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Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos

100%

Wawrzyniak M., Hazubska-Przybyl T., Obarska A., Bojarczuk K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

2

Micropropagation of Picea abies (L.) Karst and P. omorika (Pancic) Purk. by somatic embryogenesis

100%

Hazubska T., Bojarczuk K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

3

Somatic embryogenesis of selected spruce species [Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana]

100%

Hazubska-Przybyl T., Bojarczuk K.

Acta Societatis Botanicorum Poloniae

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2008

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tom 77

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nr 3

189-199

Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g-1"1). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.

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First notice of Phytophthora tip blight on Picea omorika and Thuja occidentalis in Poland

100%

Orlikowski L. B., Szkuta G.

Phytopathologia Polonica

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2003

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tom 28

5

Swierki na naszej dzialce

80%

Bielenin M.

Działkowiec

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1999

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nr 10

12-14

6

Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies

80%

Hazubska T., Szczygiel K.

Dendrobiology

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2003

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tom 50

The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.

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Morphological and anatomical factors responsible for varied susceptibility of some species of spruce to infestation by spruce spider mite [Oligonychus ununguis Jacobi]

80%

Puchalska E., Tykarska T., Czajkowska B.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 6

Differences in susceptibility to infestation by spruce spider mite of 3 investigated spruce species (Picea glauca ‘Conica’, P. pungens and P. omorika) may be due to features of anatomical and morphological structure of needles. In P. omorika, showing some resistance to the spruce spider mite, we noted lamellar structure of epidermal cell walls and extensive supporting tissues (hypodermis and sclerenchyma fibres) at the whole circumference of the needle.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Acta Physiologiae Plantarum

1 Acta Societatis Botanicorum Poloniae

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Dendrobiology

1 Działkowiec

1 Phytopathologia Polonica

3 Bojarczuk K.

2 Hazubska T.

2 Hazubska-Przybyl T.

1 Bielenin M.

1 Czajkowska B.

1 Obarska A.

1 Orlikowski L. B.

1 Puchalska E.

1 Szczygiel K.

1 Szkuta G.

1 Tykarska T.

1 Wawrzyniak M.

1 2015

1 2008

1 2006

1 2005

2 2003

1 1999

Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos

Micropropagation of Picea abies (L.) Karst and P. omorika (Pancic) Purk. by somatic embryogenesis

Somatic embryogenesis of selected spruce species [Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana]

First notice of Phytophthora tip blight on Picea omorika and Thuja occidentalis in Poland

Swierki na naszej dzialce

Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies

Morphological and anatomical factors responsible for varied susceptibility of some species of spruce to infestation by spruce spider mite [Oligonychus ununguis Jacobi]