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The impact of artificial mycorrhization with 6 vegetative inocula (produced by research procedures at the Forest Pathology Department at Agricultural University in Cracow) of ectomycorrhizal fungi: Hebeloma claviceps, H. leucosarx, H. longicaudum, H. sacchariolens, H. sinapizans and H. trunkatum on following parameters: the level of mycorrhization of Scots pine and pedunculate oak seedlings, the profile of frequency and types of ectomycorrhizae on these seedlings, the influence of intervention on the growth and development characteristics of shoot and root system was studied. The results of the mycorrhization described above were compared with the mycorrhization with Polish commercial vegetative inoculum of Hebeloma crustuliniforme carried out on the seedlings of the same tree species grown in containers. The assessment revealed that all fungi species representing Hebeloma genus were very good symbionts for pine. The index of mycorrhization of the root system in each case was high and achieved level between 0,86 for H. longicaudum to 1,0 for H. sacchariolens and H. claviceps. The growth and development of oak seedlings in container culture were improved in the highest degree by H. crustuliniforme, H. leucosarx, H. sinapizans and H. sacchariolens.
In this experiment a few physiological characters were measured in order to show differences in leaf water potential (Ψ), chlorophyll content (SPAD) and chlorophyll a fluorescence (F0, FM, FV, FV/FM) between leaves differing in age of 3 poplar cultivars inoculated with H. crustuliniforme. In control plants (non-inoculated) differences in water potential and chlorophyll fluorescence parameters between the examined cultivars were slight. Statistically significant differences in water potential and chlorophyll content between non-inoculated and inoculated plants were observed only for young leaves from the upper parts of the plant stem. Similarly, significant differences between treatments in initial fluorescence (F0), maximal fluorescence (FM) and in variable fluorescence (FV) were found in leaves from the middle and upper part of the plant stem. The obtained results indicated that for leaves from the upper, median and lower part of the plant stem significant differences between treatments in maximum quantum yield of PS2 (FV/FM) in leaves from different plant parts in P. petrowskayana were not observed and in P. deltoides cv. Plantierens and P. balsamifera only for leaves from the upper parts of the plant stem. Although those differences between inoculated and non-inoculated plants were relatively small, we may presume, that if they occur through a longer period they may become more distinct causing positive changes in leaves. In conclusion, it is necessary to саrrу out future studies on the mechanism by which the EMC symbiosis influences leaf physiological traits.
The aim of the study was to assess the effect of the application of Sincocin AL and inoculation with the fungi Hebeloma crustuliniforme or Laccaria bicolor on the growth and level of mycorrhizal colonization of Scots pine seedlings. Sincocin had no effect on the development of biometric parameters of pine seedlings inoculated with L. bicolor, it limited the growth of non−inoculated seedlings and stimulated the growth of the seedlings from the variant with H. crustuliniforme. Sincocin did not cause changes in the proportion of the mycorrhizae formed by H. crustuliniforme or L. bicolor, and affected in different ways (stimulation, inhibition, no effect) the spontaneous formation of mycorrhizae.
The study evaluated the effect of inoculation with Hebeloma crustuliniforme and application of Biochikol 020 PC to the soil in three doses (single, double and triple) on the growth of Scots pine in the nursery and plantation. Both treatments stimulated the growth of seedlings. The effect of Biochikol was found only in the nursery irrespective of the used doses of the preparation. The effect of mycorrhization on pine growth was stronger compared to the application of Biochikol both in the nursery and plantation.
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