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1

Light-induced changes of actin architecture in plant cells and their role in chloroplast movements

100%
Krzeszowiec W., Dobrucki J., Gabrys H.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2

The cytotoxic effect of diphtheria toxin on the actin cytoskeleton

75%
Varol B., Bektas M., Nurten R., Bermek E.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
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nr 1
Diphtheria toxin (DT) and its N-terminal fragment A (FA) catalyse the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) into a covalent linkage with eukaryotic elongation factor 2 (eEF2). DT-induced cytotoxicity is versatile, and it includes DNA cleavage and the depolymerisation of actin filaments. The inhibition of the ADP-ribosyltransferase (ADPrT) activity of FA did not affect the deoxyribonuclease activity of FA or its interaction with actin. The toxin entry rate into cells (HUVEC) was determined by measuring the ADP-ribosyltransferase activity. DT uptake was nearly 80% after 30 min. The efficiency was determined as Km = 2.2 nM; Vmax = 0.25 pmol.min−1. The nuclease activity was tested with hyperchromicity experiments, and it was concluded that G-actin has an inhibitory effect on DT nuclease activity. In thepresence of DT and mutant of diphtheria toxin (CRM197), F-actin depolymerisation was determined with gel filtration, WB and fluorescence techniques. In the presence of DT and CRM197, 60–65% F-actin depolymerisation was observed. An in vitro FA-actin interaction and F-actin depolymerisation were reported in our previous paper. The present study thus confirms the depolymerisation of actin cytoskeleton in vivo.
3

Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential

75%
Nowak D., Skwarek-Maruszewska A., Zemanek-Zboch M., Malicka-Blaszkiewicz M.
Acta Biochimica Polonica
|
2005
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tom 52
|
nr 2
Human colon adenocarcinoma LS180 parental cell line and selected variants, characterized by different metastatic capacity were used to examine, whether a correlation exists between β-actin expression, its subcellular distribution and metastatic potential of these cells. Cytosolic fraction (supernatant 105 000 × g), isolated from the tumor cells was used as a source for actin quantification. The higher level of β-actin was observed in the cytosol of three selected sublines to compare with LS180 parental line. Statistically significant increase of β-actin level in highly motile EB3 cells variant should be underlined to compare with the other sublines. Distinct differences in the phenotype of adenocarcinoma cell variants were found, such as the changes in cells shape, cells spreading and ability to attach to the surface of culture dish. Actin cytoskeleton was visualized with fluorescence microscopy application and microfilaments rhodamine-conjugated phalloidin staining. β-actin subcellular localization was done by immunofluorescence staining with monoclonal anti-β actin antibodies. In the elongated cells (LS180, 3LNLN), this isoactin is dispersed in the whole cell body and concentrates in pseudopods and at the leading edges, when in the rounded variant (EB3) β-actin dominates mainly in cortical ring under cellular membrane and it is also seen in the subtle protrusions. Summary of our former (Nowak et al., 2002, Acta Biochim. Polon., 49: 823) and current data lead to the conclusion that there is a distinct correlation between metastatic capacity of examined human colon adenocarcinoma cells, the state of actin polymerization, actin cytoskeleton organization and β-actin expression.
4
Content available remote

Leptin decreases renal medullary Na plus, K plus-ATPase activity through phosphatidylinositol 3-kinase dependent mechanism

75%
Beltowski J., Marciniak A., Wojcicka G.
Journal of Physiology and Pharmacology
|
2004
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tom 55
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nr 2
We examined the effect of leptin on renal function and renal Na+,K+-ATPase and ouabain-sensitive H+,K+-ATPase activities in the rat. Leptin was infused under general anaesthesia into the abdominal aorta proximally to the renal arteries. Leptin infused at doses of 1 and 10 µg/kg/min increased urine output by 40% and 140%, respectively. Urinary Na+ excretion increased in rats receiving leptin at doses of 0.1, 1, and 10 µg/kg/min by 57.6%, 124.2% and 163.6%, respectively. Leptin had no effect on creatinine clearance, potassium excretion and phosphate excretion. Na+,K+-ATPase activity in the renal medulla of rats treated with 1 and 10 µg/kg/min leptin was lower than in control animals by 25.5% and 33.2%, respectively. In contrast, cortical Na+,K+-ATPase as well as either cortical or medullary ouabain-sensitive H+,K+-ATPase activities did not differ between leptin-treated and control animals. The effect of leptin on Na+,K+-ATPase activity was abolished by actin depolymerizing agents, cytochalazin D and latrunculin B, and by phosphatidylinositol 3-kinase (PI3K) inhibitors, wortmannin and LY294002. These results indicate that: 1) natriuretic effect of leptin is mediated, at least in part, by decrease in renal medullary Na+,K+-ATPase activity, 2) inhibition of medullary Na+,K+-ATPase by leptin is mediated by PI3K and requires integrity of actin cytoskeleton.
5

