Wyniki wyszukiwania - AGRO

1

Ocena testow na patogennosc szczepow Yersinia enterocolitica izolowanych od ludzi i zwierzat

100%

Jakubczak A., Siemionek J., Anusz Z.

Medycyna Weterynaryjna

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1992

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tom 48

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nr 07

313-316

Pathogenicity of Yersinia enterocolitica in vitro was determined simultaneously by means of three tests: production of piramidaze at 30°C, autoagglutination at 37°C and inhibition of growth on MOX agar medium plate at 37°C. The strains were collected from diseased people, a dead chinchilla and from healthy pigs and foxes after their slaughter. The findings showed that the test for piramidase production proved to have better usefulness to distinguish pathogenic strains from apathogenic ones. Pigs in contrast to foxes were found to be the reservoir of Yersinia enterocolitica strains pathogenic for man.

2

Yersinia enterocolitica a zdrowie ludzi i zwierzat

100%

Jakubczak A.

Życie Weterynaryjne

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1992

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tom 67

|

nr 05

114-115

3

Dzialanie wybranych antybiotykow i srodkow dezynfekcyjnych na szczepy Yersinia enterocolitica izolowane od ludzi i swin

88%

Kot B., Bukowski K., Jakubczak A., Denka M., Luczka A.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

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2001

|

tom 60

17-27

4

Adhezja i tworzenie biofilmu przez rozne biotypy Yersinia enterocolitica

88%

Kot B., Jakubczak A., Dominska M., Kobylko M.

Medycyna Weterynaryjna

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2005

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tom 61

|

nr 07

800-803

5

Antagonistic activity of lactic acid bacteria on strains of Yersinia enterocolitica o:3 isolated from slaughtered pigs

88%

Jakubczak A., Kot B., Bukowski K.

Advances in Agricultural Sciences

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1996

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tom 05

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nr 1

31-35

6

The influence of environmental factors on survival of Yersinia enterocolitica 0:3

88%

Kot B., Blaszczyk M., Kulawiec U.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 3

The influence of the presence of bacteriophage ZD5, temperature, pH and the interaction of these factors on survival of Y. enterocolitica cells in pond water and buffers was examined. The presence of bacteriophage ZD5 and temperature have substantial influence on survival of Y. enterocolitica cells in buffers with different pHs. The statistical analysis showed a significant influence of the presence of phage ZD5 on the decrease in the number of Y. enterocolitica cells incubated in buffers with pHs 6, 7, and 8. In the presence of phage ZD5, pH itself turned out to be of no significance because the average numbers of Y. enterocolitica cells obtained in pHs 6, 7, and 8 did not differ significantly at p≤0.05. Statistical analysis confirms significant influence of the presence of phage ZD5 on the reduction of the number of Y. enterocolitica cells in pond water at both 4°C and 20°C. The lowest average value of the number of cells was obtained at 4°C in the presence of phage ZD5, both in the tested buffers and in the pond water.

7

Osmoregulation - dependent expression of Yersinia enterocolitica virulence factors

88%

Brzostek K., Stencel I., Hrebenda J.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 1

8

Immunochemical studies on R mutants of Yersinia enterocolitica O:3

88%

Radziejewska-Lebrecht J., Skurnik M., Shashkov A. S., Brade L., Rozalski A., Bartodziejska B., Mayer H.

Acta Biochimica Polonica

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1998

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tom 45

|

nr 4

Three mutants of Yersinia enterocolitica O:3, namely: YeO3-R1, YeO3-RfbR7 and YeO3-c-trs8-R were classified on the basis of sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS/PAGE) profile of isolated lipopolysaccharides (LPS) as belonging to the Ra- (the first) and the Rc-type (the other two mutants). Methylation analysis, in addition to 13C and 1H NMR studies of purified core oligosaccharides revealed structures similar to those established previously for the full core of Y. enterocolitica O:3 in the case of the Ra mutant, and identical to that reported for the Rc mutant Ye75R, in the case of the two other mutants. The O-specific sugar, 6d-l-altrose, which forms a homopolymeric O-chain, was present in small amounts in all three LPS preparations, as well as in the core oligosaccharide preparations along with the Ra and the Rc sugars, characteristic of the Y. enterocolitica O:3 core. This result is in line with genetic data, indicating that it is the inner core region which is the receptor for the O-specific chain in Y. enterocolitica O:3. This region seems likewise to be the anchoring region for the enterobacterial common antigen (ECA), as shown by SDS/PAGE/Western blot analysis with monoclonal antibodies against ECA. In addition, we also demonstrated that the Ye75R mutant Rc and its parental strain Ye75S, both were ECA-immunogenic strains. So far, ECA-immunogenic strains, i.e. those with LPS-linked ECA, were only identified in E. coli mutants of the R1, R4 and K-12 serotype.

