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Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro

100%

Dreger M., Krajewska-Patan A., Gorska-Paukszta M., Pieszak M., Opala B., Gryszczynska A., Adamczak A., Mikolajczak P. M., Buchwald W.

Herba Polonica

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2012

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tom 58

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nr 4

Tussilago farfara L. (family Asteraceae) is a valuable medicinal plant that has been used as a cough suppressant and as an antibacterial and anti-inflammatory drug. Mucopolysac charides, flavonoids, sterols, phenolic acids and pyrrolizidine alkaloids (PAs) are the main active compounds of coltsfoot. Due to hepatotoxic properties of some pyrrolizidine alkaloids, raw materials that contain PAs should be monitored and determined. The aim of present work was to establish nodal cultures of Tussilago farfara and to determine the content of senecionine and senkirkine in plants propagated in in vitro conditions. Eleven clones of coltsfoot derived from Polish natural populations were established. Rhizome buds were used as explants for the initiation of in vitro cultures on MS (Murashige and Skooge) medium. Every six weeks the shots and leaves were collected and dried. The HPLC method was applied for the identification and determination of senecionine and senkirkine. Content of pyrrolizidine alkaloids varied significantly depending on origin (population). An average sum of alkaloids (senecionine and senkirkine) ranged from 1.23 to 10.47 mg/100g d.w. that corresponds to 0.0013–0.011%, respectively.

2

Effect of nitrogen level in the substrate on yield and alkaloid content in Catharanthus roseus L. [G] Don.

100%

Lata B., Sadowska A.

Folia Horticulturae

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1996

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tom 08

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nr 2

3

Content of alkaloids and flavonoids in celandine (Chelidonium majus L.) herb at the selected developmental phases

84%

Seidler-Lozykowska K., Kedzia B., Bocianowski J., Gryszczynska A., Lowicki Z., Opala B., Pietrowiak A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2016

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tom 15

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nr 4

The content of alkaloids and flavonoids and the yield of herb were analyzed in greater celandine cultivar ‘Cynober’ during six following phases: spring rosette formation, the beginning of flowering, full bloom, green fruit, seed harvest, fall rosette formation. Yield of celandine herb was different at the investigated phases and in years of cultivation. The highest yield of herb was observed at the beginning of flowering, then a decrease was noticed, up to the phase of seed harvest, when the yield grown up. The lowest yield of celandine herb was obtained in last phase – fall rosette formation, except 2011 when the lowest yield was in spring rosette formation. The average content of alkaloids was the highest in phase of green fruit (1.097%), while in 2012, the highest content was reached in phase of fall rosette formation – 1.200%. The lowest content of alkaloids was obtained in herb of the beginning of flowering (0.608%) in both years. The mean content of flavonoids was from 0.310% (the beginning of flowering) to 0.522% (seed harvest) and was the same in both years. The stable high content of total alkaloids and flavonoids and individual alkaloids was noticed in phase of fall rosette. Our results suggest that seed maturity is the best time for celandine herb harvest regarding the herb yield and content of alkaloids and flavonoids.

4

Observation on the influence of spraying potatoes with lupine extract on the feeding and development of potato-beetle population (Leptinotarsa decemlineata Say.)

84%

Krzymanska J., Waligora D., Michalski Z., Peretiatkowicz M., Gulewicz K.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 1-3

5

The influence of alkaloids in seeds of Lupinus angustifolius on the growth and some meat features of ducks

84%

Karasinski D., Bednarczyk M., Peretiakowicz M., Gulewicz K.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 10-12

6

Distribution of atropine and scopolamine in different organs and stages of development in Datura stramonium L. [Solanaceae]. Structure and ultrastructure of biosynthesizing cells

84%

Iranbakhsh A., Oshaghi M. A., Majd A.

Acta Biologica Cracoviensia. Series Botanica

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2006

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tom 48

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nr 1

This paper reports a study of the alkaloid content of different parts of Datura stramonium L. (Solanaceae) in various stages of its growth and development, and the location, structure and ultrastructure of alkaloid-biosynthesizing cells (idioblasts) in different parts of naturally grown and cultured plant material. TLC, HPLC, and GC/MS methods were used for alkaloid assays. The results showed that alkaloid production starts from the end of the second week after seed germination, increases in different organs up to the tenth week of growth, and then decreases. Leaves and capsules showed the highest alkaloid content in the vegetative and generative stages, respectively. In leaves the alkaloids decreased rapidly in the generative stage. The highest alkaloid content was recorded in vegetative leaves, followed in descending order by vegetative petioles, generative and vegetative stems, generative petioles, generative roots, generative leaves, vegetative roots and mature seeds. The organs as well as calli derived from different leaf parts were examined for the presence of idioblasts by microscopic and cytochemical methods. Idioblasts were present only in semi-hyaline callus originated from leaf base; they were spherical or oval, with a thick cell wall and large central vacuole. These observations should prove helpful in attempts to produce specific alkaloids in naturally grown plants and cell cultures.

7

O możliwości zastosowania nasion łubinu żółtego (Lupinus luteus L.) jako surogatu kawy

67%

Piechocka J.

Roczniki Państwowego Zakładu Higieny

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1962

|

tom 13

|

nr 1

8

Evaluation of changes in the growth and chemical constituents of Anoectochilus formosanus Hayata grown under hydroponic conditions

67%

Nguyen H. C., Nhu T. Q. M., Dung P. V., Hieu N. D., Tuan T. T., Huyen P. X., Truong D.-H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

|

tom 99

|

nr 4

9

Poszukiwanie związków alkaloidowych w nasionach Cucurbita pepo L. i C. maxima DUCH. metodą chromatografi bibułowej

67%

Wojciechowska B.

