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Soil respiration is a very important factor influencing carbon deposition in peat and reflecting the intensity of soil organic matter decomposition, root respiration, and the ease of transporting gases to the surface. Carbon dioxide release from three different peat soil profiles (0-80 cm) of the Polesie Lubelskie Region (Eastern Poland) was analyzed under laboratory conditions. Peat samples were incubated at 5, 10, and 20°C in aerobic and anaerobic environments, and their CO2-evolution was analyzed up to 14 days. The respiration activity was found to be in the range of 0.013-0.497 g CO2 kg-1 DW d-1. The respiratory quotient was estimated to be in the range of 0.51-1.51, and the difference in respiration rates over 10°C ranged between 4.15 and 8.72 in aerobic and from 1.15 to 6.53 in anaerobic conditions. A strong influence of temperature, depth, the degree of peat decomposition, pH, and nitrate content on respiration activity was found. Lack of oxygen at low temperature caused higher respiration activity than under aerobic conditions. These results should be taken into account when the management of Polish peatlands is considered in the context of climate and carbon storage, and physicochemical properties of soil in relation to soil respiration activity are considered.
The potential of methanotrophic activity (MTA) has been investigated under labo-ratory conditions in three types of peatland profiles: high (H), transition (T) and low (L) originating from Polesie Lubelskie Region. Selected peat samples differed in respect of pH, TOC, von Post index and moisture. The experiment was conducted at natural moisture (198-719 %w/w) with dif-ferent ranges of both, temperature (5, 10 and 20°C) and CH4 enrichment (1 and 5%v/v). The highest MTA (19.69-155.79 mg CH4kg D.W.-1 d-1) was observed at 20°C. Regardless of temperature, MTA was lower (1.38-51.16 mg CH4 kg D.W.-1 d-1) when peat samples were incubated in atmosphere enriched in 1% than in 5% CH4 v/v (4.75-191.26 mg CH4kg D.W.-1 d-1). Strong influence of tem-perature and sampling sites on MTA was also noted. Total DNA was isolated from the most active (20°C, 5% CH4 v/v) peat samples from each site and the PCR (polimerase chain reaction) amplify-ing of genes pmoA (primers A189f/mb661r) and sequence 16S rRNA (primers Type If /Type Ir and Type IIf/Type IIr) specific for methanotrophic bacteria were carried out. Positive results of PCR with primers of pmoA gene after sequencing confirmed that methanotrophs from L point belong to family Methylococcaceae, while 16S rRNA gene sequences from microorganisms inhabiting H peat demonstrated the highest similarity to genus Methylocystis and Methylosinus.
The bottom fauna inhabiting two types of ecotones: 1) the transition zone between land and lake water, and 2) the border of a macrophyte bed and open water, situated in different types of littoral, distinguished mainly according to the structure and distribution of macrophytes was studied. The investigations were carried out in five harmonious lakes (from mesotrophic to highly eutrophic) and in one disharmonious (humoeutrophic) in July and September of 1996 and 1997. Both the species composition of zoobenthos and the relative abundance of the most numerous taxa (Tubificidae and larvae of Chironomidae) in ecotones did not seem to depend much on the type of littoral but rather upon the nature of the bottom sediments. The influence of lake fertility in harmonious lakes was clearly expressed mostly in the land-water ecotones where it was demonstrated by the drop of chironomids and increase in tubificids and predators in total faunal abundance with a rise of lake. trophism. The clear pattern in vegetation bed-open water ecotones concerned solely predators which contribution to the total faunal abundance tended to increase with a rise of lake trophic status. The ecotones of the disharmonious lake did not show much differences in comparison with the harmonious lakes except extremely low faunal density in vegetation-open water ecotone, being totally predominated by chironomids. In both harmonious lakes and in disharmonious one the highest faunistic similarities were found within the same types of ecotones, while the two types differed greatly.
Salix myrtilloides L. is an endangered species whose western limit of range runs through Poland. The main aim of the study was to increase the knowledge on the ecology and biology of S. myrtilloides populations in the Polesie Lubelskie region (Eastern Poland) in order to create an effective protection program. An 80% decrease in its population was found in this area. Our study was conducted to identify the mechanisms responsible for the process of withdrawal of this species from its natural stands by determining whether the processes of generative reproduction (pollen viability and germinability, seed germination ability and dynamics) in the populations occur properly and by characterizing within- and among-population genetic diversity of S. myrtilloides, using ISSR (Inter-Simple Sequence Repeat) primers. The results confirmed that S. myrtilloides pollen viability was high (84.17±8.67), and so were the seed germination ability (76% of the total number of sown seeds/24 h) and dynamics. The within-population genetic diversity was quite high for this species and the inter-population genetic variation was of medium value (ΦPT=0.148). The condition of two populations, their genetic diversity and sex ratio as well as the correctness of the investigated reproductive cycle stages altogether give prospects for their survival. It seems to be very important to start conservation involving the reintroduction and reconstruction of S. myrtilloides populations in the Polesie Lubelskie region. The proposed method for restoration of S. myrtilloides resources would be possible if ex situ cultivation and in vitro methods were used.
