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Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions

100%

Skibinska M., Kosmala A., Humphreys M. W., Zwierzykowski Z.

Cellular and Molecular Biology Letters

2002

tom 07

nr 2A

At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid - L. multiflorum (4x) x F. pratensis (2x) and pentaploid - F. arundinacea (6x) x L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented.

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Meiosis and fertility of reciprocal triploid hybrids of Lolium multiflorum with Festuca pratensis

100%

Naganowska B., Zwierzykowski Z., Zwierzykowska E.

Journal of Applied Genetics

2001

tom 42

nr 3

The effect of drought stress on chlorophyll fluorescence in Lolium-Festuca hybrids

100%

Koscielniak J., Filek W., Biesaga-Koscielniak J.

Acta Physiologiae Plantarum

2006

tom 28

nr 2

The effects of drought on photochemical efficiency of PSII in leaves of 22 hybrids of Festuca pratensis xLolium multiflorum and Festuca pratensis x Loliumperenne and of Festuca pratensis cv. Skra were investigated. A significant decrease of electron transport efficiency (about 25 %) in PSII (FPSII) was not found before 9 days of seedling growth in hydroponics with water potential (Yw) equal to-0.8 MPa (simulated “soil drought”). The decrease of FPSII was similarly related to that of exciialion energy capiure by open PSII reaction centre (Fv’/Fm’) and also to the decrease of the proportion of oxidized to reduced QA (photochemical fluorescence quenching, qp). According to the drought prolongation, variation of all parameters of fluorescence between genotypes significantly increased. The seedlings of some genotypes were able to recover electron transport efficiency in PSII after increasing water potential in nutrient solution (removing the “soil drought”). When plants grew in containers with soil and 4 genotypes with the highest sensitivity of electron transport to drought (S) as well as 4 genotypes with the highest tolerance (T) were compared 17 days after watering ceased, Yw in leaves considerably decreased, but the differences between S and T genotypes were often not significant in this respect. The differences between S and T genotypes, as values of Fv/Fm were concerned, also appeared small (about 5 %), similarly as that of Fv’/Fm’ (5 %), qp (12 %) and FPSII (about 15 %). Drought stress increased non-photochemical quenching of chlorophyll fluorescence (NPQ) 15to47% and this could protect the PSII reaction centres from damages because of energy excess. The increase of NPQ was not closely connected with drought resistance of plants because it was similar in some genotypes tolerant to dehydration as well as in sensitive ones. The results of the experiments suggest that resources of genetic variability in Festulolium may be sufficient for revealing differences between genoiypes on the basis of measurement of chlorophyll a fluorescence, as far as their tolerance to soil drought is concerned. As the tolerance of PSII against drought is high, the determinations of fluorescence should be peri formed rather under severe stress. Such methods seem to be useful for selection of genotypes with high drought tolerance as well as with the ability to at least partial repairing of PSII after drought.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

2 Zwierzykowski Z.

1 Biesaga-Koscielniak J.

1 Filek W.

1 Humphreys M. W.

1 Koscielniak J.

1 Kosmala A.

1 Naganowska B.

1 Skibinska M.

1 Zwierzykowska E.

1 2006

1 2002

1 2001

Wyniki wyszukiwania

Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions

Meiosis and fertility of reciprocal triploid hybrids of Lolium multiflorum with Festuca pratensis

The effect of drought stress on chlorophyll fluorescence in Lolium-Festuca hybrids