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The toxic effects of new quaternary ammonium chlorides on dicotyledonous plant Sinapis alba was evaluated. Investigated compounds differed either in hydrophobic chain length or hydrophilic part properties. The compounds used in the study are meant to be new wood preservatives because of their excellent fungicidal properties. It has been stated that the toxic effects of examined chlorides on Sinapsis alba depend on the compound structure. Alkyl(alkoxymethyl)dimethylammonium chlorides had the lowest phytotoxic properties. The presence of benzene subsituent in the compound molecule caused an increase in toxic effects of ammonium chlorides on the Sinapis alba roots.
The studies concerned the variability in nectar and pollen production in flowers of 75 double-low lines of white mustard (Sinapis alba L.) and a reference cultivar ‘Nakielska’. Also, rates of insect visitation to flowers in 19 lines were investigated. Significant genotypic differences were found in nectar amount, sugar concentration in nectar and total nectar sugar amount. The average nectar amount/10 flowers varied from 2.3 to 24.4 mg, with a mean 14.7 mg for ‘Nakielska’. Nectar concentration for most lines exceeded 20%. The majority (52%) of the lines tested produced more than 2 mg of sugars/10 flowers in nectar. The average amounts of pollen varied from 1.2 to 12.0 mg/10 flowers, with the mean 8.5 mg for ‘Nakielska’. No clear genotypic effect on the amount of pollen/10 flowers was demonstrated. The lines did not differed significantly in their attractiveness to flower visitors. The main foragers were honey bees (93% of all visiting insects). It is concluded that the double-low lines of S. alba are comparable to conventional cultivars in their potential for nectar flow but perform poorer as pollen producers. The studies concerned the variability in nectar and pollen production in flowers of 75 double-low lines of white mustard (Sinapis alba L.) and a reference cultivar ‘Nakielska’. Also, rates of insect visitation to flowers in 19 lines were investigated. Significant genotypic differences were found in nectar amount, sugar concentration in nectar and total nectar sugar amount. The average nectar amount/10 flowers varied from 2.3 to 24.4 mg, with a mean 14.7 mg for ‘Nakielska’. Nectar concentration for most lines exceeded 20%. The majority (52%) of the lines tested produced more than 2 mg of sugars/10 flowers in nectar. The average amounts of pollen varied from 1.2 to 12.0 mg/10 flowers, with the mean 8.5 mg for ‘Nakielska’. No clear genotypic effect on the amount of pollen/10 flowers was demonstrated. The lines did not differed significantly in their attractiveness to flower visitors. The main foragers were honey bees (93% of all visiting insects). It is concluded that the double-low lines of S. alba are comparable to conventional cultivars in their potential for nectar flow but perform poorer as pollen producers.
Stigmas isolated from flower buds of ‘Nakielska’ variety of Sinapis alba were used to develop a micropropagation method suitable for breeding of new cultivars. The origin of adventitious bud regeneration was studied on MS medium, under stimulation by bezylaminopurine (BAP) in combination with 2,4-D - naphtoxy acetic acid (2,4-D). Histological analysis showed the structure of Sinapis stigma (composed from four types of tissue: papillae, transmitting tissue, parenchyma and vascular bundles) and revealed that numerous meristematic centers developed from parenchyma cells in close vicinity of vascular bundles. Buds very quickly appeared on the surface of initial explants and later formed multiplantlets that were easily rooted in the soil.
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