The aim of the presented studies was to evaluate the usefulness of previously described method for the diagnosis of green mold in Polish mushroom-growing cellars. The PCR analysis showed the possibility of its use for distinguish Trichoderma aggressivum strains, occurring in mushroom crops. Molecular differences among green mold isolates were stated. They showed DNA band pattern characteristic for T. aggressivum f. europeanum as well as different pattern, suggesting the presence of strains belonging to T. aggressivum f. aggressivum. We successfully amplified purified DNA as well as DNA from purified cells suspension and cells collected on the casing surface while were not able to obtain PCR product from compost and casing suspensions.
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