The article concentrates on diagnostic aspects of the major mycoplasma diseases in ruminants. Among them particular attention is given to: bovine contagious pleuropneumonia and contagious agalactia of sheep and goats, and an involvement of mycoplasmal etiological agent in bovine respiratory disease (BRD). The diagnosis of mycoplasma infections is based on serological methods such as: Elisa test, complement fixation test (CFT), and Western blot. The culture and identification of Mycoplasma spp. are possible through the use of specific liquid or solid Eaton’s media. The doubtful cases are confirmed by molecular biology methods (PCR and DGGE).
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