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Three 1 year old sheep were infected per os with 1000, 2000, and 13000 invasive larvae of Elaphostrongylus cervi. Animals did not show any clinical symptoms of invasion. No larvae of this nematode were found in the samples of faeces during the experiment. During post mortem examination of infected sheeps no parasites or their larvae were found in tissues, and no pathological lesions caused by parasites were observed.
Faeces of red deers from Białowieża Forest containing first stage larvae of Elaphostrongylus cervi have been stored during live years in -20°C. After this time 52% of the larvae survived. These larvae were fully alive and in experimentally infected snails Helix pomatia developed to the infectious stage.
Angiostrongylus vasorum belongs to the superfamily of Metastrongyloidea. This nematode occurs in foxes, dogs and other predators. The Nematode A. vasorum place themselves in the pulmonary artery and its branches, and in the right ventricle and atrium of the heart. Numerous species of land snails are the intermediate hosts of the parasite. In 2013, lungs and hearts of 76 foxes shot in the Forest District Głęboki Bród in Augustowska Primeval Forest were parasitologically necropsied. Four of the examined foxes were infected with the nematode A. vasorum, a prevalence of 5.2%. In one fox pericardium there were 6 male and 6 female nematodes. In the remaining three foxes nematodes were localized in the pulmonary artery. In two foxes 2 specimens of nematodes were detected (male and female, and two females) while 1 female was detected in the other fox. This is the first report of the presence of the nematode A.vasorum in fox in Poland.
The objective of the study was to establish possibilities of the development of the I stage larvae of E. cervi in fishes and amphibians, and possibilities of paratheny in the organism of these animals. In the alimentary tract of the fishes both the I stage and the invasive (i.e. III stage) larvae can not cross the intestine barrier and undergo digestion. However, in the frag organism the I stage larvae of E. cervi are capable of crossing the wall of the alimentary canal and of going through a part of their development, though not attaining the II stage. The invasive larvae fed to the frogs penetrate under the peritoneum, into the mesenterium and the muscles and there they survive inside cysts. Frogs as parathenic hosts for E. cervi can be dangerous for predators feeding on amphibians. The larvae of E. cervi liberated in their organism can pass through the cerebral phase of their development thus causing neural clinical symptoms.
Faecal samples from 716 cats from Poland, were examined for lungworm larvae (Metastrongyloidea) by flotation, sedimentation and Baermann techniques between January 2016 and April 2019 year. In total, 1.1% (8/716; CI ± 95% 0.3-1.9) of cats shed lungworm larvae. In the animals examined, infections with Aelurostrongylus abstrusus predominated (7 positive samples), while larvae of Troglostrongylus brevior were detected in only one sample. These invasions were more prevalent in animals under 6 months of age (3.0%) (6/201; CI ± 95%; 0.6-5.4) than in those older than one year (0.4%) (2/515; CI ± 95% 0.0-0.9). Overall, a distinct seasonal pattern was apparent in the detection of infected cats, but annual volatility was not observed. A relatively large proportion of lungworm-infected cats (4 out of 8) had pulmonary syndromes, which indicates that these parasitic diseases should be considered in differential diagnosis of any inflammations of the respiratory system, especially in young animals.
Single doses (from 300 to 1000 larvi per an animal) of invasive larvae E. cervi Cameron, 1931, obtained from experimentally infected snails Helix pomatia L. were given to 17 guinea pigs and 17 golden hamsters. Clinical nervous symptoms in the form of paresis and paralysis of limbs occurred only in the guinea pigs which were given a dose of 1000 larvi. These animals died in the period from the 75th to 117th day of infection. From their central nervous system single adult males and females of E. cervi were isolated. In the lungs and mesenteries of 2 dead pigs live larvae of E. cervi were found. This fact proves that the guinea pig can fulfil the role of a final and a paratenic host of E. cervi. No clinical symptoms were noticed in any hamster. In hamsters dissected on the 7th day of infection live larvae of E. cervi were found in the mesentery and in the fleshy part of the diaphgram. After 14 days the larvae found both in the mesentery and in the diaphragm were dead and surrounded by cellular infiltration. A strong tissue reaction of the hamster after the administration of E. cervi larvae is responsible for the larvae destruction and resorption.
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