Wyniki wyszukiwania

1

Subcellular localization of UDP-GlcNAc, UDP-Gal and SLC35B4 transporters

100%

Maszczak-Seneczko D., Olczak T., Olczak M.

Acta Biochimica Polonica

|

2011

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tom 58

|

nr 3

The mechanisms of transport and distribution of nucleotide sugars in the cell remain unclear. In an attempt to further characterize nucleotide sugar transporters (NSTs), we determined the subcellular localization of overexpressed epitope-tagged canine UDP-GlcNAc transporter, human UDP-Gal transporter splice variants (UGT1 and UGT2), and human SLC35B4 transporter splice variants (longer and shorter version) by indirect immunofluorescence using an experimental model of MDCK wild-type and MDCK-RCAr mutant cells. Our studies confirmed that the UDP-GlcNAc transporter was localized to the Golgi apparatus only and its localization was independent of the presence of endogenous UDP-Gal transporter. After overexpression of UGT1, the protein colocalized with the Golgi marker only. When UGT2 was overexpressed, the protein colocalized with the endoplasmic reticulum (ER) marker only. When UGT1 and UGT2 were overexpressed in parallel, UGT1 colocalized with the ER and Golgi markers and UGT2 with the ER marker only. This suggests that localization of the UDP-Gal transporter may depend on the presence of the partner splice variant. Our data suggest that proteins involved in nucleotide sugar transport may form heterodimeric complexes in the membrane, exhibiting different localization which depends on interacting protein partners. In contrast to previously published data, both splice variants of the SLC35B4 transporter were localized to the ER, independently of the presence of endogenous UDP-Gal transporter.

2

Submicroscopical structure of parabasal body in Trichomonas vaginalis Donne

100%

Szreter H., Kassner J.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 4

3

The phospholipid contents in rat liver Golgi-rich membrane fractions after streptozotocin and/or prostaglandin treatment

100%

Kordowiak A. M.

Folia Histochemica et Cytobiologica

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1986

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tom 24

|

nr 1

4

Influence of D,L-ethionine intoxication on galactosyl transferase activity and morphology of the rat liver Golgi apparatus

100%

Kordowiak A., Kaczmarski F.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 1

5

Retention of glycosyltransferases in the Golgi apparatus

100%

Jaskiewicz E.

Acta Biochimica Polonica

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1997

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tom 44

|

nr 2

A number of Golgi glycosyltransferases has been cloned to date. They all are membrane proteins and share the same type II topology, but they do not possess an obvious sequence homology which would suggest a common Golgi retention signal. However, it was shown that the membrane-spanning domain and its flanking regions contain necessary and sufficient information for Golgi retention of these enzymes. Currently, two mutually complementary models have been proposed to explain the mechanism of Golgi retention of glycosyltransferases mediated by their transmembrane domain. The first model postulates the retention through oligomerization, which prevents enzymes from entering the transport vesicles. The second suggests that retention depends on the length of a membrane-spanning domain and thickness of the membrane along the Golgi complex. It has to be pointed out that neither the oligomerization nor the membrane thickness model alone can answer all questions and further work is still needed to elucidate the retention process of Golgi proteins.

6

Effect of long-term administration of melatonin on ultrastructure of pinealocytes in gilts

100%

Przybylska B., Lewczuk B., Dusza L., Wyrzykowski Z.

Folia Morphologica

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1994

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tom 53

|

nr 3

7

Myosin VI is associated with secretory granules and is present in the nucleus in adrenal medulla chromaffin cells

84%

Majewski L., Sobczak M., Redowicz M. J.

Acta Biochimica Polonica

|

2010

|

tom 57

|

nr 1

109-114

Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments. Here, MVI, but not myosins IB or IIB, was detected in chromaffin granules isolated from bovine medulla and found to be tightly associated with the granule apical surface. MVI also localized to secretory granules within rat pheochromocytoma PC12 cells as well as to the Golgi apparatus, endoplasmic reticulum and clathrin-coated pits. Notably, it was also found in the nucleus. RT-PCR revealed that MVI splice variants with a large insert (LI), characteristic of polarized cells, were barely detectable in PC12 cells, whereas variants with a small insert (SI) were the major isoforms. The presented data indicate that MVI in adrenal medulla cells is engaged in secretory vesicle trafficking within the cytoplasm and possibly also involved in transport within the nucleus.

8

Electron microscopical examination of the effects of Ra irradiation on the cytoskeleton elements of neoplastic cells of uterine cervix

84%

Sporniak M., Kertynska I., Wawrzkiewicz M., Karmowski A.

