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  1. 6 Polish Journal of Microbiology

  2. 4 Medycyna Doświadczalna i Mikrobiologia

  3. 4 Postępy Mikrobiologii

  4. 3 Acta Microbiologica Polonica

  5. 3 Archivum Immunologiae et Therapiae Experimentalis

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  1. 3 Gospodarek E.

  2. 2 Bialecka A.

  3. 2 Biedron R.

  4. 2 Galkowska H.

  5. 2 Kasprowicz A.

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  1. 4 2018

  2. 1 2015

  3. 1 2014

  4. 2 2013

  5. 1 2012

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1

Influence of air-ions on selected microorganisms

100%
Wiszniewski A., Szczygiel M., Hudy S.
Acta Microbiologica Polonica
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2003
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tom 52
|
nr 2
2

L-forms of Staphylococcus epidermidis indeced by penicillin

88%
Wroblewska J., Janicka G., Gospodarek E., Szymankiewicz M.
Polish Journal of Microbiology
|
2006
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tom 55
|
nr 3
L-forms of S. epidermidis were induced at 35°C with the use of an L-form medium with penicillin. The aim of this study was to evaluate the frequency of L-form induction and demonstrate whether the origin of the clinical strains affects the frequency of L-forms induction, as well as to study whether the time of action of the antibiotic has an influence on frequency of L-form induction.
3

Biological evaluation of quaternary bis ammonium salt and cetylpyridinum bromide against S. epidermidis biofilm

75%
Stefanska J., Pietruczuk-Padzik A., Struga M., Borkowski M., Tyski S.
Polish Journal of Microbiology
|
2013
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tom 62
|
nr 4
Quaternary ammonium compounds are broad-spectrum bacteriocides widely used as antiseptics, disinfection and preservation agents. The aim of this study was to examine the activity of two quaternary ammonium salts, cetylpyridinum bromide and a newly synthesized quaternary bis ammonium salt, against S. epidermidis biofilm. The average values of killing efficiency for cetylpyridinum bromide ranged from 26.6% to 64.1% for all tested concentrations (0.125 to 8.0 μg×mL⁻¹) and for quaternary bis ammonium salt the percentage of killing efficiency ranged from 59.7% to 88.4% for tested concentrations (from 2.0 to 128.0 μg×mL⁻¹). Both tested compounds significantly affect staphylococcal biofilms, but any of used concentrations caused a total eradication of bacterial biofilm.
4

Porównanie trzech metod oceny wytwarzania śluzu przez Staphylococcus aureus i Staphylococcus epidermidis

75%
Wroblewska J., Ciok–Pater E., Sekowska A., Gospodarek E.
Medycyna Doświadczalna i Mikrobiologia
|
2010
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tom 62
|
nr 4
Oceniono zdolność wytwarzania śluzu przez 32 szczepy Staphylococcus aureus і 32 szczepy Staphylococcus epidermidis wykorzystując metody: ocenę morfologii kolonii na podłożu z czerwienią Kongo (CRA), z zastosowaniem roztworu safraniny (CT) i z użyciem metody spektrofotometrycznej z zastosowaniem czerwienieni Kongo (SC). Nie wykazano istotnych różnic w ocenie wytwarzania śluzu przez S. aureus i S. epidermidis przy wykorzystaniu metody CT i SC (p > 0,05), w przeciwieństwie do metody CRA (p < 0,0004 i p < 0,00001).
5

Identification and study of the behavior of S. aureus and S. epidermidis in fresh and frozen strawberries

75%
Steinka I., Kukulowicz A.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2018
|
tom 17
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nr 1
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Characterization of Staphylococcus epidermidis isolates cultured from patients with infected hip prostheses

75%
Bogut A., Niedzwiadek J., Strzelec-Nowak D., Plewik D., Mazurkiewicz T., Marczynski W., Olender A.
Health Problems of Civilization
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2018
|
tom 12
|
nr 2
Background. Coagulase negative staphylococci are at the forefront of etiologic agents of periprosthetic joint infections (PJIs). The purpose of the study was to characterise causative isolates (n=19) of Staphylococcus epidermidis (SE) – with emphasis on their phenotypic and genotypic heterogeneity. Material and methods. The isolates were cultured from multiple samples obtained perioperatively during revision surgery from 14 patients with clinically and/or microbiologically proven PJI. Phenotypic heterogeneity included variations of colony morphologies, drug resistance patterns and/or the capability of the biofilm formation and was verified by the DNA fingerprinting assay. Results. Phenotypic discrepancies were observed between isolates cultured from 5 patients (35.7%). The genotyping assay identified 3 pairs of isolates as unrelated; single pairs were genetically related and indistinguishable. The biofilm production was detected in 17 isolates, among which 5 (29.4%) were proficient biofilm formers harbouring the icaADBC genes. Additionally, one ica-positive isolate produced a moderate, protease-sensitive biofilm. The remaining isolates were moderate biofilm producers among which four developed protease-sensitive biofilms. Conclusions. The majority of PJIs are monoclonal; nevertheless, phenotypic diversity of SE is a frequent phenomenon which can complicate the diagnostic proceeding. Adherence ability is an important pathogenic trait of SE although the chemical composition of the resultant biofilm, its intensity and regulation of development can vary.
7
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Content available

