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Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.)

100%

Ramachandran K., Manaf U. A., Zakaria L.

Journal of Plant Protection Research

2015

tom 55

nr 4

The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.).The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR) fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC) and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

Fast molecular detection of Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, and Dickeya spp. from potato (Solanum tuberosum l.) stem tissues

100%

Oksinska M. P., Magnucka E. G., Pietr S. J.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

2016

tom 19

nr 4

Ograniczanie wyników

1 Electronic Journal of Polish Agricultural Universities. Series Agronomy

1 Journal of Plant Protection Research

1 Magnucka E. G.

1 Manaf U. A.

1 Oksinska M. P.

1 Pietr S. J.

1 Ramachandran K.

1 Zakaria L.

1 2016

1 2015

Wyniki wyszukiwania

Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.)

Fast molecular detection of Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, and Dickeya spp. from potato (Solanum tuberosum l.) stem tissues