The aim of these studies was the clonal analysis of 48 S. aureus strains, isolated from milk samples from 48 cows with clinical and subclinical mastitis. Specific markers were detected using PCR: gap gene as the genus marker for Staphylococcus spp. and nuc gene as the species marker for S. aureus. Clonal typing of S. aureus isolates was carried out using RAPD-PCR with two primers simultaneously. Clonal relatedness was determined on the basis of the number and molecular mass of RAPD amplicons and analyzed using the unweighted pair group method with arithmetic mean (UPGMA) and Jaccard’s similarity coefficient. On the basis of RAPD-PCR and clonal analysis employing the UPGMA method with Jaccard coefficient it was determined that all of the analyzed S. aureus strains were identical on the genome level. This indicates that only one bacterial clonal type was responsible for mastitis caused by S. aureus in the analyzed herd. This denotes that the virulence of S. aureus isolated from mastitis cases is very high, and that the elimination of this strain is very difficult. The research showed the usefulness of RAPD-PCR in interspecies S. aureus strains typization and the prospect of employing it in routine epidemiological diagnostics of mastitis in cows. The contribution of S. aureus strains representing one clonal type in the etiology of mastitis in cows from one herd was also confirmed.
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