Wyniki wyszukiwania

1

Protein kinase inhibition of CDK2 by staurosporine

100%

Lawrie A. M., Noble M. E. M., Tunnah P., Brown N. R., Johnson L. N., Endicott J. A.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

2

Autonomous replication of wheat DNA sequence in isolated wheat nuclei

88%

Szurmak B., Buchowicz J.

Acta Biochimica Polonica

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1997

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tom 44

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nr 1

A fragment of wheat nuclear DNA was shown to be able to replicate autonomously in wheat nuclei. The fragment was 637-bp long and contained telomeric repeats at both termini. Its replication was manifested by the appearance of a radioactive reaction product of the same approximate size. Further analyses showed that the linear, double-stranded réaction product was labelled along its entire length and contained the same number of similarly located HaelR sites as did the fragment tested. Prokaryotic DNA remained twlabelled under the same assay conditions.

3

Detection of novel DNA-nuclear matrix interactions

88%

Ferraro A., Altieri F., Cervoni L., Eufemi M., Coppari S., Turano C.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

4

DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

88%

Takahashi H., Yamaguchi T., Koga M., Kageura H., Terada S.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 3

Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.

5

A preliminary analysis of the region with limited variability in C-repeats of rabbit DNA

75%

Plaza G., Szemraj J., Bednarek A., Anderson P., Szala S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 4-6

6

The main DNA viruses significantly affecting pig livestock

75%

Diaz C., Celer V., Frebort I.

Journal of Veterinary Research

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2021

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tom 65

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nr 1

7

Functional aspects of nuclear matrix

75%

Wanka F.

Acta Biochimica Polonica

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1995

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tom 42

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nr 2

A model is proposed of the way in which the unwinding of the chromosomal DNA loops is controlled during DNA replication. It is based on the observation of a permanent binding of replication origins to the nuclear matrix and of a transient attachment of replicating DNA regions to sites in the immediate neighbourhood. DNA unwinding is controlled while the replicating loops are reeled through the replication binding sites. Also a mechanism is proposed to explain how the once-per-cycle replication of individual replicons can be controlled. DNA synthesis is initiated at single-stranded loops exposed by tandemly repeated DNA sequences at the replication origins. The single-stranded loops turn into fully double-stranded DNA during replication, becoming inaccessible for a second initiation during the same cell cycle. The configuration competent for initiation is restored by specific protein-DNA rearrangements coupled to mitotic condensation of the matrix into chromosomal scaffolds and its reversal.

8

Cell cycle checkpoints - molecular background

75%

Grzelakowska-Sztabert B.

Folia Morphologica

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2004

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tom 63

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nr 1

Cell cycle checkpoints are the surveillance mechanisms monitoring both the fidelity and accuracy of DNA replication and the segregation of chromosomes. By delaying progression through the cell cycle, checkpoints provide more time for repair before the critical phases of DNA replication and ensure the proper segregation of chromosomes during mitosis. The paper provides basic information about the molecular mechanisms operating in various cell cycle checkpoints activated by DNA damage or disturbances in mitotic spindle assembly.

9

Control of the cell cycle progression

75%

Grabarek J.

Biotechnologia

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1998

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nr 3

10

Non-random distribution of GATC sequences in regions of promoters stimulated by the SeqA protein of Escherichia coli

75%

Strzelczyk B., Slominska-Wojewodzka M., Wegrzyn G., Wegrzyn A.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 4

The SeqA protein of Escherichia coli is not only the main negative regulator of DNA replication initiation but also a specific transcription factor. It binds to hemi- methylated GATC sequences and, with somewhat different specificity, to fully methylated GATC regions. Recently, a microarray analysis was reported, in which transcriptomes of wild-type and AseqA strains were compared. Although in the ΔseqA mutant the levels of some transcripts were significantly decreased while certain transcripts were evidently more abundant relative to wild-type bacteria, no correlation between the presence of GATC motifs in promoter sequences and transcription activity was found. However, here we show that when larger DNA fragments, encompassing positions from -250 to +250 relative to the transcription start site, are analyzed, some common features of GATC distribution near the promoters activated by SeqA can be demonstrated. Nevertheless, it seems that the GATC pattern is not the only determinant of SeqA-dependence of promoter activity.

11

Probing nuclear envelope, pore, lamina assembly and checkpoint mechanisms in metazoans

75%

Smythe C., Jenkins H., Drummond S., Hall-Jackson C., Marshall B., Feijoo C., Hutchison C. J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

12

UV- and MMS-induced mutagenesis of lambdaO[am]8 phage under nonpermissive conditions for phage DNA replication

75%

Krwawicz J., Czajkowska A., Felczak M., Pietrzykowska I.

