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The paper presents studies on level hydrolytic activity of extracellular enzymes in the surface microlayer and subsurface water in the coastal lake Dołgie Wielkie. The ranking order of the potential enzyme activity rates in the studied water layers was as follows: aminopeptidase > lipase > α-glucosidase > β-glucosidase. The level of activity of all studied hydrolases was higher in the surface microlayer than subsurface water. Activity of extracellular enzymes was influenced by the season.
Utilization of various amino acids and carbohydrates by neustonic and planktonic bacteria isolated from coastal Lake Dołgie Wielkie was determined. The highest percentage of the bacteria inhabiting this lake used proline and glutamine. Considerably higher percentage of neustonic bacteria (FL, SL) than planktonic (SUB) bacteria in their metabolic processes prefered the tested amino acids. The highest percentage of isolated bacteria utilized fructose and glucose. The majority of tested carbohydrates actively used neustonic bacteria isolated from surface layer (SL) than film layer (FL) and subsurface layer (SUB). There were no major differences in the intensity of assimilation of amino acids and carbohydrates between bacteria inhabiting particular parts of Lake Dołgie Wielkie.
Antibiotic resistance of bacteria was observed in various aquatic environments including seas, rivers, lakes, coastal areas, surface water and sediments. The increased implementation of antibiotics into these environments through medical therapy, agriculture and animal husbandry has resulted in new selective pressures on natural bacterial aquatic populations. Antibiotic resistance of heterotrophic bacteria isolated from the surface microlayer and subsurface water of freshwater coastal polymictic and low-productive lake was studied. Antibiotic resistance was determined by the single disc diffusion method. The resistance level of bacteria to various antibiotics differed considerably. Bacteria were most resistant to ampicillin, ciprofloxacin, clindamycin, erythromycin and penicillin. Majority of bacterial strains showed resistance to 4–6 out of 18 antibiotics tested. As a rule, neustonic bacteria (antibiotic resistance index, ARI 0.44) were more resistant to the studied antibiotics than planktonic bacteria (ARI 0.32). 70–90% of neustonic bacteria were resistant to ampicillin, clindamycin and erythromycin, 60– 70% of planktonic bacteria were resistant to ampicillin, ciprofloxacin and penicillin. Differences between pigmented and non-pigmented bacteria in their resistance to the tested antibiotics were observed. Above 40% of achromogenic bacterial strains were resistant to ampicillin, clindamycin and penicillin. Among bacterial strains characterised by their ability to synthesize carotenoids, more than 30% was resistant to ampicillin and ciprofloxacin. Bacterial resistance level to antibiotics depended on their chemical structure. Bacteria isolated from study lake were most resistant to quinolones and lincosamides while they were most susceptible to tetracyclines and aminoglycosides. Results presented in this paper indicate that antibiotics are a significant selection factor and probably play an important role in regulating the composition of bacterial communities in aquatic ecosystems. Adaptive responses of bacterial communities to several antibiotics observed in the present study may have possible implications for the public health.
For the period of 4 years, in quarter-time cycle, samples had been taken from surface microlayers and subsurface water of the lakes: Gardno, Jasień and Dołgie Wielkie, by two techniques (Garret net and glass plate). In each talked over layers we have analyzed contents of calcium, general alka-linity, conductance and water reaction (pH). We have also determined the ability of surface mi-crolayer to accumulate these components and we have compared it to subsurface water ability and verified whether this ability depends on the kind of the lake.
The aim of the work was to characterize the species composition of the fish assemblages in the littoral zone and to analyze catch statistics from the lobelia-lake Lake Dołgie Wielkie. Catches were conducted with a fry trawl between May and August 2001 in the shallow littoral. Commercial catches from 1952 to 1970 and 1980 to 1999 were also analyzed. Nine fish species were caught and the majority (81.1%) of specimens belonged to the juvenile stages. The greatest contribution (%) to the littoral fish assemblage was made by belica, which was also described by the highest availability coefficient. A small number of species which comprised the lake fish assemblage and the distribution of their frequency of occurrence meant that the Simpson coefficient (D) of species diversity was low at 0.2082. The littoral of Lake Dołgie Wielkie is not very diverse and the ichthyofauna diversity in it is dominated by belica. In comparison with lobelia-lakes in western Pomerania, the structure of the share of various species in commercial catches was different in Lake Dołgie Wielkie, and the fisheries yield was higher. The fish assemblage which inhabits the lake is characteristic of eutrophic lakes.
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