The study aimed at determining the effect of a multiple dose of lysozyme dimer (Lydium-KLP Nika Health Products) on primary humoral response in SRBC - immunized mice. The effects of a single (20 µg/kg) lysozyme dimer administered 2 h prior to antigen injection were compared with those of lysozyme dimer multiple doses (two-four) administered at one week intervals. The results of the study show that lysozyme dimer, irrespective of the number and range of the doses (2, 20 or 200 µg/kg) enhances humoral response to the antigen. The adjuvant action of lysozyme dimer is expressed in the increasing number of splenocytes producing hemolytic antibodies (PFC) and the total and 2-mercaptoethanol resistant level of anti-SRBC hemagglutinins. The strongest activation of the immune response was observed after a single administration and multiple injections at doses of 2 or 20 µg/kg. Three doses of lysozyme dimer (200 µg/kg each) injected at 7-day intervals decreased the number of PFC induced by this drug administered in a single dose of 20 µg/kg 2 h prior to immunization, but maintaining the potentializing impact on anti-SRBC antibody production. The results obtained in the study indicate that multiple doses of lysozyme dimer administered in doses recommended in veterinary practice (2-20 µg/kg), at 7-day intervals do not results in immunological tolerance.
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