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1

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5

100%
Dabrowski S., Pietrewicz-Kubicz E., Zablotna E., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
2

Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol

100%
Dabrowski S., Zablotna E., Pietrewicz-Kubicz D., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
3

Wypróbowanie warstwy tzw. “mikrosfer mokrych” w zbiorniku fermentacji beztlenowej

100%
Marciniak K.
Gazeta Cukrownicza
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1989
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tom 97
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nr 01
4
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Survival of BPV and Aujeszky's disease viruses in meat wastes subjected to different sanitization processes

80%
Paluszak Z., Lipowski A., Ligocka A.
Polish Journal of Veterinary Sciences
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2010
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tom 13
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nr 4
The effect of composting and anaerobic fermentations under meso- and thermophylic conditions (37° and 55℃) on the survival of bovine parvovirus (BPV) and Aujeszky’s disease viruse (ADV) in meat wastes has been examined in this study. Viruses were adsorbed on filters and introduced into carriers which were made of meat fragments of different sizes and bones or in the form of suspension they were introduced into the biomass in the course of processes of waste treatment. Carriers were removed at appropriate time intervals and virus titres were determined. The thermoresistant parvovirus survived for the longest time during mesophylic fermentation (almost 70 days), slightly shorter during composting (7-9.5 days depending on the type of carrier) and for the shortest time – at 55℃ (46-76 hours). Its inactivation rate was the fastest in a suspension, slower in meat and bone carriers. ADV inactivation proceeded considerably faster, as compared with BPV. Its active particles were not detected as early as in the 30th minute of thermophylic fermentation, the 6th hour of mesophylic fermentation and at the first sampling time during composting (at the 72nd hour). Total survival time ranged from 50 min to 13 hours. All the tested technologies enabled the effective elimination of ADV and on average twofold decrease in BPV titre. From the study conducted it follows that of both viruses, the BPV should be applied for validation processes of methods used in meat waste processing, particularly if this refers to methods where higher temperature is the factor inactivating pathogens.
5

Beztlenowa fermentacja ubocznych i odpadowych produktów przemysłu cukierniczego

80%
Buchholz K., Stoppok E., Arntz H. J.
Gazeta Cukrownicza
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1988
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tom 96
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nr 01
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