Light-induced in vivo reorganization of actin cytoskeleton in leaves of Nicotiana tabacum transformed with plastin-GFP

75%
Anielska-Mazur A., Dobrucki J., Timmers A., Gabrys H.
Polish Journal of Natural Sciences. Supplement
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2003
|
tom 01
6

Copper complex with superoxide dismutase-like activity induces a rise of actin filamentous structures

75%
Urbancikova M., Jantova S.
Folia Histochemica et Cytobiologica. Supplement
|
1997
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tom 35
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nr 2
7

Dynamin: characteristics, mechanism of action and function

63%
Wiejak J., Wyroba E.
Cellular and Molecular Biology Letters
|
2002
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tom 07
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nr 4
Dynamin - a member of the GTP-ase protein family - is essential for many intracellular membrane trafficking events in multiple endocytic processes. The unique biochemical features of dynamin - especially its propensity to assemble - enable severing the nascent vesicles from the membrane. The mechanism of dynamin’s action is still a subject of debate - whether it functions as a mechanochemical enzyme or a regulatory GTPase. The GTPase domain of dynamin contains three GTP-binding motifs. This domain is very conservative across the species, including that recently cloned by us in the unicellular eukaryote Paramecium. Dynamin interacts with a number of partners such as endophilin and proteins involved in coordination of endocytosis with motor molecules. A growing body of evidence indicates that dynamin and dynamin-related proteins are involved both in pathology and protection against human diseases. The most interesting are dynamin-like Mx proteins exhibiting antiviral activity.
8
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Content available

Stabilization of actin filaments with phalloidin has no influence on chloroplast movement in Arabidopsis mesophyll cells

63%
Krzeszowiec W., Jagiello-Flasinska D., Gabrys H.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
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tom 94
|
nr 3
9

The role of exportin 6 in cytoskeletal-mediated cell death and cell adhesion in human non-small-cell lung carcinoma cells following doxorubicin treatment

63%
Izdebska M., Gagat M., Grzanka D., Halas M., Grzanka A.
Folia Histochemica et Cytobiologica
|
2014
|
tom 52
|
nr 3
10

Influence of BCR-ABL fusion proteins on the course of Ph leukemias

63%
Telegeev G. D., Dubrovska A. N., Dybkov M. V., Maliuta S. S.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
The hallmark of chronic myeloid leukemia (CML) and a subset of acute lymphoblastic leukemia (ALL) is the presence of the Philadelphia chromosome as a result of the t(9;22) translocation. This gene rearrangement results in the production of a novel oncoprotein, BCR/ABL, a constitutively active tyrosine kinase. There is compelling evidence that the malignant transformation by BCR/ABL is critically de­pendent on its Abl tyrosine kinase activity. Also the bcr part of the hybrid gene takes part in realization of the malignant phenotype. We supposed that additional muta­tions accumulate in this region of the BCR/ABL oncogene during the development of the malignant blast crisis in CML patients. In ALL patients having p210 fusion pro­tein the mutations were supposed to be preexisting. Sequencing of PCR product of the BCR/ABL gene (Dbl, PH region) showed that along with single-nucleotide substitutions other mutations, mostly deletions, had oc­curred. In an ALL patient a deletion of the 5th exon was detected. The size of the de­letions varied from 36 to 220 amino acids. For one case of blast crisis of CML changes in the character of actin organization were observed. Taking into account the functional role of these domains in the cell an etiological role of such mutations on the disease phenotype and leukemia progression is plausible.
11

Blue light-induced orientation movements of chloroplasts in higher plants: Recent progress in the study of their mechanisms

63%
Gabrys H.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 4
12
Content available remote

Actin cytoskeleton and beta-actin expression in correlation with higher invasiveness of selected hepatoma Morris 5123 cells

51%
Popow A., Nowak D., Malicka-Blaszkiewicz M.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 7
111-123
Our studies were focused on the isolation and characterization of highly motile fraction of cells from hepatoma Morris 5123 population. Cells that underwent several migration cycles through Matrigel - coated filters were successfully cultured. The invasion index was determined by means of Matrigel invasion assay. Statically significant increase in the value of invasion factor for selected cells variant in comparison to the parental population was observed. The considerable changes in the cell shape were followed by the reorganization of the actin cytoskeleton structure including a dense subcortical congestion in the distribution of ß-actin isoform. The visualization of this protein in tumor cells was performed by immunostaining and scanning fluorescent confocal microscopy. The results were confirmed by densitometry analysis of Western blots. In addition, the increased state of actin polymerization in the cytoplasmic fraction of selected cells was determined as measured by filamentous to monomeric (F:G) actin ratio. Concluding, the selected fraction of hepatoma Morris 5123 cells with higher invasion capacity was characterized by rounded shape, remarkable increase of ß-actin level, its submembrane concentration as well as with the increased state of actin polymerization with respect to parental cells population.
13

Immunoreceptor signaling in innate and adaptive immune system cells

45%
Swat W.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 5
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