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In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

100%

Kasprzyk-Pawelec A., Pietrusiewicz J., Szczuka E.

Acta Scientiarum Polonorum. Hortorum Cultus

2015

tom 14

nr 4

In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Kasprzyk-Pawelec A.

1 Pietrusiewicz J.

1 Szczuka E.

1 2015

Wyniki wyszukiwania

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’