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1

Evaluation of Wondfo Rapid Diagnostic Kit (Pf-HRP2/PAN-pLDH) for diagnosis of malaria by using nano-gold Immunochromatographic assay

100%
Wu J., Peng Y., Liu X., Li W., Tang S.
Acta Parasitologica
|
2014
|
tom 59
|
nr 2
Prompt and accurate diagnosis is necessary to start adequate treatment for different affecting species including P. falciparum and P. vivax. Here we described the Wondfo Rapid diagnostic Kit (Pf-HRP2/PAN-pLDH) for the detection of P. falciparum and pan-plasmodium in patient specimen by using a nano-gold immunochromatographic assay. Our rapid assay adapted nano-gold labeling techniques and the monoclonal antibodies (mAbs) against both histidine rich protein-2 (Pf HRP-2) of P. falciparum and pan plasmodium-specific pLDH (pan pLDH). The established two-antibody sandwich immunochromatographic assay could detect P. falciparum and pan-plasmodium. The sensitivity and specificity of Wondfo rapid diagnostic kit were determined by comparing with the “gold standard” of microscopic examination of blood smears. In this study1023 blood samples were collected from outpatient clinics in China and Burma, and detected by both Wondfo kit and microscopic examination. The detection sensitivity and specificity of Wondfo rapid diagnostic kit were 96.46% and 99.67% for P. falciparum (HRP2), 95.03% and 99.24% for pLDH, 96.83% and 99.74% for non-falciparum species, 96.70% and 99.74% for P. vivax, respectively. These results indicate that Wondfo rapid diagnostic assay may be useful for detecting P. falciparum and non-P. falciparum (especially P.v.) in patient specimen.
2
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Zimnica wspolistniejaca z zakazeniem HIV

84%
Siwak E., Kowalczuk-Kot A., Pogorzelska J.
Wiadomości Parazytologiczne
|
2006
|
tom 52
|
nr 1
9-11
The coexistence of malaria and HIV infection beyond inhabitants of sub-Saharan Africa, South America and South-East Asia arises a question whether there is an interaction between these two infections. This problem is extremely important in relation to pregnant women because of possibility of mother to child transmission. The available options are reviewed in the paper.
3

Prevalence and distribution of human Plasmodium infection in Federally Administrative Tribal Areas of Pakistan

84%
Hussain I., Qureshi N. A., Afzal M., Shaheen N., Ali A., Ashraf A.
Acta Parasitologica
|
2016
|
tom 61
|
nr 3
4
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Autoimmunologiczna trombocytopenia w nawracajacej polietiologicznej malarii [Plasmodium falciparum, Plasmodium vivax]

84%
Panasiuk A.
Wiadomości Parazytologiczne
|
2001
|
tom 47
|
nr 1
85-89
Thrombocytopenia frequently appear in severe malaria. The reasons of low blood platelets count are ditferent and its results of hypersplenism, subclinical course of intravascular coagulation (DIC). Thrombocytopenia from "consumption" is consequence of sequestration of blood platelets in blood vessels of lungs and cerebral. We examination 29 years old men, who was as forest worker in islands on Indonesia. He was treated with recurrent, poliethiological malaria (Plasmodium falciparum, Plasmodium vivax) and severe thrombocytopenia (17.0 GIL) without hepatosplenomegalia. Antiplatelet antibody was examined in blood serum by ELISA methods (GTI - PAKPLUS®). In blood serum was detected IgG antibody against glicoprotein receptors on surface of blood platelets GPIIb/IIIa, GPIV, GPIb/IX, GPV, GPIa/IIa. Chronic infections of Plasmodium may conduct to autoimmune destruction of blood platelets.
5

Preliminary analysis of Plasmodium vivax genotypes isolated in Southeastern Turkey

84%
Doskaya A. D., Doskaya M., Caner A., Gul K., Nergiz S., Can H., Guruz Y.
Acta Parasitologica
|
2015
|
tom 60
|
nr 2
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Criteria of clinical evaluation of malaria patients after their return from Tropical countries

84%
Kociecka W., Rehlis N., Kurczewska M., Mrozewicz B.
Wiadomości Parazytologiczne
|
1998
|
tom 44
|
nr 3
7

Clinico-pathological studies of Plasmodium falciparum and Plasmodium vivax – malaria in India and Saudi Arabia

