Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 44

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  amylase
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
α-Amylase has a wide range of applications in starch industries, i.e. baking, brewing, distillery, etc. The α-amylase production from Streptomyces erumpens MTCC 7317 immobilized cells was compared with that of free cells. The immobilized cells of S. erumpens in calcium alginate beads were more effective for production of α-amylase (12.2% more yield) than free cells. Response surface methodology (RSM) was used to evaluate the effect of main variables, i.e. incubation period, pH and temperature on enzyme production with immobilized cells. A full factorial Central Composite Design (CCD) was applied to study these main factors that affected α-amylase production. The experimental results showed that the optimum incubation period, pH and temperature were 36 h, 6.0 and 50°C, respectively for immobilized cells. Repeated batch fermentation of immobilized cells in shake flasks carried out in starch-beef extract medium showed that S. erumpens cells were physiologically active on the support even after four cycles of fermentation.
Production of enzymatic starch hydrolyzates is made by two-steps action of amylolytic enzymes on gelatinized starch granules – at the beginning by liquefying enzymes and next by saccharifying enzymes. Differences of optimal parameters of the enzyme action require the change of reaction conditions: temperature and pH.
Background. Acute pancreatitis morbidity has been rising annually all around the world. In case of acute necrotic pancreatitis, the mortality reaches 40% in the majority of hospitals. The therapy is effective if the efferent methods involve complex therapy of this disease. One such method is hemoperfusion, i.e. extracorporeal blood purification, which is widely used in toxicology. The authors of the article used hemoperfusion in a complex therapy in patients with severe acute pancreatitis. The following research presents an evaluation of the results of hemoperfusion used in a complex therapy of patients with acute pancreatitis. Material and methods. The study involved 38 patients with acute non-biliary moderately severe pancreatitis who were treated at an intensive care unit of Ternopil University Hospital in Ukraine. 18 patients were treated following the protocol for treatment of acute pancreatitis. In 20 patients, this treatment was additionally combined with hemoperfusion. We determined the levels of amylase, diastase, procalcitonin, bilirubin, malonic dialdehyde, and diene conjugates in blood serum and the level of leukocytes in the blood. Results. The levels of procalcitonin, amylase, bilirubin, leukocytes number, malonic dialdehyde, and diene conjugates were stabilised in patients of both groups, but in those who received hemoperfusion demonstrated much better results. These were manifested by significantly better levels of the amylase, bilirubin, creatinine, urea, procalcitonin, malonic dialdehyde, and diene conjugates in patients who received hemoperfusion (p< 0.005) than in the other group undergoing standard drug therapy. Conclusions. Hemoperfusion can be used as an effective method in the complex treatment of patients with acute pancreatitis.
Mineral and organic fertilization is one of the rtiost important factors affecting activity of soil enzymes. It is commonly accepted that organic fertilization is more beneficial to the soil biological activity than the mineral. The objectives of this investigation were to assay Corg, Ntot, Ptot content and to determine urease and amylase activities in the soil fertilised for a long time with various rates of farmyard manure and slurry. Soil samples were taken from the experiment established on a typical lessivé soil. FYM was used at the following rates: 20, 40, 60, 80 t ha-1 fresh weight once in the rotation on the plots with potatoes, while slurry was applied in four doses corresponding to manure fresh weight. Soil samples were taken after potato harvest in the 8th year after the experiment start-up from two depths: 5-15 and 25-35 cm of the soil profile. Usually, higher amounts of Corg and Ptot were found in the soil manured with FYM in comparison with the pig slurry-manured soil. These amounts increased when the doses of both fertilizers were the highest. The content of Corg ranged from 5.5 to 8.2 g kg-1 of soil when 80 t ha-1 of FYM was used. Total nitrogen ranged from 0.45 to 0.95 g kg-1 of soil, average for doses of both fertilizers, whereas total phosphorus content ranged from 0.26 to 0.48 g kg-1 of soil. The highest amylase activity was observed in the soil when FYM was used (0.48 ng starch g-1 l6 h-1) as compared to the soil where slurry was applied (0.41 g starch ug-1 16 h-1). No increase of amylase activity was observed when the doses of both fertilizers were the highest. A higher urease activity was noted when FYM was used than in the case of the soil manured with slurry.A decrease of urease activity was noted in the control samples (3.0 mg NH3 10 g-1 24 h-1), while the urease activity increased when the doses of both fertilizers were the highest. The highest activities were noted when 80 t ha-1 of FYM or slurry were used.
In order to compare the effect of organic and mineral fertilization of various soil types, long-term fertilization experiments frequently use indices, being a function of various parameters considered simultaneously; the content of organic carbon, microbiological parameters and soil enzymatic activity. The aim of the present research was to define soil fertility based on the values of Biochemical Soil Fertility Index (B). The index has been formulated based on the research results obtained: enzymatic activity, content of organic carbon and total nitrogen. The research material was sampled from topsoil of a many-year experiment which included varied organic and mineral fertilization, established on typical lessive soil. Soil samples were collected in 1998 from winter wheat stand, four times over the vegetation period. The following enzymes were determined: dehydrogenases, alkaline phosphatases, proteases and amylases. Additionally Corg and Ntotal and pH in 1 M KC.
Kinetics of amylolytic hydrolysis of autoclaved and extruded potato starch using alpha-amylase and pullulanase mixtures were described. Maltodextrins with dextrose equivalent of 3, 5, 8 and 12 were obtained and their sugar compositions with HPLC method were analyzed. The low molecular sugar (PD 1-2) and oligosaccharide (PD 3-8) contents as well as relationship between dextrose equivalent of maltodextrins and oligosaccharide contents were determined.
The studies on optimisation of citric acid biosynthesis by solid-state fermentation of sugar-beet pulp as the major material have shown that not only mono- and disaccharides initially present in the material but also the products of cellulose and hemicellulose hydrolyses were the carbon sources. Therefore, in parallel with optimisation of citric acid production, the studies were undertaken on the activity of ccllulases and dynamics of their synthesis by Aspergillus niger, the strain used in the process. The fermentations were carried out by non-mixed system using 1 L beakers and by mixed system using 4.5 L rotating drum bioreactor. The activity of CMC-ase and xylanases for the non-mixed system increased to about 22.0 IU/g and that of FP-ase stayed on the level of about 4 IU/g dry matter, whereas for the mixed system the CMC-ase activity increased to about 35 IU/g dry matter on day five and that of FP-ase stayed on a similar level of about 5 IU/g dry matter. The content of reducing compounds increased by almost four times (to 390 g/kg dry matter) on the second day of fermentation and decreased to about 140 g/kg dry matter on the fifth day of fermentation. Glucose was utilised most rapidly among monosaccharides, being followed by arabinose and in part by mannose.
Ghrelin, an endogenous ligand of the growth hormone secretagogue receptor, has been primarily isolated from the human and rat stomach. Ghrelin has been shown to stimulate appetite and fat deposition in adult rats and humans. The aim of this study was to investigate the effect of ghrelin administration on pancreatic growth in suckling, weaned and peripubertal seven week old rats. Rats were treated with saline or ghrelin (4, 8 or 16 nmol/kg/dose) intraperitoneally twice a day: suckling rats were treated for 7 or 14 days starting from the first postnatal day, three week old weaned rats and seven weeks old rats were treated for 5 days. Treatment with ghrelin did not affect animal weight in suckling or weaned rats, whereas in young seven week old rats, ghrelin caused a significant increase in body weight. Ghrelin decreased food intake in weaned rats; whereas in seven week old rats, food intake was enhanced. In suckling rats, ghrelin decreased the pancreatic weight, pancreatic amylase content, DNA synthesis and DNA content. In contrast, ghrelin increased pancreatic weight, DNA synthesis, DNA content and amylase content in weaned or young seven week old rats. Pancreatic blood flow was not affected by ghrelin in any group of rats tested. Ghrelin increased serum level of growth hormone in all rats. This effect was weak in suckling rats, higher in weaned and the highest in seven week old animals. Ghrelin did not affect serum level of insulin-like growth factor-1 (IGF-1) in suckling rats. In weaned and in seven week old rats, treatment with ghrelin caused increase in serum level of IGF-1. We conclude that ghrelin reduces pancreatic growth in suckling rats; whereas in weaned and young seven week old animals, treatment with ghrelin increases pancreatic growth. This biphasic effect of ghrelin in young animals on pancreatic growth seems to be related to age-dependent changes of the release of anabolic IGF-1.
20
Content available remote

