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The main objective of this study was to determine the allelopathic impact of dominant species (Calamagrostis epigeios, Rubus idaeus and Chamaenerion angustifolium) of clear-cuttings of Scots pine (Pinus sylvestris L.) forests (Vaccinio-myrtillo Pinetum) on test species germination emphasizing the forest ecosystem establishment. Aqueous extracts of roots and shoots were produced at different growth stages and assayed on germination. Additionally, total concentration of phenols was evaluated photo spectrometrically. Extracts of shoots more strongly inhibited germination than those of roots of all investigated species. The strongest inhibitory effect on germination and the highest phenol contents were documented during the flowering stage rather than in spring and autumn. Accordingly, to mean germination data the declining phytotoxicity sequence of the species was determined: R. idaeus > C. epigeios > Ch. angustifolium. Hence this study implied in allelopathic activity of species a potential suppressive factor that could influence germination and forest regeneration, but field condition studies are necessary.
This paper reports the construction a map based on Amplified Fragment Length Polymorphic DNA (AFLP) in Scots pine (Pinus sylvestris L.). The main purpose of map construction was its application to quantitative traits loci (QTL) mapping for breeding traits economically important in Scots pine breeding program such as tree height and diameter at breast height, number of needles and their length, width, and area. Genomic DNA of needles and haploid megagamethophytes from seeds originating from a single tree were amplified with 25 AFLP primer-enzyme combinations with three or four selective nucleotides. Sixteen of them generated easily readable patterns and revealed a polymorphism. Each analyzed marker was tested for the expected 1 : 1 segregation ratio using χ2 – test and only 6 were significant with (α ≤ 0.05). The total map size equaled 291,7 cM and all markers were distributed within one linkage group. For all traits only one QTL associated with tree height (H) was detected.
Incidence of Armillaria root disease and the population structure of associated Armillaria spp. were studied in 5-17-year-old Scots pine plantations in west-central Poland. Two infection centres (1.14– 9.30 ha) in each of three forest districts (Siemianice, Zielonka and Złotów) were intensively sampled. Root collars were examined for mycelial fans, decayed wood, and rhizomorphs. Twenty two isolates of Armillaria ostoyae collected from epiphytic rhizomorphs from 20 living and two dead trees in the six infection centres were identified with somatic incompatibility group. Only one somatic incompatibility group for A. ostoyae was found. Twenty one isolates produced rhizomorphs on oak-wood discs submerged in a sand-forest soil substrate. Isolates from Siemianice formed the smallest rhizomorph networks and those from Złotów the most abundant. There were 16 different genets among 22 isolates of A. ostoyae distinguished by RAPD analysis. Genetic similarity among genets was 25.6–97.5%. The large diversity in A. ostoyae suggests that sexual reproduction may occur in nature more often than expected.
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Effect of soil bulk density on forest tree seedlings

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The paper presents the results of an analysis of the influence of soil bulk density in a forest nursery plot on the growth and quality parameters of Scots pine and European beech seedlings. Particular density variants were obtained using a tractor device exerting controlled pressure on the soil, while field examinations were performed on an area of ‘Kłaj’ forest nursery in Niepołomice Forest District. Three series of plots were prepared for each species, applying a unit pressure of the values of 50, 100, 150, 200, 250 kPa, corresponding to the dry bulk density in the range of 1.03-1.19 g cm-3, and control plots without the pressure. Seeds of the examined species were sown on the prepared plots, and after 6 months of growth the seedlings were subjected to biometric analysis determining differentiation in root neck diameter, length of the above-ground part and root system, as well as dry mass of particular parts of the plant. The quality of the seedlings was also determined using the method of Schmidt-Vogt. The results obtained show that the change in dry bulk densitysoil significantly affected most of the growth parameters of theexamined seedlings. Especially high negative correlations were obtained for the length and dry mass of the root system. A significant influence of dry bulk density variant on all growth parameters of Scots pine seedlings, and on some parameters of European beech was demonstrated. An increase in soil bulk density clearly caused also a deterioration of European beech seedlings quality.
This paper presents the results of studies on the influence of soil preparation methods and ways of utilization of logging residues on belowground biomass of 3-year-old Scots pine saplings, after artificial regeneration. Statistically significant correlations were detected between root biomass and soil preparation methods, while there was no significant impact of logging residues management. Furthermore, interaction of methods of soil preparation and various ways of logging residues management also did not affect the belowground biomass of the plants. The greatest biomass was detected on the area where the soil was prepared by ploughing furrows with the LPz-75 plough. On plots where the soil was prepared by scarifying with rotary tiller, the lowest values of belowground biomass were observed.
The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease (PWD). This nematode is considered to be an indigenous to North America and was introduced to Japan in the late 19th century. Subsequently, it has spread throughout Japan and in many other countries, China, Taiwan, and South Korea. In 1999, B. xylophilus was discovered in Portugal, and in 2008 in Spain. So far the studies have revealed that the pathogenicity of B. xylophilus varies between different isolates. The conducted study compared the pathogenicity of five isolates of B. xylophilus, originating from different parts of Japan, to 3-year-old Pinus sylvestris, and their ability to reproduce in the seedlings. The results revealed diverse virulence of B. xylophilus resulting in plant mortality. Three isolates S10, Ka4, and T4 caused 100% mortality of plants within three months while at the same time, the other two isolates, C14-5 and OKD-1 did not cause any disease symptoms on plants. After seven months, some dieback occurred on two seedlings, but similar symptoms were also found on the control plant. Moreover, a significant positive correlation was found between nematode virulence and the number of nematodes reproducing on pine seedlings.
There were formulated two general approaches to the problem under consideration indicating the experimental data sources. Mathematical symbols were introduced to denote each plant part and describe its function. There was constructed a discrete two – dimensional dynamical system that depicted Scots pine growth process followed by making suitable assumptions on its components. It has been found that this system is an iteration sequence of certain area transformation which served as the basis for the dynamical system development. Consequently, several important results like theorems, comments and charts were obtained. The obtained research results are related to variations of total dry weight Mn that grows exponentially, while linearly for n large enough. It was also demonstrated that the proportion of assimilatory to non assimilatory parts λ ̅ tends to the calculated stationary point λ1.
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