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1

Bioactive preparations from alkaloid-rich lupin. Relation between chemical composition and biological activity

100%
Wysocki W., Gulewicz P., Aniszewski T., Ciesiolka D., Gulewicz K.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2001
|
tom 49
|
nr 2
2

Assessment of the effect of alpha-galactosides injected during embryogenesis on selected chicken traits

100%
Pilarski R., Bednarczyk M., Lisowski M., Rutkowski A., Bernacki Z., Wardenska M., Gulewicz K.
Folia Biologica
|
2005
|
tom 53
|
nr 1-2
3

Prebiotic properties of pea alpha-galactoside preparation applied to chicken per os

100%
Lisowski M., Lewandowska M., Bednarczyk M., Gulewicz P., Gulewicz K.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2003
|
tom 51
|
nr 4
4
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Changes in the alkaloid, alpha-galactoside and protein fractions content during germination of different lupin species

84%
Chilomer K., Zaleska K., Ciesiolka D., Gulewicz P., Frankiewicz A., Gulewicz K.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr 1
11-20
The objective of our studies were seeds of two lupin species Lupinus luteus L. and Lupinus angustifolius L. cvs. Lord and Graf respectively. Lupin seeds were germinated at 15 and 24°C and during two, three and four days. In the lupin sprouts antinutritional factors: alkaloids and raffinose family oligosaccharides (RFOs) and five nitrogen fractions: non protein (Nnp), albumin (A), globulin (G), glutelin and prolamin (Gt+P) and nitrogen residue fraction (Nr) were determined. The level of these compounds was compared with the proper ones of initial material (not germinated seeds). These studies showed that the germination process clearly affects the decrease of antinutritional factors: RFOs and alkaloids. The decrease level of these compounds depended on such factors like, lupin species and used germination conditions. It was found on the base of nitrogen analysis of particular protein fractions that the germination process of lupin seeds causes deep quantitative and qualitative changes in fractional composition of lupin proteins. It especially concerns the decrease of globulin and residual fraction content and distinct increase of Nnp fraction. The changes in other fractions were not so unequivocal in comparison with the mentioned above and depended on lupin species, temperature and time of germination. Qualitative changes of A, G and Gt+P fractions caused by germination were confirmed by gel electrophoresis (SDS-PAGE). The amino acid analysis of seeds and sprouts of Nnp fractions showed an increased content of Asp, Ser, Ala, Pronon essential amino acids (NEAA), and Val, Met, iLeu, Leu, Thressential amino acids (EAA). Simultaneously a decrease of Glu, Arg (NEAA), Phe, Lis, Cys (EAA) contents was observed. Generally the germination process causes the decrease of total NEAA and an increase of total EAA in Nnp fractions of both lupin species.
5

Changes in bioactive carbohydrates during dehydration processing on chickpeas

84%
Aguilera Y., Benitez V., Molla E., Lopez-Andreu F. J., Esteban R. M., Martin-Cabrejas M. A.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
6

Differences in accumulation of soluble alpha-galactosides during seed maturation of several Vicia species

84%
Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 2
Composition and levels of soluble α-galactosides: raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in developing seeds were measured by high resolution gas chromatography (HRGC) method. The studies were performed on maturing seeds of several wild and cultivated Vicia species: Viciaangustifolia L.(commonvetch), Vicia craccaL. (bird vetch), Vicia grandiflora Scop. (large yellow vetch), Vicia hirsuta (L.) S.F.Gray (tiny vetch), Vicia sativa L. (garden vetch, spring-growing cultivar Kwarta), and Vicia villosa Roth (winter vetch). In all Vicia species similar patterns in the accumulation of RF Os were observed. Galactinol - the donor of galactosyl moieties in α-galactosides biosynthesis was present in the middle stage of seed development, before appearing measurable levels of RFOs. Accumulation of RFOs started parallel with seed desiccation process. At first accumulation of the raffinose, then few days later stachyose and finally verbascose was noticed. In the final stage of seed maturation the verbascose was the main soluble α-galactoside (up to 3 % of dry weight, V. sativa). Besides the RFOs seeds of three Vicia species (V. cracca, V. hirsuta, and V. villosa) accumulated D-pinitol and its α-galactosides (Gal-C). Mono-galactosylpinitols (similar to raffinose) appeared in these species 2-4 days after galactinol, di-galactosyl pinitol A (common name: ciceritol) and di-galactosyl myo-inositol were present several days later than raffinose, and accumulation of tri-galactosyl pinitol A (TGPA) began after accumulation of stachyose. Matured seeds of V. hirsuta contained much more RFOs than Gal-C, opposite to seeds of V. villosa, and V. cracca where concentration of Gal-C was 4-8-fold higher than RFOs. In V. cracca seeds RFOs were almost replaced by Gal-C. In seeds of V. cracca and V. villosa the level of D-pinitol was significantly higher, than the level of myo-inositol. Contents of both cyclitols declined rapidly at the beginning of seed desiccation, when accumulation of RFOs and Gal-C quickly ini creased. We suggest that α-galactosides of D-pinitol can substitute raffinose family oligosaccharides and play similar role during seed maturation and storage.
7

Effects of diets with different contents of soybean alpha-galactosides and crude fibre on modification of duodenal microstructure and selected parameters of nutrient utilization in young turkeys

67%
Juskiewicz J., Jankowski J., Zdunczyk Z., Lecewicz A., Przybylska-Gornowicz B., Zieba M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 4
The aim of the present study was to assess the physiological response of growing turkeys’ duodenal surface to dietary replacement of a common dietary component – soybean meal (SBM) with a soy protein isolate (SPI), as this treatment was associated with almost complete removal of α-galactosides from the diet (from 2.44 to 0.15%). Additionally, the utilization of selected dietary ingredients upon dietary treatments was recorded. Effects of raffinose-family oligosaccharides were assessed as well in low- and high-fibre dietary environment (3.5 and 5.3% of crude fibre, respectively). This study revealed that the duodenal morphological parameters were differently affected by dietary treatments at different production stages, i.e. at 4 and 8 weeks of life. Although villus height/crypt depth ratio (VCR) was insignificantly decreased by high-α-galactoside treatment in younger 4-week birds, the presence of these oligosaccharides in the diet positively influenced the VCR index in 8-week turkeys. A similar tendency was observed when calcium retention was considered. Different contents of dietary crude fibre affected the physiological action of α-galactosides, including duodenal crypts depth and phosphorus retention in the 4-week birds, as well as duodenal goblet cells number and nitrogen utilization in the older turkeys. A high content of α-galactosides in the diet resulted in increased hydration of intestinal contents, but without a significant decline in the dry matter digestibility and utilization of nitrogen, calcium and phosphorus. Having in mind the development and physiology of the GIT, it may preliminary be concluded that in later production stages, total withdrawal of soybean α-galactosides from turkeys’ diets does not seem to be nutritionally advisable.
8

Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides

67%
Villaluenga C. M., Wardenska M., Pilarski R., Bednarczyk M., Gulewicz K.
Folia Biologica
|
2004
|
tom 52
|
nr 3-4
9

Influence of oligosaccharide extracts from pea and lupin seeds on caecal fermentation in rats

67%
Juskiewicz J., Zdunczyk Z., Wroblewska M., Gulewicz K.
Journal of Animal and Feed Sciences
|
2003
|
tom 12
|
nr 2
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