High yields of viable protoplasts were obtained from the mesophyll leaf tissue of young seedlings of Lycopersicon glandulosum grown in vitro at 80 µmol s-1m-2 irradiance and 23°-24°C. The isolated protoplasts were cultured first on a modified semiliquid medium of Tan et al. (1987 b), on which they formed microcalli, and afterwards on a greening medium consisting of MS micro/macro nutrients, Nitsch vitamins and 0.5 mg/l BAP + 0.05 mg/l NAA. The green budding calli were in turn transferred to the same medium with BAP and NAA replaced by 2.0 mg/l zeatin + 0.1 mg/l IAA, on which they developed shoots. The regenerated shoots were rooted in 1/3 MS medium without growth regulators, and subsequently planted into pots in the greenhouse. The whole process of regeneration from the moment of protoplast isolation up to the flowering of regenerated plants lasted ~6 months. The regenerated tomatoes were morphologically identical to their parental source plants.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.