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Somatic embryogenesis in callus cultures of tomato Lycopersicon peruvianum (L.) Mill.

100%

Rzepka-Plevnes D., Kulpa D., Kurek J.

Zeszyty Problemowe Postępów Nauk Rolniczych

2007

tom 523

Screening of cultivated and wild Lycopersicon species for tomato spotted wilt virus [TSWV]

84%

Rusinowski Z., Kaminska M., Kozik E., Niemirowicz-Szczytt K.

Journal of Applied Genetics

1996

tom 37A

Comparison of morphogenetic potential of protoplasts isolated from leaf mesophyll of Lycopersicon glandulosum, L.peruvianum, L.esculentum cv.Stevens Rolade

67%

Pindel A., Lech M., Miczynski K.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

1997

tom 39

nr 1

Regeneration of leaf mesophyll protoplasts from Lycopersicon glandulosum, L. peruvianum and L. esculentum Stevens x Rodade hybrid

67%

Pindel A., Lech M., Miczynski K.

Acta Biologica Cracoviensia. Series Botanica

1998

tom 40

Protoplasts were isolated from mesophyll leaf tissue of in-vitro-grown 3-4-week-old seedlings of two wild tomato species L. glandulosum C.H. Mull. PI 731163 and L. peruvianum (L.) Mill. PI 128650, and from F4 plants of the Stevens x Rodade hybrid of L. esculentum Mill. For protoplasts isolation from both wild species a mixture of 0.3% cellulase + 0.03% macerozyme + 0.01% MES and 7.0% sorbitol was used, whereas maceration of leaf pieces from Stevens x Rodade was performed in a mixture of 0.3—1.0% cellulase + 0.2% macerozyme + 0.01% MES + 0.05% glycine and 7.0% sorbitol. Protoplast culture was carried out in a slightly modified nutrient medium of Tan et. al. (1987b). The yield of protoplasts per 1 g tissue amounted to 9 x 107 for both wild species, and 4 x 108 for the Stevens x Rodade plants. The first cell divisions of protoplasts from all three studied species occurred after 6-10 days of culture. Regenerated shoots rooted in 90% of both wild Lycopersicon species. Regeneration of Stevens x Rodade did not pass the greening callus stage.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

1 Journal of Applied Genetics

1 Zeszyty Problemowe Postępów Nauk Rolniczych

2 Lech M.

2 Miczynski K.

2 Pindel A.

1 Kaminska M.

1 Kozik E.

1 Kulpa D.

1 Kurek J.

1 Niemirowicz-Szczytt K.

1 Rusinowski Z.

1 Rzepka-Plevnes D.

1 2007

1 1998

1 1997

1 1996

Wyniki wyszukiwania

Somatic embryogenesis in callus cultures of tomato Lycopersicon peruvianum (L.) Mill.

Screening of cultivated and wild Lycopersicon species for tomato spotted wilt virus [TSWV]

Comparison of morphogenetic potential of protoplasts isolated from leaf mesophyll of Lycopersicon glandulosum, L.peruvianum, L.esculentum cv.Stevens Rolade

Regeneration of leaf mesophyll protoplasts from Lycopersicon glandulosum, L. peruvianum and L. esculentum Stevens x Rodade hybrid