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1

A simple, direct plating method, alternative to dilution plating, for estimation of the abundance of Penicillium verrucosum on incubated cereal grain

100%
Czaban J., Wroblewska B.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 3
Because dilution plating is more labor intensive than direct plating, we would like to propose the use of a direct plating technique for estimation of Penicillium verrucosum abundance in cereal grain in incubation studies, with use of very selective and indicative for the fungus agar DYSG medium. The proposed method is based on the measurement of the diameter of P. verrucosum colonies grown around cereal kernels placed on DYSG medium. In three different experiments wheat grain contained a great range of P. verrucosum CFU number (from <25 to 77 x 10⁶ per 1 g). When P. verrucosum CFU number was at least as high as 10² per 1 g of the grain, 100% of the wheat kernels, placed on the surface of DYSG medium, were surrounded by colonies of P. verrucosum. The diameter (x, mm) of P. verrucosum colonies surrounding wheat kernels on DYSG medium was correlated with the fungal CFU number (y) on the wheat grain. The relationship is described by the exponential regression equation (y = 0.1258 e 0.9309x, R²=0.96). The relationship became linear (y = 0.404 x-0.901, R² = 0.96) after transformation of P. verrucosum CFU numbers to logarithms to base 10.
2

Growth of Penicillium verrucosum and production of ochratoxin A on nonsterilized wheat grain incubated at different temperatures and water content

84%
Czaban J., Wroblewska B., Stochmal A., Janda B.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 4
The results of two experiments with wheat grain inoculated with Penicillium verrucosum are reported. In Experiment I, wheat grain, containing 10, 20 and 30% water, was incubated for 2 weeks at 10, 15, 21 and 28°C. In Experiment II, wheat grain, containing 14, 16, 18, 20 and 22% water, was incubated for 2 weeks at 10, 15, and 20°C. At initial moisture content (IMC) of the wheat grain up to 16% neither P. verrucosum growth nor ochratoxin A (OTA) formation were observed. In the range of IMC from 18% to 22% both the fungal growth and OTA synthesis were distinct, and the parameters were higher at higher temperature in the range 10-21°C. A temperature of 28°C was probably too high for proper metabolism of the fungus, including OTA formation. OTA formation was distinctly related to P. verrucosum abundance in the temperature range 10-21°C, expressed both as the counts of fungal colony forming units (CFU) on agar DYSG medium and diameters of the fungal colonies growing around the wheat kernels placed on the surface of DYSG medium. OTA formation and abundance of P. verrucosum were negatively correlated with the percentage of wheat kernels, placed on DYSG medium, with growing colonies of fungi different from P. verrucosum. CFU counts of P. verrucosum on the wheat grain were significantly related to the diameter of the fungal colonies growing around the wheat kernels placed on DYSG medium. The relationship is described by an exponential regression equation.
3

Ocena ryzyka przy pracach budowlanych. Część 11: Czynniki biologiczne - prace murarsko-tynkarskie

67%
Bozejewicz E.
Atest
|
2014
|
nr 06
4

Tulipany

67%
Skrzypczak C.
Owoce, Warzywa, Kwiaty
|
2005
|
nr 04
5
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Levels of ochratoxin A in IgG against conidia of Penicillium verrucosum in blood samples from healthy farm workers

67%
Skaug M. A.
Annals of Agricultural and Environmental Medicine
|
2003
|
tom 10
|
nr 1
Ochratoxin A (OTA) is a mycotoxin frequently found in human blood and milk samples in the colder climatic zones. In addition to dietary intake, exposure may occur by inhalation of toxin containing fungal conidia. The purpose of this work was to investigate the level of OTA in blood samples from farm workers and non-farm working controls, and to examine if serum levels of OTA were related to inhalatory exposure to conidia of Penicillium verrucosum, the main OTA producer in temperate climates. Blood samples from 210 participants were analysed for the presence of OTA and IgG antibodies against P. verrucosum conidia. The concentration of OTA was determined by HPLC (DL 10 ng/l), and the IgG level was determined by ELISA. All serum samples contained OTA (mean 397 ng/l, range 21-5534 ng/l). The OTA level in serum was unrelated to farm working, gender, age, and IgG level. The mean IgG level was significantly higher among farm workers than controls. Farm working, or increased inhalatory exposure to P. verrucosum, was not related to higher OTA serum levels. Inhalatory exposure to OTA from farm working seems to be of minor importance compared to dietary intake.
6

Preferencje roztoczy w wyborze ziarniakow pszenicy porazonych przez rozne gatunki grzybow

60%
Szlendak E., Wakulinski W., Boczek J.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 1
141-145
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