Acta Biologica Silesiana

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1990

|

tom 14

10

Domestication and breeding improvement of narrow-leaved lupin [L.angustifolius L.]

67%

Swiecicki W., Swiecicki W. K.

Journal of Applied Genetics

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1995

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tom 36

|

nr 2

A great progress had been made in narrow-leafed lupin in a comparatively short period. Despite elimination of basic disadvantages (hard seeds, high content of alkaloids, pod shattering) disease-resistant, unbranched and thermoneutral cultivars have been bred. Investigations on the variation range and inheritance mode of commercially important characters were followed by breeding achievements. The paper contains a review of references for 35 genes controlling alkaloid content, growth rhythm, branching, vegetation length, shape and color of leaves, color of flowers and seeds, seed hardiness, pod characters and disease resistance. Authors of characters and gene symbols from different research centres and from different years are given on the basis of priority. The importance of these genes for breeding improvement is discussed. Type lines for characters/genes and genotype of more important cultivars collected in the Polish narrow-leafed lupin genebank are presented.

11

Yield of a herb [Datura innoxia Mill.] and content of alkaloids depending on development stage of harvested plants

67%

Buczkowska H., Pakula I.

Herba Polonica

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2007

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tom 53

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nr 3

The experiments were carried out in 2004-2006 in the Department of Vegetable and Medicinal Plants, Agricultural University in Lublin. The aim of presented work was to evaluate a development stage in which should the Datura innoxia plants be harvested to obtain herb with high content of alkaloids per area unit. Plants were harvested: 1 - in a stage of vegetative growth, 2 - in the beginning of flowering, 3 - in the beginning of fructification. The effects of weather conditions on air-dry herb yield (17.0-23.2 kg-100 m~2) and content of tropane alkaloids (0.166-0.224%) was stated. Significantly lower mean herb yield was obtained from plants harvested in vegetative growth stage. Significant relationship between tropane alkaloids content and development stage of plants was not stated. Quantity of tropane alkaloids obtained in cultivation of Datura innoxia (22.32-55.57 gl 00 nr2) depended more on high value of air-dry herb yield per area unit (12.9-28.5 kg-100 m2) than on content of these active substances in material (0.150-0.236%).

12

Wstępne badania nad optymalizacją warunków indukowania mutacji dla uzyskania nowej zmienności u maku (Papaver somniferum L.)

51%

Walkowiak M., Ogrodowczyk M.

Rośliny Oleiste - Oilseed Crops

|

2013

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tom 34

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nr 2

Celem prowadzonych badań było znalezienie optymalnych metod indukowania mutagenezy dla uzyskania nowej zmienności głównych alkaloidów maku: morfiny, tebainy i kodeiny. Materiał do badań stanowiły genotypy linii hodowlanych pochodzące z rekombinantów różnych form maku wysokomorfinowego z kolekcji IHAR-PIB oraz wysokomorfinowa odmiana Lazur. Badania prowadzono w trzech sezonach wegetacyjnych. Zastosowano czternaście sposobów indukowania mutagenezy stosując zróżnicowane stężenia mutagenu chemicznego i ultradźwięków oraz różny czas ekspozycji mutagenem. Nasiona traktowano metanosulfonianem etylu (EMS) oraz ultradźwiękami (UD) w celu ułatwienia wnikania substancji mutagennej do nasion. Stężenia mutagenu wynosiły: 0,4; 0,6; 0,8; 1,0; 1,2; 1,4 i 1,6% EMS. Nasiona wytypowanych genotypów maku pozostawały w roztworze trzy lub cztery godziny w zależności od stężenia mutagenu. Przeprowadzono również badania wpływu ultradźwięków na zwiększenie działania mutagenezy chemicznej na nasiona maku odmiany Lazur. Czas ekspozycji nasion na działanie ultradźwięków wynosił 5 lub 25 minut. Po zdekantowaniu roztworu nasiona wymywano pod bieżącą wodą i osączano na bibule. Nasiona poddane mutagenezie i linie kontrolne rozmnażano w szkółkach selekcyjnych. Zastosowanie mutagenezy pozwoliło na uzyskanie zmienności zawartości morfiny, kodeiny i tebainy. Poszerzenie zmienności zawartości alkaloidów w makowinach maku potwierdza, że EMS jest wydajnym mutagenem chemicznym stosowanym do tworzenia nowych zmienności.

Ograniczanie wyników

Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro

Effect of nitrogen level in the substrate on yield and alkaloid content in Catharanthus roseus L. [G] Don.

Content of alkaloids and flavonoids in celandine (Chelidonium majus L.) herb at the selected developmental phases

Observation on the influence of spraying potatoes with lupine extract on the feeding and development of potato-beetle population (Leptinotarsa decemlineata Say.)

The influence of alkaloids in seeds of Lupinus angustifolius on the growth and some meat features of ducks

Distribution of atropine and scopolamine in different organs and stages of development in Datura stramonium L. [Solanaceae]. Structure and ultrastructure of biosynthesizing cells

O możliwości zastosowania nasion łubinu żółtego (Lupinus luteus L.) jako surogatu kawy

Evaluation of changes in the growth and chemical constituents of Anoectochilus formosanus Hayata grown under hydroponic conditions

Poszukiwanie związków alkaloidowych w nasionach Cucurbita pepo L. i C. maxima DUCH. metodą chromatografi bibułowej

Domestication and breeding improvement of narrow-leaved lupin [L.angustifolius L.]

Yield of a herb [Datura innoxia Mill.] and content of alkaloids depending on development stage of harvested plants

Wstępne badania nad optymalizacją warunków indukowania mutacji dla uzyskania nowej zmienności u maku (Papaver somniferum L.)