Wyniki wyszukiwania

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New experimental and computational approaches to the analysis of gene expression

100%

Rafalski J. A., Hanafey M., Miao G. H., Ching A., Lee J. M., Dolan M., Tingey S.

Acta Biochimica Polonica

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1998

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tom 45

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nr 4

Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained. To estimate expression levels of genes represented in the DuPont EST data base we count the number of times each gene has been seen in different cDNA libraries, from different tissues, developmental stages or induction conditions. This quantitation of message levels is quite accurate for highly expressed messages and, unlike conventional Northern blots, allows comparison of expression levels between different genes. Lists of most highly expresses genes in different libraries can be compiled. Also, if EST data is available for cDNA libraries derived from different developmental stages, gene expression profiles across development can be assembled. We present an example of such a profile for soybean seed development. Gene expression data obtained from Electronic Northern analysis can be confirmed and extended beyond the realm of highly expressed genes by using high density DNA arrays. The ESTs identified as interesting can be arrayed on nylon or glass and probed with total labeled cDNA first strand from the tissue of interest. Two-color fluorescent labeling allows accurate mRNA ratio measurements. We are currently using the DNA array technology to study chemical induction of gene expression and the biosynthesis of oil, carbohydrate and protein in developing seeds. In the future, high density DNA arrays containing the whole gene complement of an organism will become available for the analysis of genome-wide patterns of gene expression.

2

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Identification of miRNAs and their potential targets in halophyte plant Thellungiella halophila

86%

Panahi B., Mohammadi S. A., Ebrahimie E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

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Expressed Sequence Tags and their application for plant research

86%

Kisiel A., Podkowinski J.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 2

Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST coltections comprise over 3.8 miliion sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based tools for data mining. Searching EST coltections allows pre-selection of genes for preparing cDNA arrays, targeted to bring maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, EST-based molecular markers such as SNP, SSR, and indels are fast developing tools for breeders and re searchers.

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Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution

72%

Idziak D., Hasterok R., Betekhtin A., Borowska-Zuchowska N., Braszewska-Zalewska A., Chwialkowska K., Gorkiewicz R., Kus A., Kwasniewska J., Kwasniewski M., Robaszkiewicz E., Siwinska D., Wolny E., Chrominski K., Tkacz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

Ograniczanie wyników

2 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Acta Biochimica Polonica

1 Acta Physiologiae Plantarum

1 Betekhtin A.

1 Borowska-Zuchowska N.

1 Braszewska-Zalewska A.

1 Ching A.

1 Chrominski K.

1 Chwialkowska K.

1 Dolan M.

1 Ebrahimie E.

1 Gorkiewicz R.

1 Hanafey M.

1 Hasterok R.

1 Idziak D.

1 Kisiel A.

1 Kus A.

1 Kwasniewska J.

1 Kwasniewski M.

1 Lee J. M.

1 Miao G. H.

1 Mohammadi S. A.

1 Panahi B.

1 Podkowinski J.

1 Rafalski J. A.

1 Robaszkiewicz E.

1 Siwinska D.

1 Tingey S.

1 Tkacz M.

1 Wolny E.

1 2015

1 2013

1 2005

1 1998

New experimental and computational approaches to the analysis of gene expression

Identification of miRNAs and their potential targets in halophyte plant Thellungiella halophila

Expressed Sequence Tags and their application for plant research

Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution