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1

A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

100%

Panicz R., Zych S., Grzesiak W.

Polish Journal of Natural Sciences

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2014

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tom 29

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nr 2

Nucleotide composition of both growth hormone variants of rainbow trout (Oncorhynchus mykiss) has been strongly preserved evolutionally what might suggest that any change within these sequences can have an influence on the functioning of the somatotropic axis. A 121 bp fragment that contained nearly the entire B intron was amplified by the polymerase chain reaction. PCR products were bidirectionally sequenced. PCR products were digested by TaiI according to manufacturer’s instructions and resulting DNA was subjected to electrophoresis. An analysis of the gene fragment for growth hormone 2 showed the presence of SNP, easily identifiable by means of digestion with TaiI restriction enzyme. Statistical analysis confirmed that homozygous GHBB fish were the longest (31.77 cm) and the heaviest (404.70 g) and were statistically significantly different (P ≤ 0.05) from heterozygous GHAB fish. Mean length of GHAA homozygous fish was insignificantly lower (30.06 cm) with mean body weight of 339.12 g than homozygotes GHBB.

2

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Association of alpha-A globin gene polymorphism with its expression level in racing pigeons

86%

Jedrzejczak-Silicka M., Dudaniec K., Dybus A.

Acta Scientiarum Polonorum. Zootechnica

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2019

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tom 18

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nr 1

3

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

72%

Kempter J., Panicz R., Makowska M., Metza M., Keszka S., Kielpinski M., Sadowski J.

Zeszyty Naukowe. Acta Biologica. Uniwersytet Szczeciński

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2011

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tom 18

Genetic variability of three sympatric crucian carp ( Carassius carassius ) populations from NW Poland was studied within a research project aimed at assessing the utility of those populations for stocking in inland waters. DNA samples were collected from 65 individuals. Restriction analysis was performed using 4 enzymes (HaeIII, HinfI, FspBI, TasI) of known restriction sites. The restriction pro files obtained were classified as belonging to a single haplotype (H-1). Selected DNA products were sequenced; the subsequent comparison made it possible to detect the presence of substi- tutions in the genome fragment analysed.

4

Diagnosis of Echinococcus infection in humans based on PCR−RFLP analysis

72%

Turkowicz M., Cielecka D., Gierczak A., Michalowicz B.

Wiadomości Parazytologiczne. Suplement

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2008

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tom 54

5

PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

72%

Przybylska A., Fiedler Z., Obrepalska-Steplowska A.

Journal of Plant Protection Research

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2016

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tom 56

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nr 1

Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.

6

Identyfication of Staphylococcus spp. strains isolated from milk samples of mastitic cows by PCR-RFLP analysis of gap gene

72%

Karakulska J., Sawicka I.

Advances in Agricultural Sciences

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2008

|

tom 12

7

Occurrence of atypical and new genotypes of Toxoplasma gondii in free-range chickens intended for human consumption in Brazil

72%

dos Santos Silva A. C., de Barros L. D., Barros V. M. C., de Alcantara A. M., Andrade M. R., Garcia J. L., Mota R. A., Porto W. J. N.

Acta Parasitologica

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2020

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tom 65

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nr 3

8

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

72%

Karakulska J., Pobucewicz A., Nawrotek P., Muszynska M., Furowicz A. J., Czernomysy-Furowicz D.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

9

Genetic similarity of Pseudomonas syringae pv.tomato strains showing various virulence

72%

Kozik E. U., Pulawska J., Sobiczewski P.

Journal of Plant Protection Research

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2006

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tom 46

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nr 4

325-334

Bacterial speck of tomato caused by Pseudomonas syringae pv. tomato appeared to be recently the most important disease on tomato in Poland. The genetic relationships among four Polish strains of race 0 P. syringae pv. tomato of different origin, isolated from tomato plants, were examined by RAPD and PCR-RFLP techniques. Amplification of bacterial DNA using 33 primers with RAPD technique showed, that similarity of strains expressed by the Nei-Li coefficient was very high (above 0.8). Next, the restriction analysis of amplified region ITS with the use of 5 endonucleases revealed, that profiles obtained from electrophoretic separation of DNA fragments were also very similar. On the basis of those analyses it was concluded that all strains tested constituted a closely related group. However, they showed various level of virulence as was demonstrated on the inoculated leaves of tomato plants growing in the greenhouse.

10

Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis

72%

Biradar S. S., Saravanan B. C., Tewari A. K., Sreekumar C., Sankar M., Sudhakar N. R.

