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1

Mathematical modelling of life cycle and molecular detection of Leptosphaeria maculans and L. biglobosa species

100%
Dawidziuk A.
Phytopathologia
|
2011
|
tom 62
2

Epidemiology and damage caused by stem canker of oilseed rape in Poland

100%
Jedryczka M.
Phytopathologia Polonica
|
2007
|
tom 45
3

Powstrzymać zgniliznę

100%
Kalinowski M.
Tygodnik Poradnik Rolniczy
|
2013
|
nr 39
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Pseudothecial maturation and ascospore release of Leptosphaeria maculans and L. biglobosa in south-east Poland

100%
Dawidziuk A., Kasprzyk I., Kaczmarek J., Jedryczka M.
Acta Agrobotanica
|
2010
|
tom 63
|
nr 1
Stem canker of brassicas is a severe disease of oilseed rape in Australia, Canada and Europe, including Poland. The disease is caused by Leptosphaeria maculans and L. biglobosa - two pathogens belonging to the class Dothideomycetes. The species differ in pathogenicity, but they have identical shape and size of fruiting bodies and spores of the generative and vegetative stages. Both pathogens are often found together in infected tissues of oilseed rape plants. The main goal of the experiments was to measure the rate of pseudothecial maturation and to monitor ascospore concentration of L. maculans and L. biglobosa in air samples. The paper is the first investigation on the generative stage development of these two species in south-east Poland. The studies were done for three consecutive years (2005-2007), for six most important months in pathogen development and plant infection, including 3 months in the spring (March – May) and 3 months in the autumn (September – November). The stage of pseudothecial maturation was assessed visually, based on the development of asci and ascospores. Monitoring of spore concentration in the air was performed using a Hirst-type 7-day volumetric trap. It was proved that differences in pseudothecial maturation rate in south-east Poland, encompassing the climatic regions of the Carpathian Foothills and Cracow, do not exceed two weeks within one season. The first and the highest ascospore concentration dates depended on weather conditions in a particular season. The total number of spores during the studied seasons varied from 9 to12 spores/m3, which was from 70 to 90 times lower than the average from five other monitoring sites around Poland. The short exposition to spore showers and very small concentrations of L. maculans and L. biglobosa ascospores in air samples were the most probable reasons for relatively small damage of oilseed rape crops by stem canker in the south-east part of Poland.
5

Porownanie fungistatycznego oddzialywania bakterii propionowych i wybranych fungicydow wobec Leptosphaeria maculans i Leptosphaeria biglobosa

99%
Gwiazdowski R., Gwiazdowska D.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 2
101-104
6

Wpływ jesiennych i wiosennych zabiegów fungicydowych na porażenie roślin rzepaku ozimego przez grzyby powodujące suchą zgniliznę kapustnych

85%
Kaczmarek J., Maczynska A., Glazek M., Jedryczka M.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 2
Modern decision support systems for disease control – important elements of integrated plant protection – should recommend optimal time of fungicide spray. The aim of this study was to compare the efficiency of autumn and spring treatments in relation to the concentration of Leptosphaeria maculans and L. biglobosa ascospores in air samples. The experiment was done over two seasons in two climatic regions of Poland. In three out of four field situations the fungicide treatment following maximum ascospore release was more efficient than the early spring spray. However, the latter treatment was more efficient in the case of very late ascospore release (end of November), observed in southern Poland in the autumn of 2006. The increase of the seed yield correlated with the time of fungicide application and was on average 16.1% in the case of autumn spray following maximum ascospore release and only 6.23% when treatment was done in the spring.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Identyfikacja i porownanie patogenicznosci gatunkow Leptosphaeria maculans [Desm.] Ces. et de Not. i Leptosphaeria biglobosa [Shoemaker i Brun] po inokulacji rzepaku ozimego w warunkach polowych i laboratoryjnych

85%
Starzycki M., Starzycka E., Jedryczka M., Irzykowski W., Pszczola J., Solecka D.
Rośliny Oleiste - Oilseed Crops
|
2006
|
tom 27
|
nr 1
51-62
8

Epidemiologia i szkodliwosc suchej zgnilizny kapustnych na rzepaku ozimym w Polsce

80%
Jedryczka M.
Rozprawy i Monografie. Instytut Genetyki Roślin Polskiej Akademii Nauk
|
2006
|
tom 17
1-150
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Characterization of two coexisting pathogen populations of Leptosphaeria spp., the cause of stem canker of brassicas

80%
Kaczmarek J., Jedryczka M.
Acta Agrobotanica
|
2011
|
tom 64
|
nr 2
Stem canker of brassicas, also known as blackleg is the most damaging disease of many Brassicaceae. The disease is caused by Leptosphaeria maculans (Desm.) Ces et de Not. and L. biglobosa sp. nov., Shoemaker & Brun, which coexist in plants and resulting in disease symptoms and decreased yield, quantity and quality of cultivated vegetables and oilseed rape. The paper presents taxonomic relationships between these coexisting pathogen species, describes particular stages of their life cycles, summarizes the differences between the species, and reviews methods for their identification.
10

Geny chroniące przed fomą

80%
Kalinowski M.
Tygodnik Poradnik Rolniczy
|
2019
|
nr 29 [791]
11

The effect of adjuvants, spray volume and nozzle type on azoxystrobin efficacy against Leptosphaeria maculans and L. biglobosa on winter oilseed rape