EDTA as a potential agent preventing formation of Staphylococcus epidermidis biofilm on polichloride vinyl biomaterials

75%
Juda M., Paprota K., Jaloza D., Malm A., Rybojad P., Gozdziuk K.
Annals of Agricultural and Environmental Medicine
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2008
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tom 15
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nr 2
8

Enterotoksyny gronkowcowe w żywności

63%
Sciezynska H., Mackiw E., Pawlowska K., Modzelewska M.
Przemysł Spożywczy
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2013
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tom 67
|
nr 10
Zatrucia pokarmowe u ludzi mogą być związane z obecnością enterotoksyn gronkowcowych w żywności. W 2011 r. w krajach Unii Europejskiej stwierdzono wzrost zachorowań wywołanych spożyciem żywności zanieczyszczonej enterotoksynami gronkowcowymi. Najwięcej zatruć pokarmowych wywołanych przez enterotoksyny gronkowcowe związanych było z mieszaną żywnością (40%) oraz produktami piekarskimi (11,4%). Obecnie opisano 21 typów serologicznych enterotoksyn, oznaczonych literami od A doV.
9

Dwa oblicza bakterii Staphylococcus epidermidis, czyli od komensala do patogenu

63%
Podgorska B., Kedzia D.
Postępy Mikrobiologii
|
2018
|
tom 57
|
nr 4
10

Charakterystyka molekularna szczepow Staphylococcus aureus i Staphylococcus epidermidis wyizolowanych z materialu klinicznego

63%
Zdzalik D., Dominiak A., Galkowska H., Interewicz B., Olszewski W. L., Stelmach E., Luczak M., Machowski Z.
Medycyna Doświadczalna i Mikrobiologia
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2006
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tom 58
|
nr 4
269-274
Scharakteryzowano szczepy Staphylococcus aureus i Staphylococcus epidermidis, wyizolowane z ran i tkanek głębokich oraz z powierzchni skóry, pobranych w celu poszukiwania obecności genów dla czynników wirulencji. Określono genetyczne zróżnicowanie badanych szczepów uzyskane metodą reakcji PCR ze specyficznymi starterami.
11

Pokrewienstwo metycylinoopornych szczepow gronkowcow koagulazo-ujemnych

63%
Bogiel T., Mikucka A., Deptula A., Gospodarek E.
Medycyna Doświadczalna i Mikrobiologia
|
2009
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tom 61
|
nr 2
111-118
12

Fenotypowa charakterystyka gronkowcow koagulazo-ujemnych kolonizujacych dreny po zabiegach torakochirurgicznych u pacjentow z rakiem pluca

63%
Korona-Glowniak I., Los R., Malm A., Rybojad P., Furmanik F.
Medycyna Doświadczalna i Mikrobiologia
|
2003
|
tom 55
|
nr 2
109-115
Scharakteryzowano 33 szczepy gronkowców koagulazo-ujemnych izolowane z wydzieliny zalegającej w drenach chorych poddanych resekcji anatomicznej miąższu płuca z powodu raka. Najczęściej identyfikowano Staphylococcus epidermidis oraz S. warneri. Większość szczepów wykazywała zdolność do produkcji śluzu oraz właściwości hydrofobowe. Przeważały szczepy oporne na penicylinę i oksacylinę, ale wrażliwe na amoksycylinę z kwasem klawulanowym. Dwa szczepy S. haemolyticus wykazywały metycylinooporność.
13

Slime production by Staphylococcus aureus and Staphylococcus epidermidis strains isolated from patients with diabetic foot ulcers

63%
Podbielska A., Galkowska H., Stelmach E., Mlynarczyk G., Olszewski W. L.
Archivum Immunologiae et Therapiae Experimentalis
|
2010
|
tom 58
|
nr 4
321-324
14

Susceptibility of Propionibacterium acnes and Staphylococcus epidermidis to killing by MPO-halide system products. Implication for taurine bromamine as a new candidate for topical therapy in treating acne vulgaris

63%
Marcinkiewicz J., Biedron R., Bialecka A., Kasprowicz A., Mak M., Targosz M.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
|
tom 54
|
nr 1
15

The influence of long-lasting implantation on the function of phagocytes towards staphylococci

63%
Rozalska B., Burow A., Ljungh A., Rudnicka W.
Acta Microbiologica Polonica
|
1995
|
tom 44
|
nr 3-4
16

Lysostaphin as a potential therapeutic agent for staphylococcal biofilm eradication