Acta Biochimica Polonica

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2003

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tom 50

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nr 4

Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop replication at the fork. Reinitiation needs translesion synthesis (TLS) by DNA polymerase V (UmuC), which in the presence of accessory proteins, UmuD', RecA and ssDNA-binding protein (SSB), has an ability to bypass the lesion with high mutagenicity. This enables reinitiation and extension of DNA replication by DNA polymerase III (Pol III). We studied UV- and MMS-induced mutagenesis of λO(am)8 phage in E. coli 594 sup host, unable to replicate the phage DNA, as a possible model for mutagenesis induced in nondividing cells (e.g. somatic cells). We show that in E. coli 594 sup cells UV- and MMS-induced mutagenesis of λO(am)8 phage may occur. This mutagenic process requires both the UmuD' and C proteins, albeit a high level of UmuD' and low level of UmuC seem to be necessary and sufficient. We compared UV-induced mutagenesis of λO(am)8 in nonpermissive (594 sup ) and permissive (C600 supE) conditions for phage DNA replication. It appeared that while the mutagenesis of XO(am)8 in 594 sup+ requires the UmuD' and C proteins, which can not be replaced by other SOS-inducible protein(s), in C600 supE their functions may be replaced by other inducible protein(s), possibly DNA polymerase IV (DinB). Mutations induced under nonpermissive conditions for phage DNA replication are resistant to mismatch repair (MMR), while among those induced under permissive conditions, only about 40% are resistant.

13

Combined effect of strigent or relaxed response, temperature and rom function on the replication of pUC plasmids in Escherichia coli

75%

Herman A., Wegrzyn A., Wegrzyn G.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

14

Involvement of the essential yeast DNA polymerases in induced gene conversion

75%

Halas A., Ciesielski A., Zuk J.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

In the yeast Saccharomyces cerevisiae three different DNA polymerases α, δ and ε are involved in DNA replication. DNA polymerase α is responsible for initiation of DNA synthesis and polymerases δ and ε are required for elongation of DNA strand during replication. DNA polymerases δ and ε are also involved in DNA repair. In this work we studied the role of these three DNA polymerases in the process of recombinational synthesis. Using thermo-sensitive heteroallelic mutants in genes encoding DNA polymerases we studied their role in the process of induced gene conversion. Mutant strains were treated with mutagens, incubated under permissive or restrictive conditions and the numbers of convertants obtained were compared. A very high difference in the number of convertants between restrictive and permissive conditions was observed for polymerases α and δ, which suggests that these two polymerases play an important role in DNA synthesis during mitotic gene conversion. Marginal dependence of gene conversion on the activity of polymerase ε indicates that this DNA polymerase may be involved in this process but rather as an auxiliary enzyme.

15

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Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen

75%

Szyfter K., Wiktorowicz K., Wielgosz M. S., Zajaczek S., Kujawski M., Jaloszynski P., Czub M., Markowska J.

Journal of Applied Genetics

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1995

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tom 36

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nr 4

The effects of mutagens on DNA replication and DNA repair were studied in peripheral blood lymphocytes (PBL) obtained from 21 healthy subjects, 2 samples from healthy heterozygote of Xeroderma pigmentosum (XP) and 2 samples from patient with clinically recognised XP. Inter-individual variations were found in DNA replication and in the level of spontaneous DNA repair measured under standard culture condition. Exposure of human PBL proliferating in vitro to B(a)P was followed by a partial inhibition of replicative DNA synthesis in all subjects and by an induction of DNA repair in healthy subjects. In XP patients DNA repair synthesis remained at the level attributed to spontaneous DNA repair. The response to mutagen varied individually. Results were analysed statistically. It was established that the studied indices of DNA synthesis correlate well with each other. The highest correlation was found between the levels of spontaneous and B(a)P-induced DNA repair. It is concluded that the level of spontaneous DNA repair is predictive for an estimation of cells ability to repair DNA damage. Inter-individual variations in the inhibition of DNA replication and in DNA repair synthesis are also dependent on the type of mutagen as shown by effects of other mutagens. Different effects of mutagen exposure on the inhibition of DNA replicative synthesis and induction of DNA repair can be explained by genetically controlled differences in the activity of enzymes responsible for mutagen processing and lesion removal.

16

Prenatal detection of maternal UPD15 in new case with i[15p] by Timing Replication Test [TRT] and methylation analysis

75%

Constantinou M., Kaluzewski B., Helszer Z., Zajac E., Nowacka J.

Journal of Applied Genetics

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2003

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tom 44

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nr 2

17

Expression of c-fos, c-myc and hsp70 genes at early stages of the regenerating of rat liver

63%

Biesiada E., Wisniewski J., Krawczyk Z., Chorazy M. M.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1987

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tom 35

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nr 7-9

18

Study of DNA topoisomerase IIalpha expression in canine lymphomas and its potential role as a marker of sensitivity to anthracycline-based chemotherapy in dogs

63%

Klimiuk P., Lopuszynski W., Bulak K., Smiech A., Brzana A.

Folia Histochemica et Cytobiologica

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2020

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tom 58

|

nr 1

19

Identification of cell cycle proteins interacting with Arabidopsis thaliana proliferating cell nuclear antigen using bimolecular fluorescence complementation assay

63%

Strzalka W., Jakubowska A., Bartnicki F., Pels K., Kowalska E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

20

DNA replication stress-induced biphasic nuclear structures in Allium cepa root meristem cells

63%

Zabka, Polit J. T., Maszewski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

Ograniczanie wyników

Protein kinase inhibition of CDK2 by staurosporine

Autonomous replication of wheat DNA sequence in isolated wheat nuclei

Detection of novel DNA-nuclear matrix interactions

DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

A preliminary analysis of the region with limited variability in C-repeats of rabbit DNA

The main DNA viruses significantly affecting pig livestock

Functional aspects of nuclear matrix

Cell cycle checkpoints - molecular background