80%
Ratajkiewicz H., Kierzek R., Karolewski Z., Wachowiak M.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 4
440-445
In two vegetative seasons 2005-2006 azoxystrobin (Amistar 250 SC at the dose of 0.7 dm3/ha) was applied as a spraying treatment at the beginning of plant flowering against stem canker (Leptosphaeria maculans and L. biglobosa) in winter oilseed rape crops. The effect of the following spray application parameters: water volume (200 and 400 l/ha), adjuvant type (Break Thru S 240 - 0.1% and Atpolan 80 EC - 0.5%), and nozzle type (XR11002 - fine droplet size and DB11002 - coarse droplets at 0.4 MPa pressure) on the fungicide efficacy was studied. The results of presented studies indicated that azoxystrobin (Amistar 250 SC) caused a significant decrease of rape infection by stem canker. Addition of adjuvants did not significantly increase the fungicide effectiveness, and sometimes lowered its action (especially after the application of Atpolan 80 EC). The applied spray volumes of water and nozzle type provided an effective protection of oilseed rape against stem canker, however, in some cases the results of field experiments were not significantly different. The best results of the disease casual agent (L. biglobosa) control, statistically proved were shown at the higher spray volume (400 l/ha), regardless of nozzle type (droplet size) and adjuvant application.
12

Tilmor - klucz do twojego sukcesu

80%
Top Agrar Polska
|
2012
|
nr 03
13

The maturation rate of the generative stage of Leptosphaeria maculans and Leptosphaeria biglobosa in central and eastern Poland

80%
Dawidziuk A., Podlesna A., Kaczmarek J., Jedryczka M.
Polish Journal of Agronomy
|
2010
|
nr 02
14

Effect of adjuvants, spray volume and nozzle type on metconazole activity against Leptosphaeria biglobosa and L. maculans during late spring treatments in winter oilseed rape

80%
Karolewski Z., Wachowiak M., Ratajkiewicz H., Kierzek R.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 1
113-117
In two seasons 2005-2006 the metconazole (Caramba 60 SL at the dose of 0.75 l/ha) was applicated by spraying at the beginning of flowering against phoma stem canker (Leptosphaeria biglobosa and L. maculans) in winter oilseed rape. The effect of water volume (200 and 400 l/ha), adjuvant type (Break Thru S 240 — 0.1% and Atpolan 80 EC — 0.5%), and nozzle type (XR11002 — fine droplet size and DB11002 — course droplets at pressure 0.4 MPa) on the biological efficacy of fungicide spraying was investigated in the studies. The results showed that adjuvants did not significantly influence biological efficacy of fungicide treatments against L. biglobosa and L. maculans. Generally, control of both pathogens on the leaves and stem did not depend on spray characteristics (nozzle types-droplet size). However, positive effect of air induction nozzles DB 11002 (course spray quality) on fungicide treatments against L. biglobosa, particularly with addition of adjuvant Atpolan 80 EC was observed. Two different volumes of water tested (200 and 400 l/ha) did not influence efficacy of metconazole in control L. maculans and L. biglobosa. The above suggests a possibility of decrease the volume of water used with fungicide to 200 l/ha, without a negative biological effects on fungicidal activity.
15

Silny atak suchej zgnilizny

80%
Jedryczka M., Kaczmarek J.
Top Agrar Polska
|
2011
|
nr 07
16

Sucha zgnilizna kapustnych

80%
Korbas M., Jajor E.
Farmer
|
2013
|
nr 03
17

Groźne choroby - sucha zgnilizna kapustnych

80%
Kalinowski M.
Tygodnik Poradnik Rolniczy
|
2020
|
nr 08 [822]
18

Superwarunki dla suchej zgnilizny kapustnych

80%
Kalinowski M.
Tygodnik Poradnik Rolniczy
|
2020
|
nr 11 [825]
19

Sucha zgnilizna kapustnych grozna dla rzepaku

80%
Jajor E., Korbas M.
Agrotechnika. Poradnik Rolnika
|
2007
|
nr 09
24-27
20

Analyses of air samples for ascospores of Leptosphaeria maculans and L. biglobosa by light microscopy and molecular techniques

80%
Kaczmarek J., Jedryczka M., Fitt B. D. L., Lucas J. A., Latunde-Dada A. O.
Journal of Applied Genetics
|
2009
|
tom 50
|
nr 4
411-419
Spores of many fungal pathogens are dispersed by wind. Detection of these airborne inocula is important in forecasting both the onset and the risk of epiphytotics. Species-specific primers targeted at the internal transcribed spacer (ITS) region of Leptosphaeria maculans and L. biglobosa - the causal organisms of phoma stem canker and stem lesions of Brassica spp., including oilseed rape - were used to detect DNA extracted from particles deposited on tapes obtained from a spore trap operated in Rarwino (northwest Poland) from September to November in 2004 and 2006. The quantities of DNA assessed by traditional end-point PCR and quantitative real-time PCR were compared to microscopic counts of airborne ascospores. Results of this study showed that fluctuations in timing of ascospore release corresponded to the dynamics of combined concentrations of DNA from L. maculans and L. biglobosa, with significant positive correlations between ascospore number and DNA yield. Thus the utilization of PCR-based molecular diagnostic techniques enabled the detection, identification, and accurate quantification of airborne inoculum at the species level. Moreover, real-time PCR was more sensitive than traditional PCR, especially in years with low ascospore numbers.
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