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Previous studies have indicated that there is a cardiodepressant factor in the medium incubating the posterior pituitary lobe in situ. The cardiodepressant activity of the medium incubating the posterior pituitary lobe before and during stimulation of the vagus nerves was tested on isolated auricles of the right heart atrium of a two-day-old rat. It was found that the medium incubating the posterior pituitary lobe collected before stimulation decreased the contraction rate of the auricle by 34%, while that collected during the intermittent stimulation of the central ends of the cut vagus nerves caused a decrease of the auricle contractions frequency by 52%. The addition of cholinergic, serotoninergic, histaminergic receptor blockers or prostaglandin synthetase into Ringer-Lock’s solution bathing the auricle has no effect on the changes of the contraction rate caused by the incubation medium.
The experiment was performed on sexually mature female dogs. Operational procedures were performed according to classical surgical methods in general anaesthesia. The animals were kept alive for 21 days and subsequently put down and sectioned for the following material: the brain stem, the spinal cord with all the spinal ganglia, all the ganglia of the sympathetic trunk and the ganglia and plexuses of abdominal and pelvic cavities. The material was fixed in histological slides. The localization of retrograde changes, presented here, allows certification that the autonomic and afferent nerve fibres supplying the uterine cervix in the female dog originate from the central, as well as, from the peripheral autonomic nervous system.
The study was conducted on sexually mature female dogs. The extirpation of the different parts (both homs and body) of the uterus was performed. After 21 days, the following material for examination was collected: the brain stem, the thoraco-lumbo-sacral part of the spinal cord with its all spinal ganglia, sympathetic chain and also the ganglia and nerve plexuses of the abdominal and pelvic cavities. The experiments produced retrograde changes in nerve cells of the central and peripheral nervous system in the experimental animals. The comparison of the localization of retrograde changes in the neurons of the experimental animals and those of the control animal provided the basis for tracing the localization of the nerve cells which are the source of the nerve fibres supplying the horns and body of the uterus. The observations performed establish that these fibres are of multisource origin.
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