Acta Parasitologica

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2014

|

tom 59

|

nr 4

PCR-RFLP and nucleotide sequencing based genotyping of Toxoplasma gondii Indian isolates (Izatnagar and Chennai isolates and Chennai clone) vis-a vis RH-IVRI strain was conducted by targeting GRA6 as genetic marker. The 791 bp GRA6 product was PCR amplified from the genomic DNA of different T. gondii Indian isolates, including the RH-IVRI strain. Tru1I restriction endonuclease based PCR-RFLP of GRA6 sequence produced polymorphic digestion pattern that discriminated the virulent RH-IVRI strain (as type I) from the moderately virulent local isolates as type III. The PCR amplicon of T. gondii GRA6 from RH-IVRI strain as well as from the local isolates were cloned in cloning vector and custom sequenced. The nucleotide and deduced amino acid sequences of T. gondii isolates were aligned with that of the type I, II and III strains (RH, BEVERLEY, ME49, C56, TONT and NED) available in public domain and analyzed in silico using MEGA version 4.0 software. Nucleotide sequencing and phylogenetic analysis of GRA6 marker from the Indian isolates revealed a close genetic relationship with type III strains of T. gondii. Further, detection of a single nucleotide polymorphism (SNP) at positions 162 and 171 of the GRA6 marker, established the lineage of Indian isolates as type III. This is the first report on characterization of T. gondii lineage as type III in selective chicken population of India based on PCR-RFLP and sequence analysis of GRA6 gene.

11

Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions

72%

Mikaeili F., Mathis A., Deplazes P., Mirhendi H., Barazesh A., Ebrahimi S., Kia E. B.

Acta Parasitologica

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2017

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tom 62

|

nr 3

12

Genotyping of Giardia duodenalis human isolates using PCR-RFLP in Zabol City, East of Iran

72%

Abedi M., Dabirzadeh M., Ghasemian M.

Annals of Parasitology

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2016

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tom 62

|

nr Suppl.

13

Analysis of molecular variability among isolates of Aspergillus flavus by PCR-RFLP of the ITS regions of rDNA

72%

Mohankumar M., Vijayasamundeeswari A., Karthikeyan M., Mathiyazhagan S., Paranidharan V., Velazhahan R.

Journal of Plant Protection Research

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2010

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tom 50

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nr 4

A total of seventeen isolates of Aspergillus flavus from maize were collected from different agro-ecological zones of Tamil Nadu, India. The isolates were tested for their ability to produce aflatoxin B1 (AFB1) in vitro by indirect competitive enzyme-linked immunosorbent assay (ELISA). The amount of AFB1 produced by the isolates of A. flavus ranged from 1.9 to 206.6 ng/ml. Among the various isolates of A. flavus, the isolate AFM46 produced the highest amount of AFB1. DNA was extracted from A. flavus isolates and their molecular variability was investigated by using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) regions of ribosomal DNA. PCR amplification with ITS1 and ITS4 primers resulted in the amplification of a product of approximately 600 bp. Digestion of the PCR products with the restriction enzymes EcoRI, HaeIII and TaqI produced fragments of different sizes. Analysis of the genetic coefficient matrix derived from the scores of RFLP profiles showed that minimum and maximum per cent similarities among the tested A. flavus strains ranged from 0 to 88%. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into five groups (group I–V) confirming the genetic diversity among the A. flavus isolates from maize.

14

Preliminary analysis of Plasmodium vivax genotypes isolated in Southeastern Turkey

72%

Doskaya A. D., Doskaya M., Caner A., Gul K., Nergiz S., Can H., Guruz Y.

Acta Parasitologica

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2015

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tom 60

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nr 2

15

PCR methods in meat species identification as a tool for the verification of regional and traditional meat products

58%

Spychaj A., Mozdziak P. E., Pospiech E.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2009

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tom 08

|

nr 2

5-20

In the times of industrial food production, regional and traditional food articles provide an attractive alternative for people looking for unforgettable sensory impressions. Regional or traditional food, commonly recognized as palatable and healthy, is also, for many consumers, a unique, sentimental journey back to tastes from childhood times. A gradual increase of demand for this type of food articles as well as relatively high prices of these products may generate among unscrupulous food manufacturers a number of improper production practices, e.g. replacement of a more expensive meat by a less expensive alternative. Species composition of meat products can be verified using chromatographic, immunological, electrophoretic, or genetic methods. One of the genetic methods applied in examining the authenticity of food composition, including meat and its products, is the polymerase chain reaction (PCR). This paper presents the most important techniques utilizing this technology to identify the origin of specific meat components constituting part of regional or traditional food articles. It was demonstrated that PCR techniques, in combination with species-specific primers, PCR-RFLP, PCR-SSCP and real-time PCR, allow identification of meat species occurring in-dependently or in mixtures with other meat species as well as meat subjected to thermal treatment or other technological processes in the course of industrial production. The only exception is the PCR-RAPD method that fails to identify meat species in the case of strong DNA degradation or in complex meat mixtures

16

Characterization of three species from the subfamily Leuciscinae (Pisces, Cyprinidae) using the nuclear ITS-1 rDNA spacer

58%

Kirtiklis L., Grzymkowska M., Boron A.

