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Characterization of two lipopolysaccharide types isolated from Rhizobium galegae

100%

Rasanen L. A., Russa R., Urbaniak T., Choma A., Mayer H., Lindstrom K.

Acta Biochimica Polonica

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1997

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tom 44

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nr 4

Lipopolysaccharides (LPS) of Rhizobium galegae, a symbiotically nitrogen-fixing species of root-nodule bacteria, were isolated by the phenol-water method from strain HAMBI 1461, the LPS of which resembled enterobacterial smooth type LPS, and from strains HAMBI 1174 and HAMBI 1208, the LPSs of which resembled rough type LPS. The results of PAGE analysis of LPSs, Bio-Gel P2 gel filtration of polysaccharide fractions and the presence of deoxysugars and 4-O-methyl-deoxysugar both in the rough and smooth LPSs suggested that rough LPS contained a short O-antigenic polysaccharide for which we propose the name short O-chain LPS. Accordingly, the smooth LPS is called long O-chain LPS. Despite of the differences in the structure of LPS of R. galegae, all strains were equally effective in nodulating their hosts. The short O-chain LPS of R. galegae showed many features similar to those of phylogenetically related agrobacteria.

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Bacterial ghosts as multifunctional vaccine particles

88%

Lubitz W.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

3

Pest risk analysis on Xylella fastidiosa in Morocco

75%

Afechtal M., Vicent A., Saponari M., D’Onghia A. M.

Journal of Plant Protection Research

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2018

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tom 58

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nr 3

Morocco is basically an agricultural country; almost 40% of the workforce is employed in this sector. Xylella fastidiosa is a xylem-inhabiting pathogen which can infect more than 300 plant species, although most host species are symptomless. Until relatively recently, X. fastidiosa was primarily limited to North and South America, but in 2013 a widespread epidemic of olive quick decline syndrome caused by this fastidious pathogen appeared in southeastern Italy, and later several cases of X. fastidiosa outbreaks have been reported in other European countries (France, Germany and Spain). Following these recently confirmed findings of X. fastidiosa in the European Union, this bacterium has become a serious threat to the Moroccan flora. The national phytosanitary authorities have adopted several measures to prevent the introduction of X. fastidiosa into the national territory by deciding, inter alia, to suspend importation of host plant species to the bacterium from infected areas. This paper presents the phytosanitary risk of this bacterium in Morocco.

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Endotoxin levels as a potential marker of concentration of Gram-negative bacteria in water effluent from dental units and dental aerosols

75%

Szymanska J.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

229-232

Gram-negative bacteria concentration in water effluent from a dental unit, and in dental aerosol forming during the work of a dental handpiece, was assessed. The study was conducted on 25 dental units before and after a 2-week period of using a disinfecntant for water in dental units waterlines (DUWL). The contamination of water with Gram-negative bacteria before disinfection was 18-398 x 103 cfu/ml, and after disinfection, bacteria were not found. The concentration of Gram-negative bacteria in the air before disinfection was 0-23 x 101 cfu/m3, and after disinfection - 0-8 x 101 cfu/m3. Simultaneously, the water and air were sampled to determine bacterial endotoxin. The statistical analysis did not show correlation between endotoxin concentration and Gram-negative bacteria concentration for the water before disinfection, and for the air before and after disinfection of DUWL water. Because the number of bacteria in the water after disinfection dropped to zero, statistical methods could not be used. The performed analysis suggests that bacterial endotoxin concentration is not indicative of Gram-negative bacteria contamination. Thus, bacterial endotoxin determination is not recommended as a method of monitoring the microbiological quality of DUWL water and dental aerosols.

5

Biosynthesis of bacterial peptidoglycan

75%

Kloszewska M.

Acta Microbiologica Polonica

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1996

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tom 45

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nr 2

6

Liposome-encapsulated carbapenems

75%

Drulis-Kawa Z., Gubernator J., Kostyn K., Doroszkiewicz W., Kozubek A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2

7

Immunotherapy in Gram-negative bacterial infections

75%

Lugowski C.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

Endotoxins are responsible for initiation of septic shock which increases the number of fatalities in Gram-negative bacteremia among hospital patients. The morality from septic shock is still high despite recent developments in antibiotic therapy because antibiotics are unable to decrease the level of free lipopolysaccharide in the blood stream. Another approach to the treatment and prevention of septicaemia involves stimulation of an immune response against LPS. It was found that immunization with the core structures of endotoxin conjugated with proteins protected animals against infections and endotoxic shock. Anticonjugate sera are of great interest because they are directed against conserved parts of LPS and therefore could have cross-reactive and cross-protective properties with respect to many Gram-negative rods.

