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An experiment was conducted to evaluate the growth performance of Nile tilapia fed with supplemental feed (25% crude protein) at 50% satiation. Juvenile Nile tilapia (average weight 6.4 g) were stocked in an earthen pond at 2.4 fish m⁻² . The pond was fertilized twice weekly with a mixture of superphosphate and urea fertilizer (68 kg ha 1⁻¹). The annual fish yield was 6880 kg ha⁻¹. The mean weight of harvested fish ranged from 110 to 250 g. The food conversion ratio (FCR) was 0.82, the average daily weight gain (ADG) was 0.9 g d⁻¹ and the specific growth rate was 1.55 % d⁻¹. This study demonstrated that 50% satiation feeding is an effective feeding rate for improving the production of Nile tilapia cultured in a fertilized, freshwater earthen pond.
Scale deformity in Nile tilapia, Oreochromis niloticus, (Actinopterygii: Cichlidae) is reported for the first time from Dongola, north of Sudan. The abnormal scales are fused pairs, with two well-developed foci. Developmental irregularity, probably caused by partially arrested growth, is believed to be the cause of such deformity.
Gyrodactylus malalai sp. nov. is described from the fin surface of cichlid fishes Oreochromis niloticus (L.) and Tilapia zillii (Gervais) caught in Lake Turkana (Kenya). The new species morphologically resembles Gyrodactylus nyanzae Paperna, 1973, but can be readily distinguished by the shape of the marginal hook sickles and the size of its hamuli. The sequence data of rDNA spanning partial 18S, internal transcribe spacer 1 and 2 and the 5.8S gene is unique within GenBank. Genetically, as most similar Gyrodactylus ergensi Přikrylová, Matějusová, Musilová et Gelnar, 2009 was found (97.5%). Moreover, a specimen of G. cichlidarum from O. niloticus, and a specimen G. ergensi from Sarotherodon galilaeus (L.) were collected during sampling in Kenya. Likewise, additional sampling of O. niloticus from the Blue Nile in Sudan revealed the presence of the newly described species. These findings represent the first records of gyrodactylids in both African countries.
Gyrodactylus infections in intensively-reared populations of Nile tilapia, Oreochromis niloticus niloticus, have been associated world-wide with high mortalities of juvenile fish. In this study, 26 populations of Gyrodactylus parasitising either O. n. niloticus or Mozambique tilapia, Oreochromis mossambicus, were sampled from fourteen countries and compared with type material of Gyrodactylus cichlidarum Paperna, 1968, Gyrodactylus niloticus (syn. of G. cichlidarum) and Gyrodactylus shariffi Cone, Arthur et Bondad-Reantaso, 1995. Representative specimens from each population were bisected, each half being used for morphological and molecular analyses. Principal component analyses (PCA) identified five distinct clusters: (1) a cluster representing G. cichlidarum collected from O. n. niloticus from 13 countries; (2) the G. shariffi paratype; (3) three specimens with pronounced ventral bar processes collected from two populations of Mexican O. n. niloticus (Gyrodactylus sp. 1); (4) four specimens collected from an Ethiopian population nominally identified as O. n. niloticus (Gyrodactylus sp. 2); (5) nine gyrodactylids from South African O. mossambicus (Gyrodactylus sp. 3). Molecular analyses comparing the sequence of the ribosomal transcribed spacer regions (ITS 1 and 2) and the 5.8S gene from the non-hook bearing half of worms representative for each population and for each cluster of parasites, confirmed the presence of G. cichlidarum in most samples analysed. Molecular data also confirmed that the DNA sequence of Gyrodactylus sp. 2 and Gyrodactylus sp. 3 (the morphologically-cryptic group of South African specimens from O. mossambicus) differed from that of G. cichlidarum and therefore represent new species; no sequences were obtained from Gyrodactylus sp. 1. The current study demonstrates that G. cichlidarum is the dominant species infecting O. n. niloticus, being found in 13 of the 15 countries sampled.
One previously described and 1 new species of Nanotrema Paperna, 1969 are reported from the gills of Citharinus citharus citharus (Citharinidae) collected from the Niokolo Koba River, Senegal. Nanotrema citharini Paperna, 1969 is redescribed, based on the re-examination of the type specimens and new information obtained from material taken in Senegal (new locality record). Nanotrema niokoloensis sp. nov. is distinguished primarily by possessing a copulatory organ composed of a long thin tube with median portion usually coiled into about 2.5 rings and an accessory piece resembling a braid lying within the rings. Based on the presence of the dorsal anchors modified into paired haptoral spikes, which lack an associated bar, species of Nanotrema phenotypically appear to be related to those of Neotropical Rhinoxenus Kritsky, Boeger et Thatcher, 1988 and Indian Spicocleidus Agrawal, Tripathi et Shukla, 2005.
Background. Increasing global usage of inorganic fertilizers, including phosphate-based fertilizers has its negative consequences on the aquatic environment. Effects of single superphosphate fertilizer (SPF) remain unknown, particularly its influence on the respiratory dynamics of fish under continuous exposure. We investigated the effects of single SPF on the survival and respiratory dynamics of Nile tilapia, Oreochromis niloticus, under laboratory conditions. Materials and Methods. Nile tilapia fingerlings (of mixed sex) (5.40 . 0.03 g) were exposed to various concentrations of the fertilizer in five treatment regimes (in triplicate): 0.88, 1.75, 3.50, 7.00, 14.00 g . L–1 (and 0.00 g . L–1 for control). Each replicate was carried out in a 30-L circular plastic tank based on 20 fingerlings. The study involved: the mortality estimation, the oxygen consumption, the histopathological effects on fish gills, and the activities of lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH) in liver of fish exposed to sublethal concentrations (0.44, 0.22, 0.11, 0.06, and 0.03 g . L–1) of single SPF for eight weeks under laboratory conditions. Results. Acute concentrations of SPF had serious adverse effects on mortality, oxygen consumption and opercular ventilation rates of exposed fish. All variables showed a dose-dependency. A mean value of 96-h LC50 of the SPF to the test fish was calculated to be 3.76 g . L–1. At various acute concentrations, oedema and hyperplasia of gill lamellae were observed in exposed fish. Exposure of the fish to sublethal concentrations of the SPF resulted in reduction in the levels of lactate dehydrogenase and alcohol dehydrogenase activities in liver. Conclusion. Concentrations of SPF in natural water bodies are deleterious to aquatic fauna. With rapid global economic development and need for more food production, pollution from agricultural fertilizers remains a major threat to the aquatic ecosystem. Therefore it is ultimately important that a balance is struck between achieving economic excellence and environmental protection through good pollution management strategies.
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