63%
Walencka E., Sadowska B., Rozalska S., Hryniewicz W., Rozalska B.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 3
The aim was to study the activity of lysostaphin in monotherapy or in combination with oxacillin, towards biofilms built by clinical and reference S. aureus and S. epidermidis strains in the wells of microplate, in the chambers of a LabTekII chamber slide or on the polyethylene catheter. MICs of oxacillin and lysostaphin for planktonie bacteria were determined according to the standards of NCCLS. BIC (Biofilm Inhibitory Concentration) was estimated by the MTT assay. The integrity of biofilm treated with antimicrobials was also examined: by staining with FITC and laser scanning fluorescence confocal microscopy and visually by TTC reduction assay. Despite the fact that susceptibility of planktonie cultures of 25 staphylococcal strains to lysostaphin action was various, we have demonstrated the effectiveness of lysostaphin in the treatment of biofilm, built not only on the flat surface of the microplates but also on catheter's surface. The synergistic effect of subBIC lysostaphin+oxacillin was observed forMSSA and MRSA biofilms but not for 1474/01 hVISA strain. Also BICOXA for S. epidermidis RP12 and A4c strains, but not for 6756/99 MRSE biofilms was reduced when lysostaphin was simultaneously used.
17

Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci

63%
Nowak T., Balcerczak E., Mirowski M., Szewczyk E. M.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 4
The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CoNS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX₃₀) and oxacillin (OX₁), oxacillin agar screen test with 6 mg/l of oxacillin (MHOXA), latex test for PBP2a (LA) and detection of mecA via PCR. One hundred and one isolates were methicillin-resistant in at least one of methods used, but only 74 were mecA -positive. Of the 68 mecX-negative strains: two were positive by OX₁, the LA and MHOXA methods; three by the LA and MHOXA; and 22 only by OX₁, test. Most of these strains were from the novobiocin-resistant CoNS group. The results obtained for all tested strains using FOX₃₀ showed complete concordance with the presence of the mecA gene.
18

Different pro-inflammatory and immunogenic potentials of Propionibacterium acnes and Staphylococcus epidermidis: implications for chronic inflammatory acne

63%
Bialecka A., Mak M., Biedron R., Bobek M., Kasprowicz A., Marcinkiewicz J.
Archivum Immunologiae et Therapiae Experimentalis
|
2005
|
tom 53
|
nr 1
19

Cryptomeria japonica essential oil inhibits the growth of drug-resistant skin pathogens and LPS-induced nitric oxide and pro-inflammatory cytokine production

51%
Yoon W. J., Kim S.-S., Oh T.-H., Lee M. H., Hyun C.-G.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 1
61-68
In this study, the chemical composition of Cryptomeria japónica essential oil (CJE) was analyzed and its biological activities were tested. CJE was obtained by steam distillation from leaves collected from Jeju Island and analyzed by gas chromatography (GC)-flame ionization detection (FID) and GC-MS. Kaurene (17.20 %), elemol (10.88 %), y-eudesmol (9.41 %), and sabinene (8.86 %) were the major components in CJE. The antibacterial and anti-inflammatory activities of CJE against drug-susceptible and -resistant skin pathogens have been not reported previously. Thus, we determined the anti-bacterial activities of CJE using the disk diffusion method and minimum inhibitory concentration (MIC) values. CJE showed excellent antibacterial activities against Propionibacterium acnes and Staphylococcus epidermidis, which are acne-causing bacteria. The MIC of CJE against drug-susceptible and -resistant P. acens and S. epidermidis ranged from 0.16 to 10.0 μl/ml. In addition, the effects of CJE on nitric oxide (NO), prostaglandin E₂(PGE₂), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages were also examined. Pro-inflammatory cytokine and mediator tests indicated that CJE has excellent dose-dependent inhibitory activities. Therefore, based on these results, we propose that CJE is an attractive acne-mitigating candidate for skin health.
20

Effect of ciprofloxacin and N-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces

51%
El-Feky M. A., El-Rehewy M. S., Hassan M. A., Abolella H. A., Abd El-Baky R. M., Gad G. F.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 3
261-267
The aim of this study was to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. Two strains each of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klehseilla pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris, recently isolated from patients undergoing ureteral stent removal and shown to be capable of biofilm production, were used in this study. The inhibitory effects of ciprofloxacin, N-acetylcysteine and ciprofloxacin/N-acetylcysteine combination were determined by static adherence assay. Ciprofloxacin (MIC and 2 MIC) and N-acetylcysteine (2 and 4 mg/ml) inhibited biofilm production by ≥60% in all tested microorganisms. Disruption of pre-formed biofilms of all tested microorganisms was found to be ≥78% in the presence of ciprofloxacin (MIC and 2 MIC) and ≥62% in the presence of N-acetylcysteine (2 and 4 mg/ml), compared to controls. Ciprofloxacin/N-acetylcysteine showed the highest inhibitory effect on biofilm production (94-100%) and the highest disruptive effect on the pre-formed biofilms (86-100%) in comparison to controls. N-acetylcysteine was found to increase the therapeutic efficacy of ciprofloxacin by degrading the extracellular polysaccharide matrix of biofilms. These data are statistically significant. The inhibitory effects of ciprofloxacin and N-acetylcysteine on biofilm production were also verified by scanning electron microscope (SEM). In conclusion, Ciprofloxacin/N-acetylcysteine combinations have the highest inhibitory effect on biofilm production and the highest ability to eradicate pre-formed mature biofilms.
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