Wyniki wyszukiwania

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Neospora caninum - vertical transmission in dairy cattle

100%

Cabaj W., Moskwa B., Choromanski L., Rodgers S., Pastusiak K., Sloniewski K.

Wiadomości Parazytologiczne. Suplement

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2001

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tom 47

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nr 2

2

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Sprawozdanie z konferencji naukowo-szkoleniowej pt.: Neospora and neosporosis: Achievements and Perspectives

88%

Moskwa B.

Wiadomości Parazytologiczne

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2005

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tom 51

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nr 4

311-312

3

Is the immune response to Neospora caninum incompatible with pregnancy in cattle?

75%

Rosbottom A., Smith R. F., Kipar A., Guy C. S., Gibney H., Kaiser P., Bainbridge D. R. J., Valarcher J. F., Taylor G., Trees A. J., Williams D. J. L.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

4

The occurrence of protozoan parasites Neospora caninum and Toxoplasma gondii in raccoons (Procyon lotor)

75%

Kornacka A., Moskwa B.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

5

Neospora caninum natural infection in Tunisian rams: serological study and molecular identification of infection in semen

75%

Amdouni Y., Rouatbi M., Lassoued N., Rekik M., Gharbi M.

Acta Parasitologica

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2019

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tom 64

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nr 4

6

Ovine abortion by Neospora caninum: first case reported in Argentina

75%

Hecker Y. P., Morrell E. L., Fiorentino M. A., Gual I., Rivera E., Fiorani F., Dorsch M. A., Gos M. L., Pardini L. L., Scioli M. V., Magarinos S., Paolicchi F. A., Canton G. J., Moore D. P.

Acta Parasitologica

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2019

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tom 64

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nr 4

7

Proba izolacji, identyfikacji oraz hodowli pierwotniaka Neospora canium

75%

Pastusiak K.

Wiadomości Parazytologiczne

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2004

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tom 50

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nr 4

763-764

8

Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in Southern Romania

75%

Enachescu V., Ionita M., Mitrea I. L.

Acta Parasitologica

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2014

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tom 59

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nr 1

The study aimed to assess the within-herd Neospora caninum exposure in dairy cattle in southern Romania, based on the detection of specific antibodies in milk and serum. A total of 104 paired samples of milk and serum were collected from four dairy farms. Individual samples were analyzed for N. caninum antibodies by ELISA: IDEXX Neospora Ab (Idx) (three farms: A, B, C; n = 60) and ID-VET Lab (Idv) (farm D; n = 44). Additionally, four pooled milk samples, one per each farm (A, B, C) and a composed one (A+B+C), were analyzed with Idx ELISA. Optimized cut-off values for milk samples were determined by receiver operating characteristic (ROC) analysis, with serum results considered as true status. The agreement was expressed by K values. The overall seroprevalence of N. caninum infection was 45% in the farms tested by Idx ELISA and 56.8% in the farm tested by Idv ELISA. A good agreement between serum and milk was obtained for both ELISA kits (K = 0.72 and 0.77, respectively). The specificity and sensitivity at optimized cut-off of S/P>0.704 for Idx and S/P%>7.966% for Idv were 100% and 70.37% for Idx and 89.47% and 88% for Idv. Testing pooled milk samples, there were identified as N. caninum positive the dairy farms with a 15% or higher within-herd seroprevalence at the cut-off value of S/P>0.51. This is the first study in Romania in which milk samples were tested to determine the N. caninum infection status in dairy farms, providing a base for further researches.

9

PCR detection of Neospora caninum in water buffalo foetal tissues

75%

Auriemma C., Lucibelli M. G., Borriello G., De Carlo E., Martucciello A., Schiavo L., Gallo A., Bove F., Corrado F., Girardi S., Amoroso M. G., Uberti B. D., Galiero G.

Acta Parasitologica

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2014

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tom 59

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nr 1

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

10

Detection of antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.)

75%

Cabaj W., Moskwa B., Pastusiak K., Bien J.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

11

Use of a simplified method to determine the avidity of an IgG antibody response against the p38 antigen of Neospora caninum in cattle

75%

Schares G., Staubach C., Bjorkman C., Conraths F. J.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

12

Seroprevalence of Neospora caninum infection in dog population worldwide: a systematic review and meta-analysis

75%

Anvari D., Saberi R., Sharif M., Sarvi S., Hosseini S. A., Moosazadeh M., Hosseininejad Z., Chegeni T. N., Daryani A.

Acta Parasitologica

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2020

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tom 65

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nr 2

13

Neospora caninum infections in aborting dairy cows in Poland

75%

Cabaj W., Choromanski L., Rodgers S., Moskwa B., Malczewski A.