67%
Khan W., Zakai H. A., Asma U.
Acta Parasitologica
|
2014
|
tom 59
|
nr 2
Malaria is one of the most devastating diseases of tropical countries with clinical manifestations such as anaemia, splenomegaly, thrombocytopenia, hepatomegaly and acute renal failures. In this study, cases of thrombocytopenia and haemoglobinemia were more prominent in subjects infected with Plasmodium falciparum (Welch, 1897) than those with Plasmodium vivax (Grassi et Feletti, 1890). However, anaemia, jaundice, convulsions and acute renal failure were significantly high (3–4 times) in subjects infected with P. falciparum than those infected with P. vivax. The incidence of splenomegaly and neurological sequelae were 2 and 6 times higher in P. falciparum infections compared to the infections of P. vivax. Both in P. vivax and P. falciparum malaria, the cases of splenomegaly, jaundice and neurological sequelae were almost double in children (<10 years) compared to older patients. The liver enzymes were generally in normal range in cases of low and mild infections. However, the AST, ALT, ALP activities and serum bilirubin, creatinine, and the urea content were increased in P. falciparum and P. vivax malaria patients having high parasitaemia, confirming liver dysfunction and renal failures in few cases of severe malaria both in India and Saudi Arabia.
8

Vivax malaria presenting with cerebral malaria and convulsions

67%
Anvikar A. R., Singh D. K., Singh R., Dash A. P., Valecha N.
Acta Parasitologica
|
2010
|
tom 55
|
nr 1
96-98
A patient was admitted with fever, vomiting, restlessness and convulsions. He was febrile and unconscious. Laboratory tests showed a low platelet count and ruled out enteric fever and dengue. His peripheral blood smear was positive for Plasmodium vivax. The presence of P. vivax monoinfection was confirmed by polymerase chain reaction and DNA sequencing. The report highlights the importance of considering the possibility of complications even in P. vivax malaria and formulation of strategies accordingly.
9

Genetic diversity in human malaria parasites of Khyber Agency Pakistan

67%
Khatoon L., Jan M. I., Khan I. U., Ullah F., Malik S. A.
Acta Parasitologica
|
2013
|
tom 58
|
nr 4
Malaria is wide spread in poor world and its burden has been assessed by the enumeration of malarial parasites in blood of patients. This study was designed to find a relationship between social structure, and spread of malaria in Khyber agency. The average parasite density was 2050 parasite/μl in Khyber Agency. Due to economic and social setup most of the people have habit of sleeping in open air thus playing role in high malaria prevalence and Plasmodium vivax remains the prevalent species. Genetic study performed on 110 Blood samples showed less genetic diversity for both Plasmodium vivax and Plasmodium falciparum. Eight alleles were distinguished both for Pvmsp 3α and Pvmsp 3β in total of 20 and 39 amplified samples of P. vivax respectively. Out of 17 samples amplified for P. falciparum 11 showed genotype K1 and 10 for MAD at Pfmsp-1 while 14 alleles were identified for 3D7/1C and two for FC27 of corresponding families of Pfmsp-2 gene. This shows that Plasmodium parasites are not genetically diverse in Khyber agency.
10

Field evaluation of OptiMAL48 rapid malaria diagnostic test in India

67%
Valecha N., Singh N., Yadav R. S., Dev V., Aggarwal A., Subbarao S. K.
Acta Parasitologica
|
2003
|
tom 48
|
nr 3
A rapid malaria diagnostic test, OptiMAL®, was evaluated in a field based study in different epidemiological zones of India. The test can differentiate P. falciparum and non-P. falciparum malaria. The OptiMAL test is based on a dipstick principle for detection of parasite lactate dehydrogenase (pLDH). Since it has been shown that HRP-2 antigen can be detected even after parasitological cure, pLDH detection is more suited for follow up of patients. The sensitivity and specificity of the test compared to microscopy was evaluated in 699 febrile patients from all the study sites. The sensitivity and specificity obtained with the OptiMAL test was 87.9% (79-100%) and 98.9% (95.4-100%) respectively, for diagnosis of falciparum malaria while sensitivity and specificity of OptiMAL for diagnosis of non-falciparum malaria (P. vivax) were 93% (89.2-100%) and 96.8% (94.3-100%), respectively. However, failure to detect high parasitaemia in one patient with P. falciparum malaria is of particular concern. Although the rapid tests have a definite role in diagnosis of malaria especially where expertise for microscopy is not available, the cost of the test kits remains a major obstacle to their wide spread use in developing countries.
11
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Selected parasitic and infectious diseases in persons returning from The Tropics

67%
Jeske J., Bergiel A., Kamerys J.
Wiadomości Parazytologiczne
|
1998
|
tom 44
|
nr 3
12

Malaria wciaz grozna

59%
Hempelmann E., Tesarowicz I., Oleksyn B.
Wszechświat
|
2008
|
tom 109
|
nr 07-09
180-189
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