The main aim of the paper was to study the effect of coagulation of copper ions on dissolved form of humic acids. Research material was isolated from four peaty muck soils (Terric Histosols). The soils were described by means of the Springer humification number, secondary transformation index, ash content and pH in H2O and in KCl. For the studied humic acids, surface charge was determined, E4/E6 which described the degree of concentration of the chain of aromatic carbons of the humic acids and could be used as their humification number, and the elemental composition and atomic ratios were determined as well. Systems composed of humic acids and increasing concentrations of copper ions were investigated at pH 5 and in the copper concentration range of 0-40 mg dm-3, at constant concentration of humic acids. Coagulation of solutions was studied by measurement of changes of organic carbon and copper ions concentration in liquid phase at pH 5 as well as by observation of absorbance changes at wavelength 465 nm in series of prepared solutions. The results showed that coagulation of experimental systems consisted mainly in humic acid coagulation. In a certain range of concentration of copper added to solutions, metal also partly precipitated and went to the solid phase together with humic carbon, which indicated coagulation of complexed compound of humic acids with copper. At high copper concentration, ionic strength had a dominant influence on coagulation of humic acids. Copper ions stayed all in liquid phase, whereas almost total organic carbon passed to precipitate, which resulted from interactions with electrolyte ions.
The studies were carried out in the littoral zone of six lakes differing in trophic and biotic diversity. These were: L. Piaseczno - the mesotrophic lake with the well developed psammolittoral, L. Uściwierz - the slightly eutrophic lake with the psammolittoral and phytolittoral, the hypertrophic lake L. Głȩbokie Uścimowskie with the psammolittoral, L. Bikcze and L. Łukie - both the eutrophic lakes with phytolittoral, and the dystrophic L. Moszne - with peatbog phytolittoral. During the spring and the summer-autumn seasons in 1996-1997 the physical and chemical composition (temperature, electrolytic conductivity, O₂, pH, total water hardness, nitrogen compounds and phosphorus compounds) of water were investigated in the littoral zone in these lakes.
The ecotonal zone was created by psammolittoral, emerged macrophytes in eulittotal and littoral, and submerged vegetation in littoral. Immigration of typical plankton species of Rotifera to the psammon communities occurred. The highest density of rotifers was found in psammolittoral zone, lower in littoral and the lowest in eulittoral zone. The highest biodiversity was found in eulittoral and littoral zones of lakes with well-developed pond type phytolittoral.
The chemical composition and biotic structure were studied in ecotonal zones of six trophically differentiated lakes in the region of Polesie Lubelskie (Eastern Poland). High qualitative and quantitative differentiation of zoocenoses (zooplankton and zoobenthos) usually occurred in each ecotonal zone of every lake studied. The highest diversity occurred among microinvertebrates (zoopsammon and zooplankton).
Intense changes in the land use of lake catchment basins in the Polesie Lubelskie region (E. Poland), observed over the last decades, have a strong, significant effect on the functioning of lakes and wetlands in the landscape. One of the methods of investigating these changes is analysis of the network of land use boundaries. The studies included 11 basins of lakes located in 3 neighboring river valleys. Basing on the analysis of air photographs from the years 1952 through 1992, 55 types of land use boundaries (transitional zones) had been identified in that region. The changes, which had taken place during this period, related to the features like: character of the transitional zones, density of their network and size of the patches were also examined. Most stable and most transformed areas were pointed out. Suggestions concerning directions and methods of environmental protection in that region were formulated.
Ecotone zones are very interesting objects for the investigations of the ecological succession of plant communities. The main aim of the present study was to analyse the rate and direction of changes in ecotone vegetation caused by changes in a level of groundwater table. In the studies carried out during years 1993-1998 a generally accepted method of a belt transect was applied. Transect 5m wide ran across all aquatic, litoral and land phytocenoses. Most visible changes took place in the aquatic and littoral phytocenoses. Some of them disappeared and then reconstructed themselves within a new range, in others the species composition was changed.
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