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Effect of trypsin modulating oostatic factor (TMOF) on trypsin and chymotrypsin in Glyphodes pyloalis Walker (Lep.: Pyralidae) and Hyphantria cunea Drury (Lep.: Arctiidae)

100%

Ajamhassani M., Jalali Sendi J., Ghadamyary M., Borovsky D.

Pestycydy

2011

nr 1-4

In the present study, the effect of three concentrations of TMOF (7.5, 15 and 30 μg dissolved in water) on trypsin and chymotrypsin biosynthesis in 4th instar larvae of Glyphodes pyloalis walker and Hyphantria cunea Drury were studied 24 h and 48 h after injecting. Our results indicated that in G. pyloalis, lower concentrations (7.5 and 15 μg) inhibited trypsin biosynthesis at 24 h after injection. (P < 0.0001). TMOF, however, did not significantly affect trypsin biosynthesis at 48 h. In H. cunea, at 48 h after the injection, all concentrations (7.5, 15 and 30 μg per larvae) significantly inhibited trypsin biosynthesis (P < 0.05). Injections of TMOF did not significantly affect chymotrypsin in both insects. Although, in G. pyloalis, chymotrypsin activity decreased about 25% at 48 h after injection.

Effect of Artemisia annua L. on deterrence and nutritional efficiency of lesser mulberry pyralid (Glyphodes pylolais Walker) (Lepidoptera: Pyralidae)

100%

Khosravi R., Sendi J. J., Ghadamyari M.

Journal of Plant Protection Research

2010

tom 50

nr 4

Methanolic extract of Artemisia annua L. were evaluated on growth and nutritional efficiency of lesser mulberry pyralid Glyphodes pyloalis (Lepidoptera: Pyralidae) in laboratory conditions (24±1°C, 75±5% RH and 16 L : 8 D). Feeding deterrence index was evaluated by a choice test method on < 24 h fifth instar larvae with 5, 2.5, 1.25 and 0.625 percent of the extract. The result indicated that increasing the concentration resulted in higher deterrence. The extract retarded larval growth significantly (EC50 = 3.63%) and also showed significant effects on the relative growth rate (RGR), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), approximate digestibility (AD) and consumption index (CI). Lipid, protein and carbohydrate in treated insects were significantly reduced compared to the controls. Hence, methanol extract of A. annua could be considered as a strong deterrent which also affects the biochemical metabolism of the target pest. Methanol extract of A. annua has the potential for development as a botanical insecticide.

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Pestycydy

1 Ajamhassani M.

1 Borovsky D.

1 Ghadamyari M.

1 Ghadamyary M.

1 Jalali Sendi J.

1 Khosravi R.

1 Sendi J. J.

1 2011

1 2010

Wyniki wyszukiwania

Effect of trypsin modulating oostatic factor (TMOF) on trypsin and chymotrypsin in Glyphodes pyloalis Walker (Lep.: Pyralidae) and Hyphantria cunea Drury (Lep.: Arctiidae)

Effect of Artemisia annua L. on deterrence and nutritional efficiency of lesser mulberry pyralid (Glyphodes pylolais Walker) (Lepidoptera: Pyralidae)