Phenolic acids from leaves and roots ofJovibarba sobolifera (Sims.) Opiz were analysed by means of the two-dimensional thin layer chromatography and by the RP-HPLC method. Twelve phenolic acids were identified by 2D-TLC: chlorogenic, gallic, protocatechuic, caffeic, p-coumaric, p-hydroxy- benzoic, p-hydroxyphenylacetic, vanillic, syringic, ferulic, synapic and a-rezorcylic. The RP-HPLC enabled describing the contents often major phenolic acids in the examined material.