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Nucleotide composition of both growth hormone variants of rainbow trout (Oncorhynchus mykiss) has been strongly preserved evolutionally what might suggest that any change within these sequences can have an influence on the functioning of the somatotropic axis. A 121 bp fragment that contained nearly the entire B intron was amplified by the polymerase chain reaction. PCR products were bidirectionally sequenced. PCR products were digested by TaiI according to manufacturer’s instructions and resulting DNA was subjected to electrophoresis. An analysis of the gene fragment for growth hormone 2 showed the presence of SNP, easily identifiable by means of digestion with TaiI restriction enzyme. Statistical analysis confirmed that homozygous GHBB fish were the longest (31.77 cm) and the heaviest (404.70 g) and were statistically significantly different (P ≤ 0.05) from heterozygous GHAB fish. Mean length of GHAA homozygous fish was insignificantly lower (30.06 cm) with mean body weight of 339.12 g than homozygotes GHBB.
The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.
Acid phosphatase (AcP) is a commonly observed enzyme in animal semen. In this study, AcP in rainbow trout (Oncorhynchus mykiss) spermatozoa was partly purified and characterized. Extraction in 0.85% NaCl with 0.1% Triton X-100 enabled obtaining 95% of total AcP activity observed in sperm supernatant. Kinetic characteristics were described for the enzyme from sperm extract and for the partly purified enzyme following gel filtration. The optimum pH was 5.8 for unpurified and 5.6 for partly purified enzyme. The affinity of the substrates measured in the sperm extract for p-nitrophenylphosphate dissodium salt and b-glycerophosphate was Km = 1.5 × 10-3M and Km = 1.9 × 10-3M, respectively. The Km for partly purified enzyme was similar at 1.67 × 10-3M measured with p-nitrophenylphosphate dissodium salt. L-tartaric acid and ammonium molybdate were the inhibitors of AcP for unpurified and partly purified enzyme. SDS-PAGE electrophoresis revealed that AcP from rainbow trout had a molecular weight of about 41 kDa.
The colour of light is a very important environmental factor that affects fish physiology. The aim of the present study was to evaluate the effects of different colours of light on weight gain, length, condition factor and specific growth rates of the juvenile rainbow trout Oncorhynchus mykiss. The study employed fertilized eggs that were exposed separately to seven colour lamps including white (573 nm, control), azure (397 nm), blue (459 nm), green (524 nm), yellow (586 nm), orange (611 nm) and red (742 nm). The experiment was conducted for 5 months (from fertilization until the fish reached 2 g wet weight) at the Sheshpir fish farm (west of Fars province), Iran. After yolk sac absorption, the weight and length of randomly sampled fish were measured monthly. The results showed that weight parameters of fish exposed to yellow (0.562 ± 0.13) and white (0.547 ± 0.13) coloured light were higher (p ≤ 0.05) than fish subjected to the other colours. The highest length growth was observed in fish exposed to yellow (3.91 ± 0.16) and white (3.61 ± 0.10) light, respectively. The highest growth rate (4.641 ± 0.29) and condition factor (2.00 ± 0.03) were observed in fish maintained under yellow light.
Chloramine-T is a widely used disinfectant for the treatment of gill diseases of fish in freshwater, and more recently attention has turned to its use in seawater. However, despite the wide use of chloramine-T, few studies have examined its toxicity to fish. Therefore, the aim of the current study was to examine the effects of disinfection by Chloramine-T on the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) using oxidative stress biomarkers (levels of 2-thiobarbituric acid reactive substances and derivatives of oxidatively modified proteins) and biochemical enzymes’ activity (alanine- and aspartate aminotransferases (ALT and AST), lactate dehydrogenase (LDH)) to observe the its toxic effects. The endpoints obtained from this study will be useful to monitor the effects of disinfectant bathing with Chloramine-T for this species of fish. In the disinfectant group, rainbow trout (n = 11) were exposed to Chloramine-T in final concentration of 9 mg per L. Control group of trout (n = 11) was handled with water from basin in the same way as Chloramine-T exposed group. Fish were bathed with Chloramine-T for 20 min and repeated three times every 3 days. Two days after the last bathing fish were sampled to study. Our results showed that Chloramine-T bathing caused the decrease of the lipid peroxidation as well as ALT and AST activity and significant decrease of LDH activity (by 339%, p = 0.017) compared to controls. Chloramine-T markedly affects on lactate and pyruvate metabolism and resulted to decrease of LDH activity. Correlative analysis revealed that the lipid peroxidation level is correlated with ALT and AST activity in the muscle tissue of unhandled control group. In the muscle tissue of trout disinfected by Chloramine-T, LDH activity is correlated positively with ALT and AST activity. Thus, the skeletal muscles of fish play an important role in the processing of lactate through the gluconeogenic and glycogenic pathways including a greater potential for biosynthesis. Our studies indicated that Chloramine-T in dose of 9 mg per L could at least partly attenuate oxidative stress and can be used for prophylactic disinfecting treatment of rainbow trout. Oxidative stress and biochemical alterations could be effectively used as potential biomarkers of Chloramine-T toxicity to the fish in the warning signal for pharmaceutical exposure to aquatic organisms. However, more detailed studies on using of these specific biomarkers to monitor the disinfectant treatment in aquaculture are needed.
Celem pracy była ocena wartości użytkowej i jakości mięsa pstrąga tęczowego w zależności od masy ryb. Badaniami objęto 18 pstrągów, odłowionych w gospodarstwie z terenu województwa lubelskiego, które podzielono na dwie grupy sortymentowe: S – do 350 g (n=10) i D – powyżej 350 g (n=8). Obie grupy ryb, pomimo istotnej różnicy w masie ciała, miały zbliżony udział mięsa w tuszy (51-52%). Mięso pstrągów większych (pow. 350 g) charakteryzowało się korzystniejszym składem chemicznym.
Oxygen uptake and carbon dioxide release by rainbow trout (Oncorhynchus mykiss Walb.) embryos exposed to constant magnetic fields of 5 and 10 mT (oxygen uptake and carbon dioxide release) and 50, 150, and 300 mT (oxygen uptake) were measured. The data were compared with those recorded in embryos developing under natural magnetic field (control). The magnetic fields tested were found to stimulate respiratory processes in the rainbow trout embryos as shown by a significantly increased oxygen consumption, particularly during periods of intensified morphogenesis. Exposure to 5 and 10 mT magnetic fields resulted in a slightly higher carbon dioxide release, the oxygen consumption being observed to increase as well. The respiratory quotient of the embryos exposed to magnetic fields was slightly higher than that in the control.
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