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Cell suspension of Papaver somniferum L. (opium poppy) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest saccharase activity was at pH 4.6 and 50°C. The hydrolysis of the substrate was linear for 5 h reaching 69% of conversion, A very good storage stability was achieved when using dry catalyst, or a solution of 0.15 M NaCl with the addition of chloramphenicol, (l-methyldodecyl)-dimethylamin-4-oxide (ATDNO), Chlortetracycline hydrochloride (CLCTC) or by freezing the immobilized cells in 0.15 M NaCl. The cells characterized by high enzyme activity and stability in long-term storage showed convenient technological and physicomechanical properties.
The study was carried out in the years 2013 and 2014 at the Łagiewniki farm to determine the effect of some herbicides on the yield and development of two opium poppy cultivars: ‘Lazur’ – with high morphine content, and ‘Borowski Biały’ – with low morphine content. The development and yield of the poppy was a derivative of environmental and agronomic conditions. The mixture of tembotrione and fluroxypyr applied post-emergence at a rate of 88 and 75 g a.i. · ha−1, respectively, effectively controlled most weeds which are harmful for the poppy and did not phytotoxically affect opium poppy plants. The level of yield also depended on the cultivar’s ability to grow under unfavourable weather conditions. A lower yield was noted for the cultivar with a lower content of morphine – ‘Borowski Biały’.
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In the course of a study of fungal biodiversity of opium poppy (Papaver somniferum) plants collected in production area of Slovakia, an ascomycete belonging to the genus Subramaniula was isolated. The fungus identified as Subramaniula thielavioides has been reported for the first time from Slovakia. This record also represents the first European locality. Brief morphological description of the fungus based on an isolate from flower petals of opium poppy is provided.
The aim of the presented studies was to determine the effect of chemical weed control on the development and yield of native poppy cultivars. The results of presented experiment showed that the native poppy cultivars are more susceptible to herbicides than ‘Opal’ and ‘Lazur’ cultivars. The yield of ‘Michałko’ cultivar in all treatments of chemical protection was very low. It was proved that the suggestion of a necessary protection of ‘Mieszko’ cultivar against weeds with lower doses of herbicides than the doses applied in the cultures of cultivars with a high content of morphine is justified. ‘Mieszko’ cultivar gave the highest yield on plots protected after sowing with Lentipur Flo™ 500 SC (a.s. chlorotoluron) in the dose of 1.2 l/ha and in the growth stage of 4 leaves, when the treatment was carried out with Callisto™ 100 SC (a.s. mesotrione) in the dose of 0.4 l/ha. Positive results of weed control after the application of these herbicides indicate the possibility to initiate studies aiming at authorization to use these herbicides in growing of the native cultivars of poppy (minor crop).
Effects of two doses of nitrogen (0.6 and 0.9 g N·pot⁻¹) in combination with foliar application of boron (10 mg B·pot⁻¹) and two levels of P and K were studied in a pot experiment with poppy plants (Papaver somniferum L.). After the application of boron, a marked increase of its concentration in plants (112-126 mg·kg⁻¹) was observed. As compared to variants without boron, higher B concentrations were found in variants with the supply of this element within the whole course of growing season. Concentration of nitrogen decreased boron content during growing season due to a dilution effect. However, there were no grater differences among individual variants. In combination with increased levels of N, P, K, the application of B on leaves increased the seed yield by 6.4% and decreased production of straw by 6.7% as compared to controls without supply of this element. This fact was manifested also in the narrowest straw/seeds ratio. The volume of one capsule ranged in individual variants from 13.1 to 16.6 ml per plant but without any direct dependence on seed yield. The application of boron was manifested only in combination with increased levels of N, P, K. Concentrations of morphine in straw (empty heads + 15 cm of stem) increased with the increasing supply of nitrogen from 0.40 to 0.49%. The effect of boron supply was not marked too much.
