigate into the possibility of evaluating arctic fox fur basing on the correlation of laboratory measurements of hairs collected from various parts of the coat. The material involved samples of prime fur hairs of 20 two-year-old females of the blue arctic fox, collected at the end of January. The samples were cut by the skin at six places of the body, namely: the head (between the ears), back (in the middle between the tail base and the neck base), the side (below the place of sampling located on the back), belly (mid between the base of the front limbs and the vulva), the tail (in the middle of the dorsal side), as well as the shank. The samples were subjected to macro- and microscopic measurements. The results indicate that fur coat of arctic foxes is more dense, longer, and usually more intensely colored on the back, as compared to the belly. The hairs on the head and the limbs are much shorter and contain more awn hairs. The results reveal a very weak correlation of the morphological fur characteristics between different parts of the body. It has been found that it is impossible to objectively evaluate the basic structural characteristics and the dark tops of hairs in the arctic fox on the basis of a sample of hair from a single part of the body.
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