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In 2008–2010, in the field experiment, the yield and quality of lavender flowers in organic cultivation were tested. The experiment was established in four different locations in Poland. The following features were evaluated: fresh and dried flower yields, seed yield, weight of 1 000 kernels, essential oil content and its composition and microbiological contamination. Lavender flower yield from organic experiments was higher compared with the yield from conventional cultivation. The content of essential oil in organic lavender flowers was lower then that from conventional ones. The content of essential oil and its composition of lavender flowers did not depend on localization. The satisfied yield of lavender seeds was obtained in organic experiments. The investigated lavender raw materials were below the level of standard contamination for raw materials treated with hot water (A) according to European Pharmacopoeia 7.0.
The quantity of the crop plant pests have to be under control. A defense against pest organisms with toxic chemicals has been the prevailing pest control strategy for over 50 years. However, plants were found to possess insecticidal properties. Several essential oils act as antifeedants, deterrents and repellents. Essential oils are products of the secondary metabolism in plants. They are mixtures of simple and volatile compounds. One of the example is lavender oil, which repels Ceutorhynchus sp. - the most important pests of Brassica napus var. oleifera plants. The same effect was observed in the case when linalool, linalyl acetate (main components of lavender oil) and lavandulol were applied [Duda, Dubert 2008a, 2008b]. Thymol, the major constituent of thyme oil, is also a deterrent for the lepidopteran pest - Plutella xylostella L. [Akhtar, Isman 2004].
The aim of the experiment conducted in 2003–2006 was to estimate the effect of dose of nitrogen on yielding and antioxidant activity of lavender. The experiment was established in one factorial design in four replications and plot area 3.0 m2. Nitrogen was supplied in three different rates: as a preplant dose 50 kg N·ha⁻¹, or split application 100 (50 + 50) and 200 kg N·ha⁻¹ (100 + 100) with one top dressing provided on first week of May. Results obtained from two years of the study indicate that the most suitable for lavender yielding appeared to be nitrogen fertilization at the rate of 100 kg N·ha⁻¹. The results of the study also demonstrated that heavy nitrogen fertilization decreased concentration of phenolic compounds and have negligible effect on chlorophyll and carotenoids concentration in lavender flowers and increased content of phenolics, total carotenoids and chlorophyll a + b in leaves. The antioxidant activity of lavender flowers was higher in treatment fertilized with nitrogen in dose of 50 kg N·ha⁻¹ and decreased at the rates of 100 and 200 kg N·ha⁻¹.
The aim of the study was to examine the antibiofilm activity of selected essential oils (EO): Lavandula angustifolia (LEO), Melaleuca alternifolia (TTO), Melissa officinalis (MEO) and some of their major constituents: linalool, linalyl acetate, α-terpineol, terpinen-4-ol. Biofilms were formed by Staphylococcus aureus ATCC 29213 and Escherichia coli NCTC 8196 on the surface of medical biomaterials (urinary catheter, infusion tube and surgical mesh). TTC reduction assay was used for the evaluation of mature biofilm eradication from these surfaces. Moreover, time-dependent eradication of biofilms preformed in polystyrene 96-well culture microplates was examined and expressed as minimal biofilm eradication concentration (evaluated by MTT reduction assay). TTO, α-terpineol and terpinen-4-ol as well as MEO, showed stronger anti-biofilm activity than LEO and linalool or linalyl acetate. Among the biomaterials tested, surgical mesh was the surface most prone to persistent colonization since biofilms formed on it, both by S. aureus and E. coli, were difficult to destroy. The killing rate studies of S. aureus biofilm treated with TTO, LEO, MEO and some of their constituents revealed that partial (50%) destruction of 24-h-old biofilms (MBEC₅₀) was achieved by the concentration 4-8×MIC after 1 h, whereas 2-4×MIC was enough to obtain 90% reduction in biomass metabolic activity (MBEC₉₀) after just 4 h of treatment. A similar dose-dependent effect was observed for E. coli biofilm which, however, was more susceptible to the action of phytochemicals than the biofilms of S. aureus. It is noteworthy that an evident decrease in biofilm cells metabolic activity does not always lead to their total destruction and eradication.
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