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1

Halotolerance phenomena in Synechocystis sp. from the North Sea

100%
Mishra S., Krumbein W. E., Palinska K. A.
Oceanological Studies
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2001
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tom 30
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nr 1-2
2

The small heat shock proteins in plants are members of an ancient family of heat induced proteins

100%
Vierling E.
Acta Physiologiae Plantarum
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1997
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tom 19
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nr 4
In response to high temperature stress, plants express numerous small heat shock proteins (sHSPs) belonging to at least five related gene families. in vitro studies suggest sHSPs act as molecular chaperones to prevent irreversible heat denaturation of other proteins. The diversity of sHSPs in plants is unique among eukaryotes and makes it of interest to understand the origins of these proteins. sHSP-related proteins have now been identified in 13 prokaryotes, and in many of these prokaryotes the sHSPs are heat-regulated as seen higher plants. The prokaryotic sHSPs were analyzed by pairwise and mutliple sequence alignments with each other and with plant sHSPs. The higher plant class I cytosolic sHSPs are shown to be most similar to a subset of the prokaryotic sHSPs, including HSP 16.6 from the cyanobacterium Synechocystis. Genetic studies in this model cyanobacterium may provide insight into sHSP function in vivo, and into potential roles of sHSPs in higher plant cells.
3
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Morphological and physiological characterization of the delta-psaL mutant of cyanobacterium Synechocystis PCC6803

80%
Klodawska K., Kis M., Malec P., Kovacs L., Gombos Z., Strzalka K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
4

Detection and identification of potentially toxic cyanobacteria in Polish water bodies

61%
Glowacka J., Szefel-Markowska M., Waleron M., Lojkowska E., Waleron K.
Acta Biochimica Polonica
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2011
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tom 58
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nr 3
The main goal of this study was to determine the distribution of potentially toxic cyanobacteria in 39 selected Polish water bodies. From the water bodies with blooms and also from those in which blooms were not visible 87 samples were investigated. For the first time samples from ponds localized in villages with high agricultural activities were included. Lakes for which microcystin concentrations had been determined before were included as a reference for the research. The detection of cyanobacteria was conducted by microscopic observation as well as by PCR amplification of the rpoC1 gene fragment. Cyanobacteria were present in 75 out of 87 samples. The presence of potentially toxic cyanobacteria was detected by amplification of the mcyB and mcyE genes, which are involved in the biosynthesis of microcystins. Both genes were detected in 7 out of 9 blooms investigated. In the case of samples collected from water bodies in which blooms were not observed, the mcyB and mcyE genes were detected in 20 out of 36. In order to identify the cyanobacteria occurring in selected reservoirs, 16S plus ITS clone libraries were constructed. The method allowed distinguishing 18 different genotypes. After sequence analysis, cyanobacteria belonging to genera Microcystis, Planktothrix, Anabaena, Pseudanabaena, Synechocystis, Synechococcus and Woronichinia were identified. Results confirmed the usefulness of the rpoC1 and mcy genes for monitoring water bodies and detection of potentially toxic cyanobacteria. Application of molecular markers allowed detecting potentially toxic cyanobacteria before the bloom was visible. This is the first comprehensive study concerning cyanobacteria present in different types of Polish water bodies performed using molecular markers.
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