9

Przezywalnosc Yersinia enterocolitica w miesie wieprzowym w zaleznosci od wielkosci inoculum i temperatury zamrazania

75%

Jakubczak A., Maleszewski J.

Medycyna Weterynaryjna

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1992

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tom 48

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nr 10

466-467

The objective of the studies was to evaluate survival of Yersinia enterocolitica serotypes 0:3 and 0:9 in freezed pork meat. After 42 days storage of meat samples at —10 and — 20°C live cells of the bacterium were found when an inoculum contained 10⁴ cells of Y. enterocolitica/g. Y. enterocolitica was not found after 28 days of storage of meat at — 10°C and after 35 days of storage at — 20°C when an inoculum contained 10³ of bacterial cells/g. The results point to a better survival of Y. enterocolitica in pork meat stored at — 20°C than in — 10°C.

10

The activity of chosen bacteriophages on Yersinia enterocolitica strains

75%

Kot B., Bukowski K., Jakubczak A., Kaczorek I.

Polish Journal of Veterinary Sciences

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2002

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tom 05

|

nr 1

11

Fenotypowa i genotypowa analiza wlasciwosci chorobotworczych szczepow Yersinia pseudotuberculosis otaz Yersinia enterocolitica

75%

Gackowska I., Furowicz A. J., Czernomysy-Furowicz D., Jakubczak A.

Medycyna Weterynaryjna

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2002

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tom 58

|

nr 04

303-305

12

Adhezja szczepow Yersinia enterocolitica izolowanych od ludzi i swin do folii kolagenowej i skory

75%

Kot B., Wozniak-Kosek A., Wolska K.

Medycyna Weterynaryjna

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2002

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tom 58

|

nr 12

985-988

13

Wpływ immunizacji świń zawiesiną wyselekcjonowanych szczepów Yersinia enterocolitica na przebieg doświadczalnego zakażenia oraz okres siewstwa drobnoustrojów

75%

Platt-Samoraj A., Szweda W., Procajlo Z., Mikulska-Skupien E., Bancerz-Kisiel A., Szczerba-Turek A., Syczylo K.

Medycyna Weterynaryjna

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2013

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tom 69

|

nr 09

Y. enterocolitica infection of pigs is concerned with a common carrier and shedding states in this species. The aim of the study was to determine the influence of the experimental immunization of pigs with a selected Y. enterocolitica strains suspension on the controled infection course, the antibody level formation, as well as the duration of pathogen shedding. The immunization of pigs was enforced with a suspension of Y. enterocolitica strains previously inactivated with formol, suspended in PBS and demonstrated in vitro high immunogenicity in Respiratory Burst Activity/Potential Killing Activity (RBA/PKA) and Mitogen Transformation Test (MTT). The study was performed on 15 pigs divided into 3 groups. The animals from groups I and II received an immunizate with a density of 2.7 × 10⁹ cfu/ml administered subcutaneously in doses 2 ml and 5 ml, respectively, twice in a 2-week interval. The third group (control) was administered PBS in an analogous scheme. The evaluation in vivo was conducted after a per os challenge with a pathogenic strain of Y. enterocolitica O:3. The first and booster immunization had no effect on the clinical picture and body weight gains of the immunized animals. The fastest and strongest immune response to the per os challenge with Y. enterocolitica O:3 was observed in the control group, already in the first week post infection (wpi). Higher antibody levels were found in the group of animals where 5 ml 2.7 × 10⁹ cfu/cm³ subcutaneously were administered. In the first wpi bacterial shedding was observed in all animals that belonged to group I and the control group which persisted up to 3 wpi. Among the pigs from group II the shedding was observed in 3 out of 5 animals, and which finished in 6 days post infection. Applied experimental immunization against per os challenge with a pathogenic strain of Y. enterocolitica O:3 did not prevent pathogen shedding, but merely limited its intensity and duration.

14

Mechanizmy regulacji czynnikow wirulencji Yersinia enterocolitica

75%

Brzostek K.

Postępy Mikrobiologii

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2004

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tom 43

|

nr 1

7-38

15

Izolacja wirulentnych szczepów Yersinia enterocolitica z migdałków świń

75%

Staroniewicz Z.

Medycyna Weterynaryjna

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1988

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tom 44

|

nr 09

Глоточные миндалины от 154 убойных свиней исследовались на наличие Y. enterocolitica. Изолировано 13 штаммов, из которых 9 заквалифицировано к серотипу 3, а 5 штаммов к серотипу 9. Изолированные штаммы усследовались на вируленцию. Применено 3 критерия для оценки вирулентности: автоагглютинация, патогенность для мышеи и заторможение роста на magnesium-oxalate agar в 37°С. Полученные результаты внушают, что свиньи являются натуральным резервуаром вирулентных штаммов Y. enterocolitica и могут являться потенциальным источником инфекции для других животных и человека.