Acta Parasitologica

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2000

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tom 45

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nr 2

Antibodies to the coccidian Neospora caninum were measured in serum samples derived from dairy cows in Poland that had aborted in 1998-2000, using an ELISA. Antibodies to N. caninum were found in 7 of 45 (15.6%) cows. The presence of specific antibodies in 15.6% of the tested animals indicates that the aborted cows were exposed to N. caninum and neosporosis should be considered in differential diagnosis of bovine abortion in Poland.

14

Diagnosis of bovine neosporosis: Recent advances and perspectives

75%

Ortega-Mora L. M., Fernandez-Garcia A., Gomez-Bautista M.

Acta Parasitologica

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2006

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tom 51

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nr 1

Neospora caninum is considered a major cause of abortion in cattle. Appropriate techniques for diagnosis of bovine neosporosis, both in vivo and in aborted foetuses, have been developed in the last ten years and some of them are commercially available. For diagnosis in live animals, detection of antibodies in serum or milk has been shown to be the best option both at the herd and the individual level. These techniques are excellent tools to examine N. caninum-associated abortion problems and to adopt some basic herd-control measures. Concerning foetal diagnosis, detection of compatible lesions by histological examination and parasites by PCR in brain (as well as heart and liver) are the best choices. Diagnostic criteria to distinguish foetal infection and Neospora-associated abortion are based not only on the demonstration of the parasite in the foetus but also on the extent and severity of the lesions in the foetus, foetal age and the assessment of neosporosis at the herd level. In the near future, new tools to diagnose infection should help to detect animals with parasite reactivation by testing the immune response to stage-specific antigens and lead to the development of molecular typing methods to characterise different parasite isolates. Finally, uniform diagnostic procedures need to be established between laboratories and countries in order to standardise result interpretation. The role of National or Regional Reference Laboratories is essential in countries or regions where control programmes for the disease are being developed.

15

Seroprevalence of Neospora caninum in racehorses in Ankara, Turkey

75%

Kilbas Z. G., Adanir R., Avcioglu H.

Acta Parasitologica

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2008

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tom 53

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nr 3

To assess seroprevalence of Neospora spp. in asymptomatic horses in Ankara, Turkey, 19 mares and 56 stallions older than 2 years of age were examined using ELISA; 9.3% of the horses were seropositive. Seroprevalence of N. caninum in mares was twice of that in stallions (15.8 vs 7.1%) and all appeared to be asymptomatic.

16

Neonatal Neospora caninum infections in dogs

75%

Dubey J. P., Knickman E., Greene C. E.

Acta Parasitologica

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2005

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tom 50

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nr 2

Neosporosis was diagnosed ante-mortem in a litter of neonatally-infected dogs. Three pups developed weakness of limbs 7-9 weeks after birth. One of the dogs developed megaesophagus. Treatment with clindamycin improved clinical signs but did not eradicate the parasite. All 3 dogs were euthanized and viable N. caninum was isolated from the brains of all 3 dogs. Tissue cysts were found in the brain and muscles of dogs. The dam was bred again. Seven apparently healthy pups were born in the second litter. Six of the 7 pups from the second litter had no demonstrable N. caninum antibodies at 32 day of age. The seventh pup had high (1:1280) titer in the Neospora agglutination test and the titer remained stable at day 227 when the study was discontinued. The results suggest that the rate of congenital transmission of N. caninum decreased in the subsequent pregnancy.

17

Ocena wybranych parametrow biochemicznych w surowicy jako potencjalnych wyznacznikow klinicznych zarazenia bydla mlecznego Neospora caninum

63%

Moskwa B., Cabaj W., Wojdan J.

Folia Universitatis Agriculturae Stetinensis. Zootechnica

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2001

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nr 42

121-125

18

Seroepidemiological study of Neospora caninum in beef and dairy cattle in La Pampa, Argentina

63%

Fort M., Edelsten M., Maley S., Innes E.

Acta Parasitologica

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2015

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tom 60

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nr 2

19

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

63%

Garcia-Melo D. P., Regidor-Cerrillo J., Ortega-Mora L. M., Collantes-Fernandez E., Ferreira de Oliveira V. S., Pelli de Oliveira M. A., Caetano da Silva A.

Acta Parasitologica

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2009

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tom 54

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nr 2

180-185

Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

20

Evaluation of frequency of antibodies against Toxoplasma gondii, Neospora caninum and Sarcocystis spp. and transmission routes in sheep from Humid Pampa, Argentina

63%

Hecker Y. P., Masson F. M., Armendano J. I., Cora J., Olivares C. F., Gual I., Pardini L., Moore D. P., More G., Canton G. J.

Acta Parasitologica

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2018

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tom 63

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nr 2

Ograniczanie wyników

Neospora caninum - vertical transmission in dairy cattle

Sprawozdanie z konferencji naukowo-szkoleniowej pt.: Neospora and neosporosis: Achievements and Perspectives

Is the immune response to Neospora caninum incompatible with pregnancy in cattle?