Deaminations of adenine and adenosine by pattern strains of 24 staphylococcal species, were tested. During 3 hours of incubation of the suspensions of 8 staphylococci with adenine the liberation of ammonia occurred. The same staphylococci accumulated ammonia in the incubation medium with adenosine. The Staphylococcus intermedius PCM 2405 strain as opposite to the Staphylococcia aureus 536 strain in the media with adenine or adenosine accumulated hypoxanthine or inosine, respectively and ammonia. These results indicated that adenine deaminase (adenase) and adenosine deaminase activities were associated with the cells of the Staphylococcus intermedius PCM 2405 strain. Staphylococci were heterogenous within three species groups with respect to adenine and adenosine deaminations. Adenine and adenosine deaminations were absent in staphylococci belonging to the Staphylococcus simulans species group.
A pot experiment was carried out to study the effect of adenine applied at following rates: Q, 1,10 and 100 mg per 1 kg of typical brown soil on yield and chemical composition of carrot and on numbers of soil microorganisms as well as on activity of soil enzymes. Experiment was done in two series: with or without Azotobacter sp. inoculation. The favourable effect of applied adenine together with Azotobacter sp. on yield of carrot roots was observed. Adenine increased nitrogen and potassium uptake by carrot but adversely affected number of oligotrophic, macrotrophic, fixing of mineral nitrogen bacteria, ammonifers, Azotobacter sp. and actinomycetes. The inhibition action of this preparation on soil dehydrogenases, urease, and basic phosphatase and stimulation action on acid phosphatase have been found.