Melatonin-induced protein synthesis in the rat parotid gland

58%
Melatonin occurs in large amounts in the intestinal mucosa and is released during a meal. Recent studies of ours reveal that exogenous melatonin evokes the in vivo secretion of protein and amylase from the rat parotid gland. The aim of the present study was to investigate the effect of melatonin on the protein synthesis of the parotid gland of pentobarbitone-anaesthetised rats as estimated by the rate of incorporation of [3H]leucine into trichloroacetic acid-insoluble material of the gland. Compared with the parotid protein synthesis (set at 100%) of those rats exposed to an intravenous infusion of melatonin (25 mg/kg during 1 hour), under muscarinic and - and ß-adrenoceptor blockade, the synthesis in the corresponding glands of saline-treated control rats was less (by 25%). The synthesis was also less when the melatonin administration was combined with the melatonin 2-preferring receptor antagonist luzindole (24%), the non-selective nitric oxide synthase inhibitor L-NAME (18%) and the neuronal nitric oxide synthase inhibitor N-PLA (21%). Almost all the melatonin receptor-mediated effect was due to nitric oxide generation via the activity of neuronal type nitric oxide synthase. The present findings lend further weight to the idea that salivary glandular activity associated with food intake is hormonally influenced and they also suggest clinical implications for melatonin in the treatment of xerostomia. Since melatonin is known to exert anti-inflammatory actions in the oral cavity, the stimulatory effect of melatonin may include the synthesis of proteins of importance for the oral defence.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.