Folia Biologica

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2013

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tom 61

|

nr 1-2

17

Wystepowanie fitoplazm w drzewach i krzewach ozdobnych w Polsce

58%

Kaminska M., Sliwa H.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2006

|

tom 510

|

nr 1

255-261

W latach 1998-2005 prowadzono obserwacje i badania nad występowaniem fitoplazm w drzewach i krzewach ozdobnych w Polsce. Przedmiotem badań były krzewy z rodzaju Clematis sp., Magnolia spp. i Rosa sp. oraz drzewa z rodzaju Acer sp., Carpinus sp., Fagus sp., Fraxinus sp. i Quercus spp. wykazujące objawy chorobowe sugerujące porażenie przez fitoplazmy. Objawy te obejmowały zahamowanie wzrostu, zmiany w zabarwieniu a nawet nekrozę i deformację liści, proliferację i zamieranie pędów, czasem deformację i zielenienie lub brak kwiatów oraz zamieranie roślin. Do wykrywania i identyfikacji fitoplazm stosowano test biologiczny oparty na reakcji rośliny testowej Catharanthus roseus, zakażanej techniką szczepienia, oraz analizę PCR-RFLP. Stosując technikę szczepienia, fitoplazmy przeniesiono z chorych róży, magnolii i klonu na siewki C. roseus. Przy pomocy techniki łańcuchowej reakcji polimerazy (PCR) ze starterami amplifikującymi fragment 16Sr DNA fitoplazm, obecność fitoplazmatycznego DNA wykryto w roślinach Rosa spp., Magnolia spp., Clematis sp., Fraxinus excelsior, Carpinus sp. i Acer negundo oraz w eksperymentalnie zakażonych siewkach C. roseus. Analiza RFLP produktu amplifikacji z użyciem enzymów restrykcyjnych AluI, HhaII MseI i RsaI wykazała, że rośliny grabu, jesionu, klonu, magnolii, powojnika i róży były naturalnie porażone przez fitoplazmę z grupy żółtaczki astra (16SrI-B) - 'Ca. Phytoplasma asteris’. W niektórych roślinach róży i magnolii stwierdzono także fitoplazmę z grupy proliferacji jabłoni (16SrX-A) - ‘Ca. Phytoplasma mali’. W badanych roślinach fitoplazmy występowały nierównomiernie i w stosunkowo niskiej koncentracji, przez co mogły być wykrywalne dopiero w drugiej rundzie PCR. W roślinach buka i dębu, mimo wyraźnych symptomów, nie wykryto obecności fitoplazmatycznego DNA.

18

PCR jako narzędzie do identyfikacji i różnicowania drobnoustrojów

51%

Marciniak M., Robak M.

Acta Scientiarum Polonorum. Biotechnologia

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2012

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tom 11

|

nr 2

Bazując na łańcuchowej reakcji polimerazy (PCR), opracowano wiele technik, które są stosowane w kryminalistyce, medycynie, badaniach filogenetycznych oraz do identyfikacji i różnicowania organizmów. Wybór metody do rutynowego stosowania w danym laboratorium nie jest prosty i wymaga zaznajomienia się z wieloma technikami. Chcąc ułatwić wybór metody do analizy drobnoustrojów, w pracy zamieszczono opis i porównanie następujących technik: RAPD (Randomly Amplified Polymorphic DNA), PCR- RFLP (PCR-Restriction Fragments Length Polymorphism), RISA (Ribosomal Intergenic Spacer Analysis), AFLP (Amplified Fragment Length Polymorphism), Multiplex PCR oraz Real-time PCR. Metody porównano pod względem cech najbardziej użytecznych, jakimi są: zakres stosowania technik, powtarzalność analiz, trudność w wykonaniu oznaczeń, czasochłonność, możliwości automatyzacji badań oraz koszty jednostkowej analizy.

19

Identyfikacja mutacji BLAD u bydla metoda PCR-RFLP

51%

Natonek M.

Biuletyn Informacyjny. Instytut Zootechniki

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2000

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tom 38

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nr 4

29-33

20

Typowanie molekularne szczepow Campylobacter przy uzyciu technik opartych na reakcji lancuchowej polimerazy

44%

Wieczorek K., Osek J.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2008

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tom 07

|

nr 2

29-39

Ograniczanie wyników

A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

Association of alpha-A globin gene polymorphism with its expression level in racing pigeons

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

Diagnosis of Echinococcus infection in humans based on PCR−RFLP analysis

PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

Identyfication of Staphylococcus spp. strains isolated from milk samples of mastitic cows by PCR-RFLP analysis of gap gene

Occurrence of atypical and new genotypes of Toxoplasma gondii in free-range chickens intended for human consumption in Brazil

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Genetic similarity of Pseudomonas syringae pv.tomato strains showing various virulence

Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis

Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions

Genotyping of Giardia duodenalis human isolates using PCR-RFLP in Zabol City, East of Iran

Analysis of molecular variability among isolates of Aspergillus flavus by PCR-RFLP of the ITS regions of rDNA

Preliminary analysis of Plasmodium vivax genotypes isolated in Southeastern Turkey

PCR methods in meat species identification as a tool for the verification of regional and traditional meat products

Characterization of three species from the subfamily Leuciscinae (Pisces, Cyprinidae) using the nuclear ITS-1 rDNA spacer

Wystepowanie fitoplazm w drzewach i krzewach ozdobnych w Polsce

PCR jako narzędzie do identyfikacji i różnicowania drobnoustrojów

Identyfikacja mutacji BLAD u bydla metoda PCR-RFLP

Typowanie molekularne szczepow Campylobacter przy uzyciu technik opartych na reakcji lancuchowej polimerazy