8

Proteolytic activity of Pseudomonas chlororaphis 5N isolated from rhizosphere soil

63%

Jankiewicz U., Frak M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2012

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tom 15

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nr 4

9

Co-existence of Legionella and other Gram-negative bacteria in potable water from various rural and urban sources

63%

Stojek N. M., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2011

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tom 18

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nr 2

10

The immune response in periodontal tissues

63%

Nedzi-Gora M., Kowalski J., Gorska R.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 5

11

Effect of external addition of rhamnolipids biosurfactant on the modification of gram positive and gram negative bacteria cell surfaces during biodegradation of hydrocarbon fuel contamination

63%

Kaczorek E.

Polish Journal of Environmental Studies

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2012

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tom 21

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nr 4

The bacterial strains from the genera: Bacillus, Pseudomonas, Aeromonas, Achromobacter, and Flavimonas isolated from soil contaminated with crude oil were the subject of studies. The effect of the addition of rhamnolipids on cell surface properties and the removal efficiency of diesel oil were investigated. Rhamnolipids caused the modification of cell surface properties of tested strains, which depended on the amount of external additions of biosurfactant. Additionally, the decrease of Zeta potential was observed after the introduction of rhamnolipids to the diesel oil system. Particle size distribution provides information about system homogeneity and the tendency of particles toward agglomeration. Cell surface hydrophobicity during hydrocarbon biodegradation is a dynamic parameter. There were no different effects, after the addition of rhamnolipids, on the Gram positive and Gram negative bacterial strains. Moreover, the addition of rhamnolipids does not always increase the efficiency of diesel oil biodegradation. The most effective bacterial strain in diesel oil removal was the genus Pseudomonas.

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Aerobic microflora of Myotis myotis [Borkhausen, 1797] and Barbastella barbastellus [Schreber, 1774]

63%

Rozalska B., Radzicki G., Sadowska B., Markowski J., Rudnicka W.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1998

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tom 46

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nr 2

13

Towards an occupational exposure limit for endotoxins

63%

Heederik D., Douwes J.

Annals of Agricultural and Environmental Medicine

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1997

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tom 04

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nr 1

14

Are NKT cells essential for endotoxic shock?

63%

Emoto M.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 4

15

Magainin 2 interaction with Pantoea agglomerans lipopolysaccharide

63%

De Lucca A. J., Jacks T. J., Brogden K. A.

Annals of Agricultural and Environmental Medicine

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1996

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tom 03

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nr 1

16

Bactericidal activity of normal human serum against Morganella, Proteus, and Providencia strains

63%

Nawrot U., Mokracka-Latajka G., Grzybek-Hryncewicz J., Krzyzanowska B., Jankowski S.

Acta Microbiologica Polonica

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1995

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tom 44

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nr 1

17

Effects of repeated inhalation exposure to microvesicle-bound endotoxin

63%

Skorska C., Sitkowska J., Burrell R., Szuster-Ciesielska A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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1996

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tom 03

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nr 1

18

Immunomodulation of macrophages by pathogenic Yersinia species

63%

Ruckdeschel K.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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tom 50

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nr 2

19

Allergic alveolitis among agricultural workers in eastern Poland: a study of twenty cases

63%

Milanowski J., Dutkiewicz J., Potoczna H., Kus L., Urbanowicz B.

Annals of Agricultural and Environmental Medicine

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1998

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tom 05

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nr 1

20

Purification of IHF-like protein from gram-negative bacteria in one chromatographic step

63%

Krawczyk B., Kur J.

Acta Biochimica Polonica

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1996

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tom 43

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nr 2

We describe a fast and very efficient method of purification which yields highly purified integration host factor-like proteins in one chromatografie step. IHF-like proteins from Acinetobacter junii or Proteus vulgaris are each an a£ heterodimer (subunits of 10 and 11 kDa) similar to the IHF of Escherichia coli when analyzed by polyacrylamide gel electrophoresis. The purified IHF are able to bind to the same ihf sites as IHF of E. coli. The results presented confirm that IHF is conserved during evolution in gram-negative bacteria.

Ograniczanie wyników

Characterization of two lipopolysaccharide types isolated from Rhizobium galegae