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An intracellular aminopeptidase N synthesized by Pseudomonas putida Lup was purified and characterized. The approx. 150-fold purified enzyme showed highest activity against A-β-naphthylamide at pH 7.5 and at temperature 40°C and was 100% thermostable for 240 min at 40°C. P. putida lup aminopeptidase N is a monomer with molecular mass approx. 99 kDa determined by SDS-PAGE and gel permeation chromatography. The enzyme has broad substrate specificity, but is the most active against protein substrates with N-terminal alanine and arginine. The activity of P. putida Lup aminopeptidase N is strongly inhibited in the presence of specific metallopeptidase inhibitors and is partly recovered in the presence of Zn²⁺ and Co²⁺ ions. Co²⁺, Mg²⁺ and Ca²⁺ ions increased the activity of the enzyme. Moreover, the enzyme was inhibited by inhibitors of cysteine enzymes. Analysis of fragments of the amino acid sequence of the purified enzyme demonstrated high similarity to PepN of Pseudomonas putida GB-1.
Fluorescent pseudomonads are among the most influencing plant growth-promoting rhizobacteria in plants rhizosphere. In this research work the plant growth-promoting activities of 40 different strains of Pseudomonas fluorescens and Pseudomonas putida, previously isolated from the rhizosphere of wheat (Triticum aestivum L.) and canola (Brassica napus L.) and maintained in the microbial collection of Soil and Water Research Institute, Tehran, Iran, were evaluated. The ability of bacteria to produce auxin and siderophores and utilizing P sources with little solubility was determined. Four strains of Wp1 (P. putida), Cfp10 (Pseudomonas sp.), Wp150 (P. putida), and Wp159 (P. putida) were able to grow in the DF medium with ACC. Thirty percent of bacterial isolates from canola rhizosphere and 33% of bacterial isolates from wheat rhizosphere were able to produce HCN. The results indicate that most of the bacteria, tested in the experiment, have plant growth-promoting activities. This is the first time that such PGPR species are isolated from the Iranian soils. With respect to their great biological capacities they can be used for wheat and canola inoculation in different parts of the world, which is of very important agricultural implications.
In this study the biodegradation of oil by hydrocarbon degrading Pseudomonas putida in the presence of a biosurfactantproducing bacterium was investigated. The co-culture of test organisms exhibited improved degradation capacities, in a reproducible fashion, in aqueous and soil matrix in comparison to the individual bacterium culture. Results indicate that the in situ biosurfactant production not only resulted in increased emulsification of the oil but also change the adhesion of the hydrocarbon to cell surface of other bacterium. The understanding of interactions beetwen microbes may provide opportunities to further enhancement of contaminants biodegradation by making a suitable blend for bioaugmentation.
A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.
Badaniami objęto 34 szczepy P. putida, z których 27 izolowano z gleby zanieczyszczonej pochodnymi ropy naftowej, 3 z biopreparatów stosowanych w procesach biodegradacji zanieczyszczeń oraz 4 z ran i owrzodzeń. Podjęto próbę wewnątrzgatunkowego różnicowania szczepów P. putida wykorzystując ich aktywność biochemiczną, wzory lekowrażliwości i określając zymotypy. Stwierdzono przydatność tych metod w diagnostyce mikrobiologicznej pałeczek P. putida.
The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.
This investigation was conducted to evaluate the effects of four strains of plant growth promoting rhizobacteria (Agrobacterium rubi A-18, Bacillus subtilis OSU- 142, Burkholderia gladioli OSU-7 and Pseudomonas putida BA-8) on growth and leaf nutrient content of 'Starking Delicious', 'Granny Smith', 'Starkrimson Delicious', 'Starkspur Golden Delicious' and 'Golden Delicious' apple cultivars grafted on semi-dwarf rootstock MM-106. The applications of bacterial strains increased the leaf number and area as well as number of annual shoots and their diameter, although OSU-7 application suppressed annual shoot length. BA-8 application resulted in the highest annual shoot number (52.4) and OSU-142 in the largest leaf area (16.12 cm2). The applications of A-18 bacteria decreased the concentration of N, K and Cu and increased the concentration of P and Zn in the leaves. OSU-142 application resulted in the highest Mg (0.13%) and Fe (32.7 ppm) contents and OSU-7 in the highest Mn content (40.3 ppm). None of the applications affected the concentration of Na and Ca in the leaves.
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