Folia Histochemica et Cytobiologica

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1986

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tom 24

|

nr 4

9

Studies on the influence of microtubular inhibitors on the secretory process in secretory cell of the mammary gland

84%

Sporniak M., Perissel B.

Folia Histochemica et Cytobiologica

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1986

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tom 24

|

nr 4

10

Locomotory response of Amoeba proteus to Hplus and a protonophore (FCCP)

84%

Dolowy K., Grebecki A.

Acta Protozoologica

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1988

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tom 27

|

nr 2

11

2-Deoxyglucose induces beta-APP overexpression, tau hyperphosphorylation and expansion of the trans-part of the Golgi complex in rat cerebral cortex

84%

Grieb P., Gordon-Krajcer W., Frontczak-Baniewicz M., Walski M., Ryba M. S., Kryczka T., Fiedorowicz M., Kulinowski P., Sulek Z., Majcher K., Jasinski A.

Acta Neurobiologiae Experimentalis

|

2004

|

tom 64

|

nr 4

12

Glycosylation defects corrected by the changes in GDPmannose level

84%

Kruszewska J., Janik A., Lenart U., Palamarczyk G.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 2

GDPMan is a key substrate in glycoprotein formation. This is especially true for lower eukaryotes where, in addition to the involvement in N-glycan biosynthesis and GPI-anchor formation, GDPMan takes part in the process which is unique for yeast and fungi i.e. O-mannosylation. Several lines of evidence have been presented that the level of GDPMan affects the process occurring in the Golgi compartment i.e. the elongation of outer mannose chain of glycoproteins in Saccharomyces cerevisiae. Results from our laboratory indicate that the availability of GDPMan affects also the early steps of glycoprotein formation ascribed to the endoplasmic reticulum, i.e. assembly of the dolichol-linked oligosaccharide as well as mannosyl-phosphodolichol (MPD) formation. The biochemical basis of carbohydrate deficient glycoprotein syndrome, a severe neurological disorder related to the GDPMan deficiency, is also discussed.

13

Expansion of the Golgi apparatus in rat cerebral cortex following intracerebroventricular injections of streptozotocin

84%

Grieb P., Kryczka T., Fiedorowicz M., Frontczak-Baniewicz M., Walski M.

Acta Neurobiologiae Experimentalis

|

2004

|

tom 64

|

nr 4

14

Prostaglandin E [dmPGE2] action in vitro on the activity of rat liver Golgi apparatus galactosyltransferase

67%

Kordowiak A. M., Tomecki J., Procyk K., Kapusta P.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 4

In vitro addition of 16,16'-dimethyl prostaglandin E2 to Golgi-rich membrane fraction in final concentration of 0.1 ng/1 mg of protein increased generally the activity of galactosyltransferase in comparison with control. The percentage of phospholipids in the whole fraction was similar in both investigated groups, only the sum of phosphatidylethanolamine + phosphatide acid was significantly lower after addition of dmPGE2 than in the control (0.001

Ograniczanie wyników

Subcellular localization of UDP-GlcNAc, UDP-Gal and SLC35B4 transporters

Submicroscopical structure of parabasal body in Trichomonas vaginalis Donne

The phospholipid contents in rat liver Golgi-rich membrane fractions after streptozotocin and/or prostaglandin treatment

Influence of D,L-ethionine intoxication on galactosyl transferase activity and morphology of the rat liver Golgi apparatus

Retention of glycosyltransferases in the Golgi apparatus

Effect of long-term administration of melatonin on ultrastructure of pinealocytes in gilts

Myosin VI is associated with secretory granules and is present in the nucleus in adrenal medulla chromaffin cells

Electron microscopical examination of the effects of Ra irradiation on the cytoskeleton elements of neoplastic cells of uterine cervix

Studies on the influence of microtubular inhibitors on the secretory process in secretory cell of the mammary gland

Locomotory response of Amoeba proteus to Hplus and a protonophore (FCCP)

2-Deoxyglucose induces beta-APP overexpression, tau hyperphosphorylation and expansion of the trans-part of the Golgi complex in rat cerebral cortex

Glycosylation defects corrected by the changes in GDPmannose level

Expansion of the Golgi apparatus in rat cerebral cortex following intracerebroventricular injections of streptozotocin

Prostaglandin E [dmPGE2] action in vitro on the activity of rat liver Golgi apparatus galactosyltransferase