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Scaligeria meifolia Boiss., belonging to the Apiaceae family, grows wild in Iran. The essential oil from aerial parts of S. meifolia were obtained by hydrodistillation and analyzed by GC and GC/MS. Thirty-one constituents representing 99.98% of total oil components were identified. The main constituents of the essential oil were germacrene–D, (24.2%), germacrene-B (14.8%), limonene (14.2%), γ-elemene (11.6 %) and β-elemene (5.2%).
The present investigation was carried out to analyse the presence of endophytes in the above-ground parts of Hipericum perforatum and to analyse the biodiversity and enumeration of epiphytes. Plant material was collected in Poland three times during the growing season. Phenotypic and genotypic diversity of all the endophytes and the most abundant epiphytes were researched. We analysed fungistatic activity of this isolates. From the endosphere of tested plant Alcaligenes faecalis and Bacillus licheniformis were isolated. The most numerous epiphytes were the copiotrophs and a bit less numerous were oligotrophs, bacteria cultivated on Bunt and Rovir’s medium and fungi. The least numerous bacteria were Azotobacter sp. Among all the molds dominant were: Cladosporium herbarum, C. cladosporioides and Alternaria consortialis, A. alternata, Clonostachys rosea f. catenulata (Gliocladium catenulatum), Scopulariopsis brevicaulis and Penicillium terrestre. Among phyllobacteria there were found mostly the following species: Burkholderia cepacia, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas fluorescens, P. putida, Pantoea agglomerans, Paenibacillus polymyxa, Bacillus cereus, Rhodococcus sp., R. erythropolis and Cellulosimicrobium cellulans. The broadest spectrum of antifungal activity was examined for the following species: Paenibacillus polymyxa, Pseudomonas putida and Pantoea agglomerans. P. polymyxa limited the growth of over 82% tested molds, so did the other two strains: P. agglomerans over 77% and P. putida over 73%.
Artemisia aucheri Boiss. is a shrub from Asteraceae family has been widespread in Iran. In traditional medicine, A. aucheri is used for its astringent, disinfectant, antimicrobial and antiparasitic properties. The aim of this study was to evaluate the chemical composition and antimicrobial activity of essential oil from aerial parts of A. aucheri against different microorganisms including Gram positive, Gram negative bacteria, filamentous fungi and yeast by disc diffusion and micro broth dilution assays. The antioxidant activity of A. aucheri essential oil was evaluated by DPPH free radical scavenging system. Fifty five components were identified by GC and GC/MS analysis and quantified from the essential oil of A. aucheri, representing 98% of total oil. The major components were geranyl acetate (17.2%), E-citral (17.1%), linalool (12.7%), geraniol (10.7%), Z-citral (10.5%). The antimicrobial results showed that Pseudomonas aeruginosa was resistant to the oil and Staphylococcus aureus and Candida albicans showed the best sensitivity to the oil. The A. aucheri has powerful antioxidant activity than that of Trolox. Some investigations were be done for evaluating the efficacy of essential oil.
The total saponins isolated from aerial parts and roots of alfalfa (Medicago sativa L.) at the concentration of 0.01 %, 0.05% and 0.1 % showed differential influence on the mycelium growth of Alternaria zinniae, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Phoma poolensis and Rhizoctonia solani. A higher inhibitory effect on ill vitro growth of mycelium of all tested pathogens indicated saponins from roots of alfalfa than from aerial parts. Tested saponins from roots at the concentration of 0.1 % totally inhibited linear growth of Phoma narcissi, and linear growth of Alternaria zinniae was limited about 67%, Botrytis cinerea about 74%, Botrytis tulipae about 68%, Phoma poolensis about 38%, and Rhizoctonia solani about 74% in comparison to the control. The saponins of alfalfa from roots at the concentration of 0.1 % and 0.2% applied as preinoculation sprays evidently inhibited the development of Phoma narcissi on Hippeastrum leaves. This dose of aerial saponins of alfalfa did not effect the development of necrotic spots on the leaves.
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