Culture of Papaver somniferum in vitro was used for a characterisation of cell surface structures and mode of cell adhesion and cell separation during cell differentiation and plant regeneration in somatic embryogenesis and shoot organogenesis. In early stages of somatic embryogenesis, cell type-specific and developmentally regulated change of cell morphogenesis was demonstrated. Cell wall of separated embryonic cells were self-covered with external tubular network, whereas morphogenetic co-ordination of adhered cells of somatic proembryos was supported by fine and fibrillar external cell wall continuum of peripheral cells, interconnecting also local sites of cell separation. Such type of cell contacts disappeared during histogenesis, when the protodermis formation took place. Tight cell adhesion of activated cells with polar cell wall thickening, and production of extent mucilage on the periphery were the crucial aspects of meristemoids. Fine amorphous layer covered developing shoot primordia, but we have not observed such comparable external fibrillar network. On the contrary intercellular separation of differentiated cells in regenerated organs, and accepting distinct developmental system of somatic embryogenesis and shoot organogenesis, cell adhesion in early stages and ultrastructural changes associated with tissue disorganisation, and the subsequent reorganisation into either embryos or shoots appear to be regulatory morphogenetical events of plant regeneration in vitro.
Celem prowadzonych badań było znalezienie optymalnych metod indukowania mutagenezy dla uzyskania nowej zmienności głównych alkaloidów maku: morfiny, tebainy i kodeiny. Materiał do badań stanowiły genotypy linii hodowlanych pochodzące z rekombinantów różnych form maku wysokomorfinowego z kolekcji IHAR-PIB oraz wysokomorfinowa odmiana Lazur. Badania prowadzono w trzech sezonach wegetacyjnych. Zastosowano czternaście sposobów indukowania mutagenezy stosując zróżnicowane stężenia mutagenu chemicznego i ultradźwięków oraz różny czas ekspozycji mutagenem. Nasiona traktowano metanosulfonianem etylu (EMS) oraz ultradźwiękami (UD) w celu ułatwienia wnikania substancji mutagennej do nasion. Stężenia mutagenu wynosiły: 0,4; 0,6; 0,8; 1,0; 1,2; 1,4 i 1,6% EMS. Nasiona wytypowanych genotypów maku pozostawały w roztworze trzy lub cztery godziny w zależności od stężenia mutagenu. Przeprowadzono również badania wpływu ultradźwięków na zwiększenie działania mutagenezy chemicznej na nasiona maku odmiany Lazur. Czas ekspozycji nasion na działanie ultradźwięków wynosił 5 lub 25 minut. Po zdekantowaniu roztworu nasiona wymywano pod bieżącą wodą i osączano na bibule. Nasiona poddane mutagenezie i linie kontrolne rozmnażano w szkółkach selekcyjnych. Zastosowanie mutagenezy pozwoliło na uzyskanie zmienności zawartości morfiny, kodeiny i tebainy. Poszerzenie zmienności zawartości alkaloidów w makowinach maku potwierdza, że EMS jest wydajnym mutagenem chemicznym stosowanym do tworzenia nowych zmienności.
Collar rot, caused by Rhizoctonia solani Kühn, is one of the most severe fungal diseases of opium poppy. In this study, heritability, genetic advance and correlation for 10 agronomic, 1 physiological, 3 biochemical and 1 chemical traits with disease severity index (DSI) for collar rot were assessed in 35 accessions of opium poppy. Most of the economically important characters, like seed and capsule straw yield per plant, oil and protein content of seeds, peroxidase activity in leaves, morphine content of capsule straw and DSI for collar rot showed high heritability as well as genetic advance. Highly significant negative correlation between DSI and seed yield clearly shows that as the disease progresses in plants, seed yield declines, chiefly due to premature death of infected plants aswell as low seed and capsule setting in the survived population of susceptible plants. Similarly, a highly significant negative correlation between peroxidase activity and DSI indicated that marker-assisted selection of disease-resistant plants based on high peroxidase activity would be effective and survived susceptible plants could be removed from the population to stop further spread.
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