Allene oxide synthase (AOS) encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA) formation. In this study we report the molecular cloning and characterization of InAOS from Ipomoea nil. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 motif, which is evolutionarily conserved and characteristic for functional enzymatic proteins. We have shown that wounding led to a strong stimulation of the examined gene activity in cotyledons and an increase in JA level, which suggest that this compound may be a modulator of stress responses in I. nil.
First and last instar larva and pupa of Laccoptera foveolata (Boheman, 1856) are described in detail along with notes on the life history. The beetles were observed to breed from June to August. The host plant of the insect is Ipomoea nil (Convolvulaceae), as the entire life cycle is completed on this plant. A small, oval ootheca containing a single egg is partially covered by fecal matter. There are five larval instars; the larvae and pupa are typical for the specialized cassidines. Imagines develop in about 35-40 days from the fertilized egg deposited in the ootheca.
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