16

Occurrence of Yersinia enterocolitica in canine excrements contaminating urban lawns

75%

Wojciechowska B., Mikulska-Skupien E., Platt-Samoraj A., Szczerba-Turek A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

|

nr 2

153-159

The purpose of the studies was to determine, by means of bacteriological and molecular methods, the occurrence of Y. enterocolitica in the faeces of dogs, as well as to assess the degree of their spread in the urban environment. The faeces were collected from the lawns of six large districts of Olsztyn. In order to isolate Y. enterocolitica, "warm culture" (ITC/CIN) and "cold culture" (PSB/CIN) were used in parallel, together with biochemical tests. Next, genomic DNA of Y. enterocolitica was isolated and αil, ystA, and ystB genes were detected with the use of the multiplex PCR method. A relatively frequent occurrence of Y. enterocolitica strains in canine faeces contaminating the urban lawns of Olsztyn was demonstrated. The greatest percentage of contaminated faecal samples was found in the area of large housing estates. By means of molecular tests, the presence of ystB gene only, in the absence of αil and ystA genes, was demonstrated in the Y enterocolitica genome, which could indicate a potentially pathogenic biotype 1A. Therefore, it seems important to keep monitoring the changes, which occur within this species of microorganisms, the epidemiological situation of yersiniosis in human and animal populations, as well as to continue the studies on the epidemiology of Y enterocolitica infections also in the context of a reservoir of animals accompanying a man.

17

Zjadliwosc Yersinia enterocolitica oraz znaczenie biotypu 1A w patogenezie jersiniozy

75%

Platt-Samoraj A., Bancerz-Kisiel A., Szweda W.

Medycyna Weterynaryjna

|

2006

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tom 62

|

nr 10

1113-1115

Yersinia enterocolitica is an extremely varied organism due to its biochemical, antigen and pathogenic properties. Six biotypes are distinguished within the species but, from the clinical point of view, only five of them: 1B, 2, 3, 4, and 5 are considered to be the cause of yersiniosis. The main criterion for the above division is pathogenicity, which correlates with presence of the virulent pYV plasmid. Microorganisms which lack this plasmid are considered to be a-virulent. However the pYV plasmid may easily be lost during the process of culturing or storage. Biotype 1A strains of Y. enterocolitica are often considered to be non-pathogenic primarily because they do not posses the pYV. These same strains of 1A biotype are able to cause diseases through unknown mechanisms that are independent of the virulent plasmid presence. Evaluating the pathogenicity of this biotype seems to be more complex. The increasing significance of 1A biotype and the possibility of loosing pYV in strains belonging to residual biotypes necessitate further studies which would clear up doubts relating to Y. enterocolitica pathogenicity.

18

Application of the suckling mouse bioassay to assess the enterotoxic properties of Yersinia enterocolitica strains isolated from fattening pigs

75%

Bancerz-Kisiel A., Szczerba-Turek A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

|

nr 1

Enterotoxic capacities of 95 Yersinia enterocolitica strains isolated from fattening pigs by the use of the suckling mouse bioassay were investigated. A positive result was received for 14 strains (14.74%), doubtful for 33 strains (34.74%), while the rest of 48 strains (50.52%) did not induce the production of enterotoxins. The received results show that in the pig population there are almost 15% of Yersinia enterocolitica strains, which are directly dangerous to public health because of their enterotoxic capacity. It is also worth paying attention to those strains, which in the suckling mouse bioassay provided doubtful results (around 35%) because their ability to produce enterotoxins was not explicitly declared as impossible.

19

Antibacterial activity of the preparation from Lactobacillus plantarum culture against Yersinia enterocolitica

75%

Laniewska-Moroz L., Warminska-Radyko I.

Polish Journal of Food and Nutrition Sciences

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1999

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tom 08

|

nr 1

31-36

The greatest antibacterial activity showed the preparation obtained from Lactobacillus plantarum after 48 h of culturing in MRS medium with glucose. The antibacterial preparation limited the growth of Yersinia enterocolitica by five orders of magnitude (to 103 cfu/mL) after 24 h of incubation with the preparation, as compared to the control sample with the cell number of 108 cfu/mL.

20

Wplyw temperatury na przyleganie do powierzchni stalych szczepow Yersinia enterocolitica izolowanych od ludzi i swin

75%

Kot B., Bukowski K., Pojnar J.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

|

2002

|

tom 62

121-128

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