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The aim of this study was to determine the effect of different initial doses of the infective juveniles (IJs) (50 IJs, 200 IJs, 1000 IJs) of Heterorhabditis megidis Poinar (Rhabditida: Heterorhabditidae) strain IsM15/09 on recovery, final yields and percent final yields in larvae Galleria mellonella ( L.). Percent recovery was not directly related to initial dose. Final yields also did not change with the initial dose. However, percent yields was highly negatively correlated with initial dose of nematodes and was the highest with the 50 IJs dose. Additional point of the study was to investigate whether the nematodes are able to produce progeny from one hermaphroditic individual. The results showed that the invasive larvae resumed growth and transformed into hermaphroditic individuals that reproduced without cross-fertilisation.
Sensitivity of imagines and larvae of the lesser mealworm to selected species and strains of entomopathogenic nematodes was studied in a pine sawdust litter on which chicken were kept from one to six weeks according to their production cycle (Niemiec 1998). The following nematode species and strains were used: S. feltiae from bioinsecticides Ovinema and Nemaplus, S. affinis, S. carpocapsae and H. bacteriophora strain Brecon. S. feltiae from biopreparation Ovinema appeared most invasive to the larvae and imagines of A. diaperinus.
Activity of infective juveniles of Heterorhabditis bacteriophora in pre- and post-parasitic phases was studied vis-a-vis malathion treatment. The vitality of dauers (J₃) was ascertained subsequent to exposure to various sublethal concentrations of malathion (incorporated into nematode rearing medium). At concentrations of 200 and 400 ppm, the dauers showed behavioural disruptions as indicated by incomplete undulations. Anterior half of the treated dauers were mainly involved in performing undulations as compared with the posterior half. Lower concentrations showed transient effects. Infectivity tests were conducted using filter paper bioassay. The parasitization rate in Spodoptera litura larvae (L₆) decreased with increasing concentrations of malathion. Post-parasitic juveniles were examined daily for assessing the viability of H. bacteriophora after the treatment of malathion. Because of the cogent infectivity after malathion-exposure, it is suggested, that this nematode might be used as an agent in IPM. It appears a promising candidate.
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