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Increased production of carrageenase by Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design

100%

Ziayoddin M., Lalitha J., Shinde M.

International Letters of Natural Sciences

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2014

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tom 12

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nr 2

The culture conditions for the production of carrageenase were optimized using one-factor-at-a-time method combined with orthogonal array design. With the one-factor-at-a-time method revealed optimal conditions for carrageenase production were 24 h of fermentation period, 28 °C incubation temperature at pH 8.0 with NaNO3 as nitrogen source and carrageenan as carbon source in MMS media. Further optimization of carrgeenase production by using orthogonal experimental design L9 (34) with four factors, temperature, pH, NH4NO3 and carrageenan with their relevant levels revealed optimised conditions for carrageenase production were temperature of 28 °C, pH 8.0, 2 g L-1 NaNO3 and 2 g L-1 carrageenan. The order of the factors affecting the fermentation process was found to be temperature > pH > NaNO3 > carrageenan. The temperature played a significant role on the carrageenase production. Higher carrageenase yield with activity of 0.542 ±0.045 U ml-1 was obtained in the optimised medium when compared to those of basal medium. Carrageenase hydrolysed products of carrageenan were identified by LC-ESI-MS as neocarrabiose, neocarrabiose-4 sulfate, neocarratetraose, neocarratetraose-4 sulfate, anhydrogalactose, galactose, galactose-4 sulphate and sulphate.

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Optimal conditions for levan biopolymer production and its use in the synthesis of bactericidal levan-ZnO nanocomposite

75%

Taran M., Lotfi M., Safaei M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 4

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Optimization of agarase production by alkaline Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design

75%

Ziayoddin M., Lalitha J., Shinde M.

International Letters of Natural Sciences

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2014

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tom 12

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nr 2

The culture conditions for the production of extracellular agarase by Pseudomonas aeruginosa ZSL-2 were optimized using One-Factor-At-A-Time combined with orthogonal array design. One-Factor-At-A-Time method investigates the effect of time, temperature, NaCl, carbon sources, nitrogen sources and pH on agarase production. The optimized culture conditions obtained from the statistical analysis were temperature of 30 °C, pH 8.5, NH4NO3 2 g L-1 and agar 3 g L-1. The L9 orthogonal array design was used to select the fermentation parameters influencing the yield of agarase. The order of the factors affecting the fermentation process was found to be NH4NO3 > pH > agar > temperature, with temperature playing a significant role on the agarase production (p < 0.10). The higher yields than those in basal media culture were obtained in the final optimized medium with activity of 0.439 ± 0.013 U ml-1. Extracellular agarase hydrolysed agar into a range of oligosaccharides which were analysed by LC-ESI-MS spectrometry as anhydrogalactose, galactose, agarobiose, agarotetrose and agarohexaose.

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Analysis of the influence of cutting parameters on surface roughness of milled wood based on Taguchi techniques

63%

Wilkowski J., Rousek M., Svoboda E., Kopecky Z., Czarniak P.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2013

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tom 84

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Taguchi analysis of cutting power and thrust force in drilling of polyethylene bonded particleboards

63%

Wilkowski J., Borysiuk P., Czarniak P., Rousek M.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2012

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tom 80

6

Narzędzia TQM stosowane w polskich przedsiębiorstwach

32%

Karaszewski R.

Problemy Jakości

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1999

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tom 31

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nr 07

Ograniczanie wyników

2 Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

2 International Letters of Natural Sciences

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Problemy Jakości

2 Czarniak P.

2 Lalitha J.

2 Rousek M.

2 Shinde M.

2 Wilkowski J.

2 Ziayoddin M.

1 Borysiuk P.

1 Karaszewski R.

1 Kopecky Z.

1 Lotfi M.

1 Safaei M.

1 Svoboda E.

1 Taran M.

1 2019

2 2014

1 2013